Cereal Research Communications

, Volume 41, Issue 2, pp 243–254 | Cite as

Agro-Biochemical Traits of Wheat Genotypes under Irrigated and Non-Irrigated Conditions

  • N. KhanEmail author
  • F. N. NaqviEmail author


The objective of current study was to look at the variable expression of antioxidant enzymes in wheat genotypes exposed to various water stress regimes. Further the malondialdehyde (MDA) content were measured as an indicative of membrane integrity. Tolerance indices were calculated which reinforce in distinguishing tolerant and susceptible genotypes. The experimental material consisted of thirteen genotypes obtained from different sources. Stress was imposed by withholding irrigation at three different growth stages of plant, i.e. tillering, anthesis and grain filling. Four resistance indices include stress susceptibility index (SSI), yield stability index (YSI), mean productivity (MP) and tolerance index (TOL) was calculated on the basis of grain yield. Water stress treatments had no significant effect on CAT activity. CIM-47, CIM-49 and NR-234 showed minimum MDA content with increased POX activity under three different irrigation conditions and are therefore considered as tolerant genotypes. Higher levels of MDA with decline activity of POX was found in CIM-51, DD-4 and NR-230 led to suggest them as susceptible genotypes. The variable response of genotypes in tolerance could be related to differences in antioxidant enzyme levels. Significant positive correlation was found between SSI and TOL values whereas negative and significant association was noted between SSI and YSI. Significant and negative correlation was observed between YSI and TOL values. These traits are recognized as beneficial water stress tolerance indicators for selecting a stress tolerant variety. The most outstanding tolerance capacity in terms of susceptibility indices was detected in CIM-47 and CIM-50 under all water stresses. They indicated lowest SSI, TOL and MP with high YSI values. It may, therefore, be concluded that these genotypes have the potential of stress tolerance.


antioxidant enzymes MDA resistance indices water stress 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abedi, T., Pakniyat, H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech J. Genet. Plant Breed. 46:27–34.CrossRefGoogle Scholar
  2. Aebi, H. 1984. Catalase in vitro. Methods in Enzymology 105:121–126.CrossRefGoogle Scholar
  3. Anjum, S.A., Xie, X.Y., Wang, L.C., Saleem, M.S., Man, C., Lei, W. 2011. A review. Morphological, physiological and biochemical responses of plants to drought stress. Afr. J. Agric. Res. 6:2026–2032.Google Scholar
  4. Anwar, J., Subhani, G.M., Hussain, M., Ahmad, J., Hussain, M., Munir, M. 2011. Drought tolerance indices and their correlation with yield in exotic wheat genotypes. Pak. J. Bot. 43:1527–1530.Google Scholar
  5. Bouslama, M., Schapaugh, W.T. 1984. Stress tolerance in soybean. I. Evaluation of three screening techniques for heat and drought tolerance. Crop Sci. 24:933–937.CrossRefGoogle Scholar
  6. Bruckner, P.L., Frohberg, R.C. 1987. Stress tolerance and adaptation in spring wheat. Crop Sci. 27:31–36.CrossRefGoogle Scholar
  7. Dacosta, M., Huang, B. 2007. Changes in antioxidant enzyme activities and lipid peroxidation for bent grass species in responses to drought stress. J. Amer. Soc. Hort. Sci. 132:319–326.CrossRefGoogle Scholar
  8. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A. 1981. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased level of superoxide dismutase and catalase. J. Exp. Bot. 32:93–101.CrossRefGoogle Scholar
  9. Everse, J., Johnson, M.C., Marini, M.A. 1994. Peroxidative activities of hemoglobin and hemoglobin derivatives. In: Everse, J., Vandegriff, K.D., Winslow, R.M. (eds), Methods in Enzymology. Academic Press, London, UK, pp. 547–561.Google Scholar
  10. Fischer, R.A., Maurer, R. 1978. Drought resistance in spring wheat cultivars. I. Grain yield responses. Aust. J. Agric. Res. 29:897–912.CrossRefGoogle Scholar
  11. Hossain, A.B.S., Sears, A.G., Cox, T.S., Paulsen, G.M. 1990. Dessication tolerance and its relationship to assimilate partitioning in winter wheat. Crop Sci. 30:622–627.CrossRefGoogle Scholar
  12. Iqbal, S., Bano, A. 2009. Water stress induced changes in antioxidant enzymes, membrane stability and seed protein profile of different wheat accessions. Afr. J. Biotech. 8:6576–6587.Google Scholar
  13. Khan, N., Naqvi, F.N. 2010. Effect of water stress on lipid peroxidation and antioxidant enzymes in local bread wheat hexaploids. JFAE 82:521–526.Google Scholar
  14. Li, B., Wei, J., Wei, X., Tang, K., Liang, Y., Shu, K., Wang, B. 2008. Effect of sound wave stress on antioxidant enzyme activities and lipid peroxidation of Dendrobium candidum. Colloids Surf. B: Biointerfaces 63:269–275.CrossRefGoogle Scholar
  15. Mahajan, S., Tuteja, N. 2005. Cold, salinity and drought stresses: An overview. Arch. Biochem. Biophys. 444:139–158.CrossRefGoogle Scholar
  16. Miller, G., Suzuki, N., Ciftci-Yilmaz, S., Mittler, R. 2010. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ. 33:453–467.CrossRefGoogle Scholar
  17. Moaveni, P. 2011. Effect of water deficit stress on some physiological traits of wheat (Triticum aestivum). Agric. Sci. Res. J. 1:64–68.Google Scholar
  18. Moller, I.M., Jensen, P.E., Hansson, A. 2007. Oxidative modifications to cellular components in plants. Ann. Rev. Plant Biol. 58:459–481.CrossRefGoogle Scholar
  19. Monneveux, P., Rekika, D., Acevedo, E., Merah, O. 2006. Effect of drought on leaf gas exchange, carbon isotope discrimination, transpiration efficiency and productivity in field grown durum wheat genotypes. Plant Sci. 170:867–872.CrossRefGoogle Scholar
  20. Reddy, A.R., Chaitanya, K.V., Vivekanandan, M. 2004. Drought-induced response of photosynthesis and antioxidant metabolism in higher plants. J. Plant Physiol. 161:1189–1202.CrossRefGoogle Scholar
  21. Shao, H.B., Liang, Z.S., Shao, M.A. 2005. Changes of anti-oxidative enzymes and MDA content under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at maturation stage. Colloids Surf. B: Biointerfaces 45:7–13.CrossRefGoogle Scholar
  22. Sio-Se Mardeh, A., Ahmadi, A., Poustini, K., Mohammadi, V. 2006. Evaluation of drought resistance indices under various environmental conditions. Field Crop Res. 98:222–229.CrossRefGoogle Scholar
  23. Talebi, R., Fayaz, F., Naji, A.M. 2009. Effective selection criteria for assessing drought stress tolerance in durum wheat (Triticum Durum Desf.). Gen. Appl. Plant Physiol. 35:64–74.Google Scholar
  24. Xu, S., Li, J., Zhang, X., Wei, H., Cui, L. 2006. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultra structure of chloroplasts in two cool season turf grass species under heat stress. Environ. Exp. Bot. 56:274–285.CrossRefGoogle Scholar
  25. Yang, F., Miao, L.F. 2010. Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica 44:23–37.Google Scholar
  26. Zhang, J., Kirkham, M.B. 1994. Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol. 35:785–791.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of GeneticsUniversity of KarachiKarachiPakistan
  2. 2.Antrim CrescentTorontoCanada

Personalised recommendations