The Role of Nitrogen and Phosphorous Source in Miscanthus in vitro Cultures
The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crops with broad applications, e.g. for fuel and energy, for thatching, fibre production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962) basic medium supplemented with 0,3 mg l−1 6-Benzylaminopurin). After 40 days of culturing the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that after 35 days, nitrogen and phosphorous was nearly completely taken up. From that time, shoot growth was not observed.
After the shoot propagation the plants were transferred into a nutrient solution for root formation basic medium (modified Murashige and Skoog, 1962) supplemented with 0,5 mg l−1 IBA ( Indol-3-Butyric Acid ), and could be potted into soil, after about 14 days.
Key wordsMiscanthus nitrogen phosphorous in vitro culture
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