Acta Biologica Hungarica

, Volume 64, Issue 1, pp 21–33 | Cite as

Chemical Composition and in vitro Antioxidant Activity of Hydro-Ethanolic Extracts from Bauhinia forficata subsp. Pruinosa and B. variegata

  • Carla T. M. Sayago
  • Vanessa B. de Camargo
  • F. Barbosa
  • Cláudia Gularte
  • Geovana Pereira
  • Silvia Miotto
  • V. Cechinel Filho
  • R. Luiz Puntel
  • V. Folmer
  • A. MendezEmail author
Open Access


Bauhinia species are known to have hypoglycemiant and antioxidant activities. Here, hydro-ethanolic leaf extracts from Bauhinia forficata subsp. pruinosa and Bauhinia variegata, collected in a Pampa biome region of Brazil, were investigated to characterize their chromatographic profile, flavonoid content and in vitro antioxidant activity (TBARS and DPPH assays). The extracts were obtained from dried and fresh leaves. The total flavonoid content was assessed by spectrophotometric determination, and the results ranged between 572.08 and 1,102.99 μg mL−1. Moreover, flavonoids were more predominant in B. variegata than in B. forficata subsp. pruinosa. HPLC analysis detected a complex profile of phenolic compounds, being the flavonoid kaempferitrin founded B. forficata subsp. pruinosa; in addition, other kaempferol and quercetin derivatives were present. In vitro antioxidant assays demonstrated a different behavior depending on the species, leaf treatment and extract concentration. In general, B. variegata extracts obtained from fresh material presented higher antioxidant potential, which can be attributed to the predominance of flavonoids in their chemical composition.


Bauhinia species flavonoids HPLC analysis antioxidant activity 


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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Carla T. M. Sayago
    • 1
  • Vanessa B. de Camargo
    • 1
  • F. Barbosa
    • 1
  • Cláudia Gularte
    • 2
  • Geovana Pereira
    • 2
  • Silvia Miotto
    • 3
  • V. Cechinel Filho
    • 4
  • R. Luiz Puntel
    • 2
    • 5
  • V. Folmer
    • 2
    • 5
  • A. Mendez
    • 1
    • 5
    Email author
  1. 1.Laboratório de Pesquisa em Desenvolvimento e Controle de Qualidade de MedicamentosUniversidade Federal do PampaUruguaianaBrazil
  2. 2.Laboratório de Bioquímica e Toxicologia de Produtos Naturais e SintéticosUniversidade Federal do PampaUruguaianaBrazil
  3. 3.Departamento de BotânicaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  4. 4.Núcleo de Investigações Químico-FarmacêuticasUniversidade do Vale do ItajaíItajaíBrazil
  5. 5.Programa de Pós-graduação em BioquímicaUniversidade Federal do PampaUruguaianaBrazil

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