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Cereal Research Communications

, Volume 41, Issue 3, pp 458–467 | Cite as

Distribution of 1AL.1RS and 1BL.1RS Wheat-rye Translocations in Iranian Wheat, Using PCR Based Markers and SDS-PAGE

  • N. Tabibzadeh
  • G. KarimzadehEmail author
  • M. R. Naghavi
Quality and Utilization
  • 1 Downloads

Abstract

Short arm of rye chromosome 1 (1RS) in wheat (Triticum aestivum L.) improvement is being widely utilized by many plant breeders. The 1BL.1RS translocation derived from the Russian wheat cultivar “Kavkaz”, carring genes for major wheat diseases such as stem, strip and leaf rusts and powdery mildew resistance. The 1AL.1RS translocation derived from “Amigo”, possessing resistance genes for stem rust, powdery mildew and greenbug. The distribution of the wheat-rye translocations 1BL.1RS and 1AL.1RS was studied in 44 Iranian wheat cultivars (29 bread wheat cultivars and 15 durum wheats). In this study the presence of the translocations was identified in 5 cultivars (Dez, Atrak, Rasul, Falat and Moghan3), using SDS-PAGE technique and 3 DNAmarkers based PCR. The both results of PCR based markers and SDS-PAGE showed that the frequency of the 1BL.1RS in Iranian bread wheat is very low (5 cultivars of bread wheat) and 1AL.1RS did not exist in Iranian wheat backgrounds. Such techniques are quick and reliable tools to recognize and to distinguish these two wheat-rye translocations in wheat genetic background.

Keywords

wheat-rye translocation 1BL.1RS 1AL.1RS Triticum aestivu Secale cereale Iran SDS-PAGE DNA marker 

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Plant Breeding and Biotechnology Department, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Agronomy and Plant Breeding Department, Agricultural CollegeUniversity of TehranKarajIran

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