The Optimization of Regeneration Tissue Culture System of Three Chilli Peppers Cultivars Based on the Uniform Design and the Mathematical Model Equation
Using uniform random design optimization and the mathematical model equation we optimized the regeneration tissue culture system of the chilli pepper. An efficient and detailed plant reproducible protocol in vitro has been established using different expiants and induction media for three chilli pepper cul-tivars. The result displayed that the seedlings at the curved hypocotyl stage were the best choice to prepare for expiants, the genotype of expiants affected shoot buds induction frequency and number of shoot buds per expiant, and the cotyledon expiant was more responsive than hypocotyl expiant. The optimal media for maximum shoot initiation and regeneration and the optimal elongation medium were obtained. For Capsicum annuum var. annuum (cv. Xinsu), Capsicum annuum var. annuum (cv. Neimengchifeng) and Capsicum frutescens (cv. Xingfu), the induction rates were 99.17%, 97.50 and 96.11%, respectively; the elongation rates of shoot buds were 86.67%, 85.19% and 82.96%, respectively. The MS medium with 0.57 μM IAA and 0.69 μM NAA is the best choice for root induction. The frequency of their root emergence was 95.00-98.33%. Regenerated chilli peppers were successfully acclimatized and cultivated with 100% survival. This work will help to improve multiplication process and the genotype of chilli pepper, and may have commercial impact.
KeywordsChilli pepper mathematical model equation regeneration tissue culture uniform random design optimization
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