In vitro Aversion Conditioning in Lymnaea

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Abstract

In an in vitro semi-intact Lymnaea preparation we were successful in using a training procedure to bring about Conditioning Taste Aversion (CTA). Following paired presentation of the CS (sucrose) and US (tactile), the CS no longer elicits feeding. We can use sucrose to the lips in the semi-intact preparation as the CS; while we use direct current injection to depolarize RPeD11 as the US. Following pairing of these stimuli, the CS no longer elicits fictive feeding. We can determine the changes in synaptic input to neurons that play key roles in controlling feeding behavior.

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Correspondence to M. Sakakibara.

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Presented during the 12th ISIN Symposium on Invertebrate Neurobiology, August 31–September 4, 2011, Tihany, Hungary.

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Sunada, H., Lukowiak, K. & Sakakibara, M. In vitro Aversion Conditioning in Lymnaea. BIOLOGIA FUTURA 63, 190–193 (2012). https://doi.org/10.1556/ABiol.63.2012.Suppl.2.24

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Keywords

  • Taste avoidance conditioning
  • cerebral giant cell
  • right pedal dorsal 11 neuron
  • in vitro conditioning