Repeated Simultaneous Cortical Electrophysiological and Behavioral Recording in Rats Exposed to Manganese-Containing Nanoparticles

Abstract

Male Wistar rats wearing chronically implanted cortical electrodes were exposed to Mn-containing nano-particles via the airways for 8 weeks following a 2-week pre-exposure period. The rats’ cortical electrical activity and open field motility was recorded simultaneously, in weekly repetitions. It was supposed that this technique can provide better insight in the development of Mn-induced CNS damage. Decreased motility (less distance covered, longer periods of immobility) and increased total power of cortical electrical activity developed in parallel in the first 4–5 weeks of treatment but showed little change afterwards. Both the behavioral and the electrophysiological effect were in fair correlation with the rats’ internal Mn exposure determined from brain samples. The results confirmed the non-linear dose- and time-dependence of Mn effects suggested by previous studies. Repeated simultaneous behavioral and electrophysiological recording during a longer treatment with neurotoxic metals (or other xenobiotics) seems to be a promising method.

References

  1. 1.

    Antonini, J. M., Lewis, A. B., Roberts, J. R., Whaley, D. A. (2003) Pulmonary effects of welding fumes: review of worker and experimental animal studies. Am. J. Ind. Med 43, 350–360.

    CAS  Article  Google Scholar 

  2. 2.

    Bader, M., Dietz, M. C., Hiring, A., Triebig, G. (1999) Biomonitoring of manganese in blood, urine and axillary hair following low-dose exposure during the manufacture of dry cell batteries. Int. Arch. Occup. Environ. Health 72, 521–527.

    CAS  Article  Google Scholar 

  3. 3.

    Bowler, R., Koller, W., Schultz, P. E. (2006) Parkinsonism due to manganism in a welder: Neurological and neuropsychological sequelae. Neurotoxicology 27, 327–332.

    CAS  Article  Google Scholar 

  4. 4.

    Calderon-Garciduenas, L., Azzarelli, B., Acuna, H. Garcia, R., Gambling, T. M., Osnaya, N., Monroy S., Tizapantzi M. D. R., Carson, J. L., Villarreal-Calderon, A., Rewcastle, B. (2002) Air pollution and brain damage. Toxicol. Pathol. 3, 373–389.

    Article  Google Scholar 

  5. 5.

    Calne, D. B., Chu, N. S., Huang, C. C., Lu, C. S., Olanow, W. (1994) Manganism and idiopathic Parkinsonism: Similarities and differences. Neurology 44, 1583–1586.

    CAS  Article  Google Scholar 

  6. 6.

    Couper, J. (1837) On the effects of black oxide of manganese when inhaled into the lungs. Br. Ann Med. Pharm. Vit. Stat. Gen. Sci. 1, 41–42.

    Google Scholar 

  7. 7.

    Erikson, K. M., Aschner, M. (2003) Manganese neurotoxicity and glutamate-GABA interaction. Neurochem. Int. 43, 475–180.

    CAS  Article  Google Scholar 

  8. 8.

    Gwiazda, R., Lucchini, R., Smith, D. (2007) Adequacy and consistency of animal studies to evaluate the neurotoxicology of chronic low-level manganese exposure in humans. J. Toxicol. Environ. Health 70, 594–605.

    CAS  Article  Google Scholar 

  9. 9.

    Halatek, T., Sinczuk-Walczak, H., Szymcsak, M., Rydzynski, K. (2005) Neurological and respiratory symptoms in shipyard welders exposed to manganase. Int. J. Occup. Med Environ. Health 18, 265–274.

    PubMed  Google Scholar 

  10. 10.

    He, S. C., Niu, Q. (2004) Subclinical neurophysiological effects of manganese in welding workers. Int. J. Immunopathol. Pharmacol. 17, 11–16.

    CAS  Article  Google Scholar 

  11. 11.

    ICRP (1994) Human respiratory tract model for radiological protection. A report of a task group of the ICRP. Annals of the International Commission on Radiation Protection, ICRP Publication 66, Pergamon Press, Oxford

    Google Scholar 

  12. 12.

    Mate, Zs., Szabo, A., Oszlanczi, G., Papp, A. (2011) Modelling per os Mn exposure and examining its functional nervous system effects in rats. Egeszsegtudomdny 55, 71–81 (in Hungarian).

    Google Scholar 

  13. 13.

    Mena, I., Marin, O., Fuenzalida, S., Cotzias, G. C. (1967) Chronic manganese poisoning: clinical picture and manganese turnover. Neurology 17, 128–136.

    CAS  Article  Google Scholar 

  14. 14.

    Normandin, L., Beaupre, L. A., Salehi, F., St-Pierre, A., Kennedy, G., Mergler, D., Butterworth, R. F., Philippe, S., Zayed, J. (2004) Manganese distribution in the brain and neurobehavioral changes following inhalation exposure of rats to three chemical forms of manganese. Neurotoxicology 25, 411–433.

    Article  Google Scholar 

  15. 15.

    Oberdorster, G., Oberdorster, E., Oberdorster, J. (2005) Nanotoxicology: An emerging discipline evolving from studies of ultrafine particles. Environ. Health Persp. 7, 823–839.

    Article  Google Scholar 

  16. 16.

    Oszlanczi, G., Vezer, T., Sarkozi, L., Horvath, E., Konya, Z., Papp, A. (2010) Functional neurotoxicity of Mn-containing nanoparticles in rats. Ecotoxicol. Environ. Sqf. 73, 2004–2009.

    CAS  Article  Google Scholar 

  17. 17.

    Santamaria, A. B., Cushing, C. A., Antonini, J. M., Finley, B. L., Mowat, F. S. (2007) State-of-the-science review: Does manganese exposure during welding pose a neurological risk? J. Toxicol. Environ. Health, PartB 10, 417–165.

    CAS  Article  Google Scholar 

  18. 18.

    Saric, M., Markicevic, A., Hrustic, O. (1977) Occupational exposure to manganese. Br. J. Ind. Med 34, 114–118.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Sinczuk-Walczak, H., Jakubowski, M., Matczak, W. (2001) Neurological and neurophysiological examinations of workers occupationally exposed to manganese. Int. J. Occup. Med. Environ. Health 14, 329–337.

    CAS  PubMed  Google Scholar 

  20. 20.

    Sjogren, B., Iregren, A., Freeh, W., Hagman, M., Johansson, L., Tesarz, M., Wennberg, A. (1996) Effects of the nervous system among welders exposed to aluminium and manganese. Occup. Environ Med. 53, 32–10.

    CAS  Article  Google Scholar 

  21. 21.

    Takacs, Sz., Papp, A. (2010) Effects of antiepileptics and an anesthetic on basal cortical activity and spontaneous motility in an epilepsy-prone rat strain. Acta Physiol. Hung. 97, 480–181.

    Google Scholar 

  22. 22.

    Takeda, A., Sawashita, J., Okada, S. (1995) Biological half-lives of zinc and manganese in rat brain. Brain Res. 695, 53–58.

    CAS  Article  Google Scholar 

  23. 23.

    Tapin, D., Kennedy, G., Lambert, J., Zayed, J. (2006) Bioaccumulation and locomotor effects of manganese sulphate in Sprague-Dawley rats following subchronic (90 days) inhalation exposure. Toxicol. Appl. Pharmacol. 211, 166–174.

    CAS  Article  Google Scholar 

  24. 24.

    Vezer, T., Kurunczi, A., Naray, M., Papp, A., Nagymajtenyi, L. (2007) Behavioral effects of sub-chronic inorganic manganese exposure in rats. Amer J. Ind. Med 50, 841–852.

    CAS  Article  Google Scholar 

  25. 25.

    Vezer, T., Papp, A., Hoyk, Z., Varga, C., Naray, M., Nagymajtenyi, L. (2005) Behavioral and neuro-toxicological effects of subchronic manganese exposure in rats. Env. Toxicol. Pharmacol. 19, 797–810.

    CAS  Article  Google Scholar 

  26. 26.

    Young, T., Myers, J. E., Thompson, M. L. (2005) The nervous system effects of occupational exposure to manganese - measured as respirable dust - in a South African manganese smelter. Neurotoxicology 26, 993–1000.

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to Mr. József Koszta and Ms. Edit Pálinkás at the laboratory of the MOL Hungarian Oil and Gas Company for the manganese level determinations.

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Correspondence to A. Papp.

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Takács, S., Szabó, A., Oszlánczi, G. et al. Repeated Simultaneous Cortical Electrophysiological and Behavioral Recording in Rats Exposed to Manganese-Containing Nanoparticles. BIOLOGIA FUTURA 63, 426–440 (2012). https://doi.org/10.1556/ABiol.63.2012.4.2

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Keywords

  • Manganese
  • rat
  • cortical electrical activity
  • open field motility
  • chronic recording