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Acta Biologica Hungarica

, Volume 64, Issue 2, pp 218–230 | Cite as

Efficient Plant Regeneration of Yellow Loosestrife (Lysimachia Vulgaris L.), a Medicinal Plant

  • Arzu Ucar Turker
  • Birgul Guner
Open Access
Article

Abstract

Lysimachia vulgaris L. (yellow loosestrife) is a medicinal plant that has been used in the treatment of fever, ulcer, diarrhea and wounds in traditional medicine. A reliable in vitro culture protocol for yellow loosestrife was established. Explants (leaf lamina, stem internode and root segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium supplemented with various plant growth regulator combinations. Of the tested combinations, those involving benzyladenine (BA) with either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) were the most effective for all used explants in shoot production. Best shoot proliferation was obtained from leaf lamina explant cultured on media containing 0.5 mg/l BA and 0.1, 0.5 or 1 mg/l IBA, from stem internode explant cultured on media containing 1 mg/ l BA and 0.5 mg/l IBA or 0.01 mg/l thidiazuron (TDZ) and 0.5 mg/l IAA, and from root explant cultured on media containing 0.5 mg/l BA and 0.5 mg/l IAA. Regenerated shoots were rooted on MSMO medium containing different concentrations of IAA, IBA, 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid (NAA). IBA was determined as the most effective auxin for rooting. Most shoots developed roots on medium with 0.5 mg/l IBA.

Keywords

Growth regulators in vitro culture Lysimachia vulgaris micropropagation yellow loosestrife 

References

  1. 1.
    Chaturvedi, H. C., Jain, M., Kidwai, N. R. (2007) Cloning of medicinal plants through tissue culture- A review. Indian J. Exp. Biol. 45, 937–948.PubMedGoogle Scholar
  2. 2.
    Chevallier, A. (1996) The Encyclopedia of Medicinal Plants. Darling Kindersley Limited, London.Google Scholar
  3. 3.
    Davis, P. H. (1978) Flora of Turkey and the East Aegean Islands, vol. 6. Edinburgh University Press, Edinburgh.Google Scholar
  4. 4.
    Dobelis, I. N. (1990) Magic and Medicine of Plants. The Reader’s Digest Association, New York.Google Scholar
  5. 5.
    Grieve, M. (1982) A Modern Herbal, vol. 2. Dover Publications, New York.Google Scholar
  6. 6.
    Hao, G., Yuan, Y. M., Hu, C. M., Ge, X. J., Zhao, N. X. (2004) Molecular phylogeny of Lysimachia (Myrsinaceae) based on chloroplast trnL-F and nuclear ribosomal ITS sequences. Mol. Phylogenet. Evol. 31, 323–339.CrossRefGoogle Scholar
  7. 7.
    Huetteman, C. A., Preece, J. E. (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult. 33, 105–119.CrossRefGoogle Scholar
  8. 8.
    Janik, I., Janeczko, Z., Sendra, J., Burczyk, J. (1994) Benzoquinone pigment from Lysimachia vulgaris. Fitoterapia 65, 476.Google Scholar
  9. 9.
    Kumar, N., Anand, K. G. V., Reddy, M. P. (2011) Plant regeneration of non-toxic Jatropha curcasimpacts of plant growth regulators, source and type of explants. J. Plant Biochem. Biot. 20, 125–133.CrossRefGoogle Scholar
  10. 10.
    Lu, C. Y. (1993) The use of TDZ in tissue culture. In vitro Cell. Dev. Biol. Plant 29, 92–96.CrossRefGoogle Scholar
  11. 11.
    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
  12. 12.
    Mutlu, E. C., Turker, A. U. (2008) Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant. Acta Soc. Bot. Pol. 77, 275–280.Google Scholar
  13. 13.
    Oh, I. C., Anderberg, A. L., Schonenberg, J., Anderberg, A. A. (2008) Comparative seed morphology and character evolution in the genus Lysimachia (Myrsinaceae) and related taxa. Plant Syst. Evol. 271, 177–197.CrossRefGoogle Scholar
  14. 14.
    Podolak, I., Strzalka, M. (2008) Qualitative and quantitative LC profile of embelin and rapanone in selected Lysimachia species. Chromatographia 67, 471–475.CrossRefGoogle Scholar
  15. 15.
    Podolak, I., Elas, M., Cieszka, K. (1998) In vitro antifungal and cytotoxic activity of triterpene saponosides and quinoid pigments from Lysimachia vulgaris L. Phytother. Res. 12, 70–73.CrossRefGoogle Scholar
  16. 16.
    Rout, G. R., Samantaray, S., Das, P. (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol. Adv. 18, 91–120.CrossRefGoogle Scholar
  17. 17.
    Rzadkowskabodalska, H., Olechnowiczstepien, W. (1975) Flavonoids in herb of yellow loosestrife (Lysimachia vulgaris L.). Pol. J. Pharmacol. Phar. 27, 345–348.Google Scholar
  18. 18.
    Siddique, I., Anis, M. (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia Vahl.: a medicinal plant. Acta Physiol. Plant. 29, 233–238.CrossRefGoogle Scholar
  19. 19.
    Trigiano, R. N., Gray, D. J. (2000) Plant Tissue Culture: Concepts and Laboratory Exercises. CRC Press, Boca Raton.Google Scholar
  20. 20.
    Turker, A. U., Yucesan, B., Gurel, E. (2009) An efficient in vitro regeneration system for Lythrum salicaria. Biol. Plantarum 53, 750–754.CrossRefGoogle Scholar
  21. 21.
    Wang, Y., Du, H. Y., Liang, H. Y., Liu, G. L. (2008). Tissue culture and plant regeneration from the leaves of Lysimachia christinae. J. Northwest Forestry Univ. 23, 104.Google Scholar
  22. 22.
    Yasukawa, K., Takido, M. (1988) Studies of the constituents of genus Lysimachia. 3.quercetin 3-rhamnosyl (1-]2) galactoside from Lysimachia vulgaris var. davurica. Phytochem. 27, 3017–3018.CrossRefGoogle Scholar
  23. 23.
    Yildirim, A. B., Turker, A. U. (2009) In vitro adventitious shoot regeneration of the medicinal plant meadowsweet (Filipendula ulmaria (L.) Maxim). In vitro Cell. Dev. Biol. Plant 45, 135–144.CrossRefGoogle Scholar
  24. 24.
    Yucesan, B., Turker, A. U., Gurel, E. (2007) TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell Tissue Organ Cult. 91, 243–250.CrossRefGoogle Scholar
  25. 25.
    Zheng, W., Xu, X., Dai, H., Chen, L. (2009) Direct regeneration of plants derived from in vitro cultured shoot tips and leaves of three Lysimachia species. Sci. Hortic. 122, 138–141.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Arzu Ucar Turker
    • 1
  • Birgul Guner
    • 1
  1. 1.Department of Biology, Faculty of Science and ArtAbant Izzet Baysal UniversityBoluTurkey

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