Variability of Stomata and 45S and 5S rDNAs Loci Characteristics in two Species of Anthoxanthum genus: A. Aristatum and A. Odoratum (Poaceae)


Diploid Anthoxanthum odoratum and tetraploid A. aristatum were compared with respect to stomatal guard cell lengths, and stomatal density at adaxial and abaxial surfaces of the lamina. Further, the genome size of both species was determined by flow cytometry, and the number as well as the chromosomal distribution of 5S and 45S rDNAs were examined using FISH with ribosomal DNA (rDNA) probes. The average length of stomatal guard cells in A. odoratum was shown to be greater than that for A. aristatum, but the ranges overlapped. Moreover, reduction in stomatal frequency was found at higher ploidy levels.The genome size was 6.863 pg/2C DNA for A. aristatum and 13.252 pg/2C DNA for A. odoratum. A. aristatum has four sites of 5S rDNA in its root-tip meristematic cells, whereas A. odoratum has six. Both species have six sites of 45S rDNA. Chromosomal localization of the rDNA varied, which suggests that chromosome rearrangements took place during Anthoxanthum genome evolution.


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The authors are grateful to Dr Robert Palmer Jr. (NIDCR, Bethesda), for his helpful comments on the manuscript. We also thank Mrs Dorota Siwinska (University of Silesia, Katowice) for her technical assistance with flow cytometry. This study has been supported by the Polish Ministry of Science and Higher Education (research project no. N N303069034).

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Correspondence to Maria Drapikowska.

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Drapikowska, M., Susek, K., Hasterok, R. et al. Variability of Stomata and 45S and 5S rDNAs Loci Characteristics in two Species of Anthoxanthum genus: A. Aristatum and A. Odoratum (Poaceae). BIOLOGIA FUTURA 64, 352–363 (2013).

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  • Ribosomal DNA
  • chromosome rearrangements
  • genome size
  • FISH