Acta Biologica Hungarica

, Volume 64, Issue 3, pp 377–384 | Cite as

Pre-Culturing of Nodal Explants in Thidiazuron Supplemented Liquid Medium Improves in vitro Shoot Multiplication of Cassia angustifolia

  • I. SiddiqueEmail author
  • N. Abdullwahab Bukhari
  • K. Perveen
  • I. Siddiqui
  • M. Anis


An in vitro propagation system for Cassia angustifolia Vahl. has been developed. Due to the presence of sennosides, the demand of this plant has increased manyfold in global market. Multiple shoots were induced by culturing nodal explants excised from mature plants on a liquid Murashige and Skoog [8] medium supplemented with 5–100 μM of thidiazuron (TDZ) for different treatment duration (4, 8, 12 and 16 d). The optimal level of TDZ supplemented to the culture medium was 75 μM for 12 d induction period followed by subculturing in MS medium devoid of TDZ as it produced maximum regeneration frequency (87%), mean number of shoots (9.6 ± 0.33) and shoot length (4.4 ± 0.46 cm) per explant. A culture period longer than 12 d with TDZ resulted in the formation of fasciated or distorted shoots. Ex vitro rooting was achieved when the basal cut end of regenerated shoots was dipped in 200 μM indole- 3-butyric acid (IBA) for half an hour followed by their transplantation in plastic pots filled with sterile soilrite where 85% plantlets grew well and all exhibited normal development. The present findings describe an efficient and rapid plant regeneration protocol that can further be used for genetic transformation studies.


Ex vitro Fabaceae growth characteristics tissue culture soilrite 


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The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project No. RGP-VPP-066.


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© Akadémiai Kiadó, Budapest 2013

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Authors and Affiliations

  • I. Siddique
    • 1
    Email author
  • N. Abdullwahab Bukhari
    • 1
  • K. Perveen
    • 1
  • I. Siddiqui
    • 1
  • M. Anis
    • 2
  1. 1.Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical CollegesKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Plant Biotechnology Laboratory, Department of Botany, Faculty of Life SciencesAligarh Muslim UniversityAligarhIndia

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