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Acta Biologica Hungarica

, Volume 64, Issue 3, pp 364–376 | Cite as

Phytohormone and Assimilate Profiles in Emasculated Flowers of the Black Locust (Robinia pseudoacacia) during Development

  • Peng Sun
  • Cunquan Yuan
  • Li Dai
  • Yang Xi
  • Yunfei Li
  • Ruiyang Hu
  • Yuhan Sun
  • Zhaohe Xu
  • Yun LiEmail author
Article
  • 3 Downloads

Abstract

Emasculation and bagging of flowers, which are widely used in the controlled pollination of monoclinous plants, may induce premature senescence, flower abscission and low fruit set. To determine the mechanism responsible for these phenomena, levels of abscisic acid (ABA), jasmonic acid (JA), indole-3-acetic acid (IAA), ethylene, soluble sugars, reducing sugars and free amino acids in black locust (Robinia pseudoacacia) flowers subjected to different treatments were quantified at different developmental stages. The phytohormones and assimilates were also quantified in untreated flowers to investigate the presence of discernible patterns. The levels of ethylene and ABA in emasculated and bagged (EB) flowers increased prematurely compared with those of untreated flowers, whereas the content of reducing sugars in EB flowers decreased compared with that of untreated flowers. These results indicated that the premature increase in ethylene and ABA synthesis, and the decrease in reducing sugars content, in EB flowers may cause flower abscission and result in low fruit set, which may be relevant for assimilate applications and future research on the regulation of controlled pollinations with exogenous phytohormones.

Keywords

Robinia pseudoacacia emasculation controlled pollination flower senescence phytohormones and assimilates 

Abbreviations

ABA

abscisic acid

EB

emasculation and bagging

GC-MS

gas chromatographymass spectrometry

IAA

indole-3-acetic acid

JA

jasmonic acid

RIE

response to injury induced by emasculation

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Notes

Acknowledgements

The authors thank the National Natural Science Foundation of China (31170629) for financial support, and Prof. Xiangning Jiang, Ms Huajun Chen and Miss Zhaolin Lv for technical assistance.

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Peng Sun
    • 1
  • Cunquan Yuan
    • 1
  • Li Dai
    • 1
  • Yang Xi
    • 1
  • Yunfei Li
    • 1
  • Ruiyang Hu
    • 1
  • Yuhan Sun
    • 1
  • Zhaohe Xu
    • 1
  • Yun Li
    • 1
    Email author
  1. 1.National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingChina

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