The present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.
Benschop, J. J., Bou, J., Peeters, A. J. M., Wagemaker, N., Gühl, K., Ward, D., Hedden, P., Moritz, T., Voesenek, L. A. C. J. (2006) Long-term submergence-induced elongation in Rumex palustris requires abscisic acid-dependent biosynthesis of gibberellin1. Plant Physiol. 141, 1644–1652.
Biddington, N. L. (1992) The influence of ethylene in plant tissue cultures. Plant Growth Regul. 11, 173–187.
Bittsánszky, A., Gyulai, G., Gullner, G., Kiss, J., Szabó, Z., Kátay, G., Heszky, L., Kömíves, T. (2009) In vitro breeding of grey poplar (Populus × canescens) for phytoremediation purposes. J. Chem. Technol. Biotechnol. 84, 890–894.
Buddendorf-Joosten, J. M. C., Woltering, E. J. (1994) Components of the gaseous environment and their effects on plant growth and development in vitro. Plant Growth Regul. 15, 1–16.
Castiglione, S., Todeschini, V., Franchin, C., Torrigiani, P., Gastaldi, D., Cicatelli, A., Rinaudo, C., Berta, G., Biondi, S., Lingua, G. (2009) Clonal differences in survival capacity, copper and zinc accumulation, and correlation with leaf polyamine levels in poplar: a large-scale field trial on heavily polluted soil. Environ. Pollut. 157, 2108–2117.
Csabai, J., Nagy, Z., Mándy, A. T. (2011) In vitro shoot proliferation of Telekia speciosa (Schreb.) Baumg. induced by different cytokinins. Acta Biol. Hung. 62, 453–462.
Dimasi-Theriou, K., Economou, A. S., Sfakiotakis, E. M. (1993) Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene. Plant Cell Tiss. Organ Cult. 32, 219–225.
El Meskaoui, A., Tremblay, F. M. (1999) Effects of sealed and vented gaseous microenvironments on the maturation of somatic embryos of black spruce with a special emphasis on ethylene. Plant Cell Tiss. Organ Cult. 56, 201–209.
El Meskaoui, A., Tremblay, F. M. (2001) Involvement of ethylene in the maturation of black spruce embryogenic cell lines with different maturation capacities. J. Exp. Bot. 52, 761–769.
Eriksson, M. E., Israelsson, M., Olsson, O., Moritz, T. (2000) Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length. Nat. Biotechnol. 18, 784–788.
González, A., Arigita, L., Majada, J., Sánchez-Tamés, R. (1997) Ethylene involvement in in vitro organogenesis and plant growth of Populus tremula L. Plant Growth Regul. 22, 1–6.
Haver, D. L., Schuch, U. K., Lovatt, C. J. (2002) Exposure of petunia seedlings to ethylene decreased apical dominance by reducing the ratio of auxin to cytokinin. J. Plant Growth Regul. 21, 459–468.
Jackson, M. B. (2003) Aeration stress in plant tissue cultures. Bulg. J. Plant Physiol. 29, 96–109.
Kevers, C., Boyer, N., Courduroux, J. C., Gaspar, T. (1992) The influence of ethylene on proliferation and growth of rose shoot cultures. Plant Cell Tiss. Organ Cult. 28, 175–181.
Khattab, S. (2011) Effect of different media and growth regulators on the in vitro shoot proliferation of aspen, hybrid aspen and white poplar male tree and molecular analysis of variants in micropropagated plants. Life Sci. J. 8, 177–184.
Lai, C. C., Yeh, S. D., Yang, J. S. (2000) Enhancement of papaya axillary shoot proliferation in vitro by controlling the available ethylene. Bot. Bull. Acad. Sinica 41, 203–212.
Lambardi, M., Benelli, C., Fabbri, A. (1997) In vitro axillary shoot proliferation of apple rootstocks under different ethylene conditions. In Vitro Cell. Dev. Biol. Plant 33, 70–74.
Magdalita, P. M., Godwin, I. D., Drew, R. A., Adkins, S. W. (1997) Effect of ethylene and culture environment on development of papaya nodal cultures. Plant Cell Tiss. Organ Cult. 49, 93–100.
McCown, B. H., Lloyd, G. (1981) Woody plant medium (WPM)–a mineral nutrient formulation for microculture of woody plant species. HortScience 16, 453.
Naik, S. K., Chand, P. K. (2003) Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). J. Plant Physiol. 160, 423–430.
Panizza, M., Mensuali-Sodi, A., Tognoni, F. (1993) Role of ethylene in axillary shoot proliferation of lavandin–interaction with benzyladenine and polyamines. J. Exp. Bot. 44, 387–394.
Pereira-Netto, A. B. (2001) Effect of inhibitors of ethylene biosynthesis and signal transduction pathway on the multiplication of in vitro-grown Hancornia speciosa. Plant Cell Tiss. Organ Cult. 66, 1–7.
Peternel, Š., Gabrovšek, K., Gogala, N., Regvar, M. (2009) In vitro propagation of European aspen (Populus tremula L.) from axillary buds via organogenesis. Sci. Hortic. 121, 109–112.
Pintarić, B. (2008) Micropropagation of white poplar (Populus alba L.). Šumarski List 132, 343–354. (In Croatian)
Rademacher, W. (2000) Growth retardants: effects on gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51, 501–531.
Rédei, K. (2000) Early performance of promising white poplar (Populus alba) clones in sandy ridges between the rivers Danube and Tisza in Hungary. Forestry 73, 407–413.
Rédei, K., Keserű, Z., Gábor, S. (2010) Early evaluation of promising white poplar (Populus alba L.) clones in Hungary. Acta Silv. Lign. Hung. 6, 9–16.
Rijnders, J. G. H. M., Yang, Y. Y., Kamiya, Y., Takahashi, N., Barendse, G. W. M., Blom, C. W. P. M., Voesenek, L. A. C. J. (1997) Ethylene enhances gibberellin levels and petiole sensitivity in floodingtolerant Rumex palustris but not in flooding intolerant R. acetosa. Planta 203, 20–25.
Rosso, L., Facciotto, G., Bergante, S., Vietto, L., Nervo, G. (2013) Selection and testing of Populus alba and Salix spp. as bioenergy feedstock: preliminary results. Appl. Energy 102, 87–92.
Santos-del-Blanco, L., De-Lucas, A. I., González-Martínez, S. C., Sierra-de-Grado, R., Hidalgo, E. (2013) Extensive clonal assemblies in Populus alba and Populus × canescens from the Iberian Peninsula. Tree Genet. Genomes 9, 499–510.
Shimizu-Sato, S., Mori, H. (2001) Control of outgrowth and dormancy in axillary buds. Plant Physiol. 127, 1405–1413.
Tsvetkov, I., Jouve, L., Hoffmann, L., Hausman, J. F. (2007) The medium composition differentially affects regrowth characteristics in in vitro-derived encapsulated shoot tips of Populus euphratica Oliv. Propag. Ornam. Plants 7, 180–183.
Wan, X., Landhäusser, S. M., Lieffers, V. J., Zwiazek, J. J. (2006) Signals controlling root suckering and adventitious shoot formation in aspen (Populus tremuloides). Tree Physiol. 26, 681–687.
Welch, B. L. (1947) The generalization of ‘Student’s’ problem when several different population variances are involved. Biometrika 34, 28–35.
Zobayed, S. M. A., Armstrong, J., Armstrong, W. (2002) Multiple shoot induction and leaf and flower bud abscission of Annona cultures as affected by types of ventilation. Plant Cell Tiss. Organ Cult. 69, 155–165.
Žiauka, J., Kuusienė, S. (2010) Different inhibitors of the gibberellin biosynthesis pathway elicit varied responses during in vitro culture of aspen (Populus tremula L.). Plant Cell Tiss. Organ Cult. 102, 221–228.
Žiauka, J., Kuusienė, S., Šilininkas, M. (2013) Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action. Biomass Bioenergy 53, 20–28.
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Žiauka, J., Kuusienė, S. Multiplication and Growth of Hybrid Poplar (Populus Alba × P. Tremula) Shoots on a Hormone-Free Medium. BIOLOGIA FUTURA 65, 346–354 (2014). https://doi.org/10.1556/ABiol.65.2014.3.10
- Culture tube
- restricted ventilation
- white poplar