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Cereal Research Communications

, Volume 42, Issue 1, pp 47–57 | Cite as

TaWRKY71, a WRKY Transcription Factor from Wheat, Enhances Tolerance to Abiotic Stress in Transgenic Arabidopsis thaliana

  • Q. Xu
  • W. J. Feng
  • H. R. Peng
  • Z. F. NiEmail author
  • Q. X. SunEmail author
Genetics

Abstract

Members of WRKY gene family encode transcription factors involved in plant developmental processes and response to biotic and abiotic stresses. In order to understand the function of the TaWRKY71 gene, a homologue gene was isolated and characterised in wheat (Triticum aestivum L.) genotype TAM107. Tissue-specific gene expression profiles indicated that TaWRKY71 was constitutively expressed in roots, stems, leaves, stamen and pistil. The relative expression of TaWRKY71 was elucidated under ABA treatment and other abiotic stresses. In agreement with this, several putative cis-acting elements involved in ABA-response, drought-inducibility, low-temperature and heat response were detected in the promoter region of TaWRKY71. The function of TaWRKY71 was further determined by transforming Arabidopsis thaliana. Transgenic plants over-expressing TaWRKY71 displayed enhanced seed germination under ABA treatment and were tolerant to salt and drought stresses. These results indicate that TaWRKY71 gene might play important roles in seed germination and abiotic stress response.

Keywords

wheat TaWRKY71 seed germination abiotic stress 

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijingChina
  2. 2.Cotton Research InstituteShanXi Academy of Agricultural ScienceYunChengChina

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