Efficient Plant Regeneration of Yellow Loosestrife (Lysimachia Vulgaris L.), a Medicinal Plant

Abstract

Lysimachia vulgaris L. (yellow loosestrife) is a medicinal plant that has been used in the treatment of fever, ulcer, diarrhea and wounds in traditional medicine. A reliable in vitro culture protocol for yellow loosestrife was established. Explants (leaf lamina, stem internode and root segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium supplemented with various plant growth regulator combinations. Of the tested combinations, those involving benzyladenine (BA) with either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) were the most effective for all used explants in shoot production. Best shoot proliferation was obtained from leaf lamina explant cultured on media containing 0.5 mg/l BA and 0.1, 0.5 or 1 mg/l IBA, from stem internode explant cultured on media containing 1 mg/ l BA and 0.5 mg/l IBA or 0.01 mg/l thidiazuron (TDZ) and 0.5 mg/l IAA, and from root explant cultured on media containing 0.5 mg/l BA and 0.5 mg/l IAA. Regenerated shoots were rooted on MSMO medium containing different concentrations of IAA, IBA, 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid (NAA). IBA was determined as the most effective auxin for rooting. Most shoots developed roots on medium with 0.5 mg/l IBA.

References

  1. 1.

    Chaturvedi, H. C., Jain, M., Kidwai, N. R. (2007) Cloning of medicinal plants through tissue culture- A review. Indian J. Exp. Biol. 45, 937–948.

    CAS  PubMed  Google Scholar 

  2. 2.

    Chevallier, A. (1996) The Encyclopedia of Medicinal Plants. Darling Kindersley Limited, London.

    Google Scholar 

  3. 3.

    Davis, P. H. (1978) Flora of Turkey and the East Aegean Islands, vol. 6. Edinburgh University Press, Edinburgh.

    Google Scholar 

  4. 4.

    Dobelis, I. N. (1990) Magic and Medicine of Plants. The Reader’s Digest Association, New York.

    Google Scholar 

  5. 5.

    Grieve, M. (1982) A Modern Herbal, vol. 2. Dover Publications, New York.

    Google Scholar 

  6. 6.

    Hao, G., Yuan, Y. M., Hu, C. M., Ge, X. J., Zhao, N. X. (2004) Molecular phylogeny of Lysimachia (Myrsinaceae) based on chloroplast trnL-F and nuclear ribosomal ITS sequences. Mol. Phylogenet. Evol. 31, 323–339.

    CAS  Article  Google Scholar 

  7. 7.

    Huetteman, C. A., Preece, J. E. (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult. 33, 105–119.

    CAS  Article  Google Scholar 

  8. 8.

    Janik, I., Janeczko, Z., Sendra, J., Burczyk, J. (1994) Benzoquinone pigment from Lysimachia vulgaris. Fitoterapia 65, 476.

    CAS  Google Scholar 

  9. 9.

    Kumar, N., Anand, K. G. V., Reddy, M. P. (2011) Plant regeneration of non-toxic Jatropha curcasimpacts of plant growth regulators, source and type of explants. J. Plant Biochem. Biot. 20, 125–133.

    Article  Google Scholar 

  10. 10.

    Lu, C. Y. (1993) The use of TDZ in tissue culture. In vitro Cell. Dev. Biol. Plant 29, 92–96.

    Article  Google Scholar 

  11. 11.

    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    CAS  Article  Google Scholar 

  12. 12.

    Mutlu, E. C., Turker, A. U. (2008) Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant. Acta Soc. Bot. Pol. 77, 275–280.

    CAS  Google Scholar 

  13. 13.

    Oh, I. C., Anderberg, A. L., Schonenberg, J., Anderberg, A. A. (2008) Comparative seed morphology and character evolution in the genus Lysimachia (Myrsinaceae) and related taxa. Plant Syst. Evol. 271, 177–197.

    Article  Google Scholar 

  14. 14.

    Podolak, I., Strzalka, M. (2008) Qualitative and quantitative LC profile of embelin and rapanone in selected Lysimachia species. Chromatographia 67, 471–475.

    CAS  Article  Google Scholar 

  15. 15.

    Podolak, I., Elas, M., Cieszka, K. (1998) In vitro antifungal and cytotoxic activity of triterpene saponosides and quinoid pigments from Lysimachia vulgaris L. Phytother. Res. 12, 70–73.

    Article  Google Scholar 

  16. 16.

    Rout, G. R., Samantaray, S., Das, P. (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol. Adv. 18, 91–120.

    CAS  Article  Google Scholar 

  17. 17.

    Rzadkowskabodalska, H., Olechnowiczstepien, W. (1975) Flavonoids in herb of yellow loosestrife (Lysimachia vulgaris L.). Pol. J. Pharmacol. Phar. 27, 345–348.

    CAS  Google Scholar 

  18. 18.

    Siddique, I., Anis, M. (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia Vahl.: a medicinal plant. Acta Physiol. Plant. 29, 233–238.

    CAS  Article  Google Scholar 

  19. 19.

    Trigiano, R. N., Gray, D. J. (2000) Plant Tissue Culture: Concepts and Laboratory Exercises. CRC Press, Boca Raton.

    Google Scholar 

  20. 20.

    Turker, A. U., Yucesan, B., Gurel, E. (2009) An efficient in vitro regeneration system for Lythrum salicaria. Biol. Plantarum 53, 750–754.

    CAS  Article  Google Scholar 

  21. 21.

    Wang, Y., Du, H. Y., Liang, H. Y., Liu, G. L. (2008). Tissue culture and plant regeneration from the leaves of Lysimachia christinae. J. Northwest Forestry Univ. 23, 104.

    Google Scholar 

  22. 22.

    Yasukawa, K., Takido, M. (1988) Studies of the constituents of genus Lysimachia. 3.quercetin 3-rhamnosyl (1-]2) galactoside from Lysimachia vulgaris var. davurica. Phytochem. 27, 3017–3018.

    CAS  Article  Google Scholar 

  23. 23.

    Yildirim, A. B., Turker, A. U. (2009) In vitro adventitious shoot regeneration of the medicinal plant meadowsweet (Filipendula ulmaria (L.) Maxim). In vitro Cell. Dev. Biol. Plant 45, 135–144.

    Article  Google Scholar 

  24. 24.

    Yucesan, B., Turker, A. U., Gurel, E. (2007) TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell Tissue Organ Cult. 91, 243–250.

    CAS  Article  Google Scholar 

  25. 25.

    Zheng, W., Xu, X., Dai, H., Chen, L. (2009) Direct regeneration of plants derived from in vitro cultured shoot tips and leaves of three Lysimachia species. Sci. Hortic. 122, 138–141.

    CAS  Article  Google Scholar 

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Turker, A.U., Guner, B. Efficient Plant Regeneration of Yellow Loosestrife (Lysimachia Vulgaris L.), a Medicinal Plant. BIOLOGIA FUTURA 64, 218–230 (2013). https://doi.org/10.1556/ABiol.64.2013.2.8

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Keywords

  • Growth regulators
  • in vitro culture
  • Lysimachia vulgaris
  • micropropagation
  • yellow loosestrife