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Acta Biologica Hungarica

, Volume 65, Issue 4, pp 439–450 | Cite as

Effects of Temperature — Heavy Metal Interactions, Antioxidant Enzyme Activity and Gene Expression in Wheat (Triticum aestivum L.) Seedlings

  • N. ErgünEmail author
  • S. Özçubukçu
  • M. Kolukirik
  • Ö. Temizkan
Article

Abstract

In this study, the effect of heat and chromium (Cr) heavy metal interactions on wheat seedlings (Triticum aestivum L. cv. Ç-1252 and Gun91) was investigated by measuring total chlorophyll and carotenoid levels, catalase (CAT) and ascorbate peroxidase (APX) antioxidant enzyme activities, and MYB73, ERF1 and TaSRG gene expression. Examination of pigment levels demonstrated a decrease in total chlorophyll in both species of wheat under combined heat and heavy metal stress, while the carotenoid levels showed a slight increase. APX activity increased in both species in response to heavy metal stress, but the increase in APX activity in the Gun91 seedlings was higher than that in the Ç-1252 seedlings. CAT activity increased in Gun91 seedlings but decreased in Ç-1252 seedlings. These results showed that Gun91 seedling had higher resistance to Cr and Cr + heat stresses than the Ç-1252 seedling. The quantitative molecular analyses implied that the higher resistance was related to the overexpression of TaMYB73, TaERF1 and TaSRG transcription factors. The increase in the expression levels of these transcription factors was profound under combined Cr and heat stress. This study suggests that TaMYB73, TaERF1 and TaSRG transcription factors regulate Cr and heat stress responsive genes in wheat.

Keywords

Heat chromium wheat MYB73 ERF1 TaSRG antioxidant enzyme 

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • N. Ergün
    • 1
    Email author
  • S. Özçubukçu
    • 1
  • M. Kolukirik
    • 2
  • Ö. Temizkan
    • 1
  1. 1.Science and Art Faculty, Biology DepartmentMustafa Kemal UniversityAntakya, HatayTurkey
  2. 2.ENGY Environmental and Energy Technologies, Biotechnology Research and Development Limited Company34342 Bogazici UniversityBebek, İstanbulTurkey

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