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Cereal Research Communications

, Volume 42, Issue 1, pp 102–110 | Cite as

Characterization of a Phytase (TaPAPhy_a1.1) Gene in an Indian Wheat Cultivar

  • A. Verma
  • S. Ram
  • S. Dalal
Physiology

Abstract

A full length phytase (TaPAPhy_a1.1) gene was cloned and sequenced from Indian wheat cultivar DBW17. The 2,060 bp sequence has four introns of 63, 90, 94, 103 bp and encodes a polypeptide of 548 amino acids. N-terminal residues 5 to 19 contain a signaling peptide (SP) targeted to the vacuole. The predicted molecular weight and isoelectric point were 60.53 kDa and 6.74, respectively. A phylogenetic tree analysis revealed that TaPAPhy_a1.1 clustered on a branch with PAPhy_a1 from Aegilops tauschii cultivar NGB 9855. A structural model of TaPAPhy_a1.1 protein showed a single chain dimer containing two metal ions (Fe and Zn) bound at its active site. It belongs to the metallophosphoesterase group containing a characteristic set of seven amino acid residues (Asp, Asp, Tyr, Asn, His, His, His) involved in metal ligation. The full length TaPAPhy_a1.1 gene sequence from this investigation can be used for allele mining studies to identify superior alleles for higher phytase levels for improving the nutritional quality of wheat.

Keywords

nutritional quality purple acid phosphatase Triticum aestivum 

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Copyright information

© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Verma
    • 1
  • S. Ram
    • 1
  • S. Dalal
    • 2
  1. 1.Directorate of Wheat ResearchKarnalIndia
  2. 2.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia

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