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Acta Biologica Hungarica

, Volume 65, Issue 3, pp 265–273 | Cite as

Immunohistochemical Staining Reveals C-Reactive Protein Existing Predominantly as Altered Conformation Forms in Inflammatory Lesions

  • Ping Shi
  • Xiao-Xin Li
  • Wei Zhu
  • Hong Yang
  • Chi Dong
  • Xiao-Ming LiEmail author
Article

Abstract

C-reactive protein (CRP) is an established marker of inflammation and has been proposed to play a proinflammatory role in pathologies of several diseases. CRP is primarily produced by the liver and released into circulation as a pentameric molecule composed of five identical subunits. It has been suggested that the activation of the proinflammatory actions of CRP requires sequential conformational changes triggered by local inflammatory conditions. These include the dissociation into the subunit form (monomeric CRP, mCRP) and further reduction of the intra-subunit disulfide bond of mCRP. This model predicts that mCRP is the primary isoform present in inflamed but not healthy tissues, however the supporting evidence is lacking. Herein, we stained tissue samples across multiple anatomical locations from several types of human diseases with highly selective monoclonal antibodies that can differentiate CRP and mCRP. The results indicated that mCRP is the predominant form existing in the lesions. Further immunoblotting of the patient tissue samples revealed the potential presence of reduced mCRP. Together, we conclude that mCRP but not CRP is the major isoform present in local inflammatory lesions, supporting the so-called cascading model of CRP function and regulation.

Keywords

C-reactive protein conformational changes inflammation 

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ping Shi
    • 1
  • Xiao-Xin Li
    • 1
  • Wei Zhu
    • 1
  • Hong Yang
    • 1
  • Chi Dong
    • 1
  • Xiao-Ming Li
    • 1
    Email author
  1. 1.The Second Hospital of Lanzhou UniversityLanzhouPR China

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