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Acta Biologica Hungarica

, Volume 62, Issue 3, pp 235–243 | Cite as

in Vitro and in Vivo Evaluation of Antioxidant Activity of Trichosanthes Cucumerina Aerial Parts

  • Menuka ArawwawalaEmail author
  • Ira Thabrew
  • Lakshmi Arambewela
Article

Abstract

The present study was conducted to determine whether aerial parts of Trichosanthes cucumerina extracts can exert significant antioxidant activity. The antioxidant activity of a. hot water extract (HWE) and a. cold ethanolic extract (CEE) of T. cucumerina aerial parts was evaluated by assessing its (a) radical scavenging ability and prevention effect of lipid peroxidation in vitro, and (b) effects on lipid peroxidation and antioxidant enzyme activities, in vivo.

In vitro antioxidant assays (DPPH, TBARS and carotene-linoleic acid assays) clearly demonstrated the antioxidant potential of HWE and CEE. Moreover, HWE increased SOD: by 91.2% and GPyby 104.4% while CEE increased SOD: by 115.5% and GPyby 96.4%) in CCl4-induced rats. Treatments with HWE and CEE prevented the accumulation of lipid peroxidation products by 30.5% and 33.8%, respectively, in liver tissues compared to the rats exposed only to CC14. In conclusion, the present investigation demonstrates for the first time that components in T. cucumerina aerial parts can exert significant antioxidant activity in vivo and in vitro.

Keywords

Trichosanthes cucumerina free radical scavenger lipid peroxidation SOD and GPX levels 

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Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Menuka Arawwawala
    • 1
    Email author
  • Ira Thabrew
    • 2
  • Lakshmi Arambewela
    • 1
  1. 1.Industrial Technology InstituteBauddhaloka MawathaColomboSri Lanka
  2. 2.Institute of Biochemistry, Molecular Biology and BiotechnologyUniversity of ColomboColomboSri Lanka

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