Chemical Composition and in vitro Antioxidant Activity of Hydro-Ethanolic Extracts from Bauhinia forficata subsp. Pruinosa and B. variegata

Abstract

Bauhinia species are known to have hypoglycemiant and antioxidant activities. Here, hydro-ethanolic leaf extracts from Bauhinia forficata subsp. pruinosa and Bauhinia variegata, collected in a Pampa biome region of Brazil, were investigated to characterize their chromatographic profile, flavonoid content and in vitro antioxidant activity (TBARS and DPPH assays). The extracts were obtained from dried and fresh leaves. The total flavonoid content was assessed by spectrophotometric determination, and the results ranged between 572.08 and 1,102.99 μg mL−1. Moreover, flavonoids were more predominant in B. variegata than in B. forficata subsp. pruinosa. HPLC analysis detected a complex profile of phenolic compounds, being the flavonoid kaempferitrin founded B. forficata subsp. pruinosa; in addition, other kaempferol and quercetin derivatives were present. In vitro antioxidant assays demonstrated a different behavior depending on the species, leaf treatment and extract concentration. In general, B. variegata extracts obtained from fresh material presented higher antioxidant potential, which can be attributed to the predominance of flavonoids in their chemical composition.

References

  1. 1.

    Agrawal, R. C., Pandey, S. (2009) Evaluation of anticarcinogenic and antimutagenic potential of Bauhinia variegata extract in Swiss albino mice. Asian Pac. J. Cancer Prev. 10, 913–916.

    CAS  PubMed  Google Scholar 

  2. 2.

    Arygony, A. L. V. (2005) Determinação química e biológica de Bauhinia forficata Link subespécie pruinosa (Pata de Vaca - Leguminosae). Universidade Federal do Rio Grande do Sul, Brazil.

    Google Scholar 

  3. 3.

    Azevedo, C. R., Maciel, F. M., Silva, L. B., Ferreira, A. T. S., Da Cunha, M., Machado, O. L. T., Fernandes, K. V. S., Oliveira, A. E. A., Xavier-Filho, J. (2006) Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata. Braz. J. Med. Biol. Res. 39, 1435–1444.

    CAS  Article  Google Scholar 

  4. 4.

    Bortoluzzi, R. L. C., Miotto, S. T. S., Reis, A. (2006) Leguminosas-cesalpinioídeas. Herbário Barbosa Rodrigues, Itajaí.

    Google Scholar 

  5. 5.

    Cechinel Filho, V. (2009) Chemical composition and biological potential of plants from the genus Bauhinia. Phytother Res. 23, 1347–1354.

    CAS  Article  Google Scholar 

  6. 6.

    Choi, C. W., Kim, S. C., Hwang, S. S., Choi, B. K., Ahn, H. J., Lee, M. Y., Park, S. H., Kim, S. K. (2002) Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci. 153, 1161–1168.

    Article  Google Scholar 

  7. 7.

    Da Cunha, A. M., Menon, S., Menon, R., Couto, A. G., Bürger, C., Biavatti, M. W. (2010) Hypoglycemic activity of dried extracts of Bauhinia forficata Link. Phytomedicine 17, 37–41.

    Article  Google Scholar 

  8. 8.

    Da Silva, K. L., Biavatti, M. L., Leite, S. N., Yunes, R. A., Monache, F. D., Cechinel Filho, V. (2000) Phytochemical and pharmacognostic investigation of Bauhinia forficata Link (Leguminosae). Z Naturforsch. [C] 55, 478–480.

    Article  Google Scholar 

  9. 9.

    Da Silva, K. L., Cechinel Filho, V. (2002) Plantas do genero Bauhinia: composição química e poten-cial farmacológico. Quím. Nova 25, 449–454.

    Article  Google Scholar 

  10. 10.

    De Sousa, E., Zanatta, L., Seifriz, I., Creczynski-Pasa, T. B., Pizzolatti, M. G., Szpoganicz, B., Silva, F. R. M. B. (2004) Hypoglycemic effect and antioxidant potential of kaempferol-3,7-0-dirhamnoside from Bauhinia forficata leaves. J. Nat. Pro. 67, 829–832.

    Article  Google Scholar 

  11. 11.

    Farmacopéia Brasileira. (2010) Farmacopéia Brasileira-Métodos Gerais. ANVISA, Brasilia.

    Google Scholar 

  12. 12.

    Frioni, L., Dodera, R., Malatés, D., Irigoyen, I. (1998) An assessment of nitrogen fixation capability of leguminous trees in Uruguay. App. Soil. Ecol. 7, 271–279.

    Article  Google Scholar 

  13. 13.

    Fuentes, O., Arancibia-Avila, P., Alarcon, J. (2004) Hypoglycemic activity of Bauhinia candicans in diabetic induced rabbits. Fitoterapi. 75, 527–532.

    CAS  Article  Google Scholar 

  14. 14.

    Gupta, A. K., Vidyapati, T. J., Chauhan, J. S. (2010) Chemical examination of stem of Bauhinia variegata. Planta Med. 38, 174–176.

    Article  Google Scholar 

  15. 15.

    Jorge, A. P., Horst, H., Sousa, E., Pizzolatti, M. G., Barreto Silva, F. R. M. (2004) Insulinomimetic effects of kaempferitrin on glycaemia and on 14C-glucose uptake in rat soleus muscle. Chem. Biol. Interact. 149, 89–96.

    CAS  Article  Google Scholar 

  16. 16.

    Khalil, N. M., Pepato, M. T., Brunetti, I. L. (2008) Free radical scavenging profile and myeloperoxidase inhibition of extracts from antidiabetic plants: Bauhinia forficata and Cissus sicyoides. Biol. Res. 41, 165–171.

    Article  Google Scholar 

  17. 17.

    Lino, C. S., Diógenes, J. P. L., Pereira, B. A., Faria, R. A. P. G., Andrade Neto, M., Alves, R. S., Queiroz, M. G. R., Souza, F. C. F., Viana, G. S. B. (2004) Antidiabetic activity of Bauhinia forficata extracts in alloxan-diabetic rats. Biol. Pharm. Bull. 27, 125–127.

    CAS  Article  Google Scholar 

  18. 18.

    Ohkawa, H., Ohishi, N., Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351–358.

    CAS  Article  Google Scholar 

  19. 19.

    Pepato, M. T., Keller, E. H., Baviera, A. M., Kettelhut, I. C., Vendramini, R. C., Brunetti, I. L. (2002) Anti-diabetic activity of Bauhinia forficata decoction in streptozotocin-diabetic rats. J. Ethno-pharmacol. 81, 191–197.

    CAS  Article  Google Scholar 

  20. 20.

    Petry, R. D., De Souza, K. C. B., Bassani, V. L., Petrovick, P. R., Gonzalez-Ortega, G. (1998) Doseamento do teor de flavonóides totais em extratos em extratos hidroalcoolicos de Passiflora alata Dryander (maracujá). Rev. Bras. Farm. 79, 7–10.

    CAS  Google Scholar 

  21. 21.

    Pinheiro, T. S. D. B., Johansson, L. A. P., Pizzolatti, M. G., Biavatti, M. W. (2006) Comparative assessment of kaempferitrin from medicinal extracts of Bauhinia forficata Link. J. Pharm. Biomed Anal. 41, 431–436.

    CAS  Article  Google Scholar 

  22. 22.

    Pizzolatti, M. G., Cunha Jr., A., Szpoganicz, B., De Souza, E., Braz-Filho, R., Schripsema, J. (2003) Flavonóides glicosilados das folhas e flores de Bauhinia forficata (Leguminosae). Quim. Nov. 26, 466–469.

    CAS  Article  Google Scholar 

  23. 23.

    Rajkapoor, B., Jayakar, B., Murugesh, N. (2003) Antitumour activity of Bauhinia variegata on Dalton’s ascitic lymphoma. J. Ethnopharmacol. 89, 107–109.

    CAS  Article  Google Scholar 

  24. 24.

    Rao, Y. K., Geethangili, M., Fang, S., Tzeng, Y. (2007) Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: A comparative study. Food Chem. Toxicol. 45, 1770–1776.

    CAS  Article  Google Scholar 

  25. 25.

    Rice-Evans, C. A., Miller, N. J., Paganga, G. (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20, 933–956.

    CAS  Article  Google Scholar 

  26. 26.

    Silva, C. G., Herdeiro, R. S., Mathias, C. J., Panek, A. D., Silveira, C. S., Rodrigues, V. P., Rennó, M. N., Falcão, D. Q., Cerqueira, D. M., Minto, A. B. M., Nogueira, F. L. P., Quaresma, C. H., Silva, J. F. M., Menezes, F. S., Eleutherio, E. C. A. (2005) Evaluation of antioxidant activity of Brazilian plants. Pharmacol. Res. 52, 229–233.

    CAS  Article  Google Scholar 

  27. 27.

    Silva, E. G., Behr, G. A., Zanoto-Filho, A., Lorenzi, R., Pasquali, M. A. B., Ravazolo, L. G., Bordignon Jr, C. L., Silva, F. A., Aboy, A. L., Bassani, V. L., Henriques, A. T., Reginatto, F. H., Dal-Pizzol, F., Moreira, C. F. (2007) Antioxidant activities and free radical scavenging potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) extracts linked to their polyphenol content. Biol. Pharm. Bull. 30, 1488–1496.

    Article  Google Scholar 

  28. 28.

    Temiz-Arpaci, O., Coban, T., Tekiner-Gulbas, B., Can-Eke, B., Yildiz, I., Aki-Sener, E., Yalcin, I., Iscan, M. (2006) A study on the antioxidant activities of some new benzazole derivatives. Acta Biol. Hung. 57, 201–209.

    CAS  Article  Google Scholar 

  29. 29.

    Urquiaga, I., Leighton, F. (2000) Plant polyphenol antioxidants and oxidative stress. Biol. Res. 33, 55–64.

    CAS  Article  Google Scholar 

  30. 30.

    Vasconcelos, F., Sampaio, S. V., Garófalo, M. A. R., Guimarães, L. F. L., Giglio, J. R., Arantes, E. C. (2004) Insulin-like effects of Bauhinia forficata aqueous extract upon Tityus serrulatus scorpion envenoming. J. Ethnopharmacol. 95, 385–392.

    Article  Google Scholar 

  31. 31.

    Vaz, A. M. S. F. (2010) Bauhinia. In: Lista de Espécies da Flora do Brasil. Jardim Botânico, Rio de Janeiro, Brazil.

    Google Scholar 

  32. 32.

    Volpato, G. T., Damasceno, D. C., Rudge, M. V. C., Padovani, C. R., Calderon, I. M. P. (2008) Effect of Bauhinia forficata aqueous extract on the maternal-fetal outcome and oxidative stress biomarkers of streptozotocin-induced diabetic rats. J. Ethnopharmacol. 116, 131–137.

    CAS  Article  Google Scholar 

  33. 33.

    Yadava, R. N., Reddy, V. M. S. (2001) New flavone glycoside, 5-hydroxy 7,3’,4’,5’-tetra-methoxyfla-vone 5-O-β-D-xylopyranosyl-(1→2)-α-L-rhamnopyranoside from Bauhinia variegata Linn. J. Asian Nat. Prod. Res. 3, 341–346.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. Mendez.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Sayago, C.T.M., de Camargo, V.B., Barbosa, F. et al. Chemical Composition and in vitro Antioxidant Activity of Hydro-Ethanolic Extracts from Bauhinia forficata subsp. Pruinosa and B. variegata. BIOLOGIA FUTURA 64, 21–33 (2013). https://doi.org/10.1556/ABiol.64.2013.1.3

Download citation

Keywords

  • Bauhinia species
  • flavonoids
  • HPLC analysis
  • antioxidant activity