in Vitro and in Vivo Evaluation of Antioxidant Activity of Trichosanthes Cucumerina Aerial Parts


The present study was conducted to determine whether aerial parts of Trichosanthes cucumerina extracts can exert significant antioxidant activity. The antioxidant activity of a. hot water extract (HWE) and a. cold ethanolic extract (CEE) of T. cucumerina aerial parts was evaluated by assessing its (a) radical scavenging ability and prevention effect of lipid peroxidation in vitro, and (b) effects on lipid peroxidation and antioxidant enzyme activities, in vivo.

In vitro antioxidant assays (DPPH, TBARS and carotene-linoleic acid assays) clearly demonstrated the antioxidant potential of HWE and CEE. Moreover, HWE increased SOD: by 91.2% and GPyby 104.4% while CEE increased SOD: by 115.5% and GPyby 96.4%) in CCl4-induced rats. Treatments with HWE and CEE prevented the accumulation of lipid peroxidation products by 30.5% and 33.8%, respectively, in liver tissues compared to the rats exposed only to CC14. In conclusion, the present investigation demonstrates for the first time that components in T. cucumerina aerial parts can exert significant antioxidant activity in vivo and in vitro.


  1. 1.

    Adebooye, O. C. (2007) Activity and components of antioxidants in the fruit pulp of snake tomato (Trichosanthes cucumerina L.). Elec. J. Environ. Agric. Food Chem. 6, 1912–1920.

    CAS  Google Scholar 

  2. 2.

    Arawwawala, M., Thabrew, I., Arambewela, L. (2009) Antidiabetic activity of Trichosanthes cucumerina in normal and streptozotocin - induced diabetic rats. Int. J. Biol. Chem. Sci. 3, 287–296.

    Google Scholar 

  3. 3.

    Arawwawala, L. D. A. M., Thabrew, M. I., Arambewela, L. S. R. (2010) Gastroprotective activity of Trichosanthes cucumerina in rats. J. Ethnopharmacol. 127, 750–754.

    CAS  Article  Google Scholar 

  4. 4.

    Coppen, P. P. (1983) The use of antioxidants. In: Allen, J. C., Hamilton, R. J. (eds) Rancidity inFoods. Publication of Applied Science Publishers, U.K., pp. 67–88.

    Google Scholar 

  5. 5.

    Dani, C., Pasquali, M. A. B., Oliveira, M. R., Umezu, F. M., Salvador, M., Henriques, J. A. P., Moreira, J. C. F. (2008) Protective effects of purple grape juice on carbon tetrachloride-induced oxidative stress in brains of adult wistar rats. J. Med Food 11, 55–61.

    CAS  Article  Google Scholar 

  6. 6.

    Dorman, H. J. D., Deans, S. G., Noble, R. C., Surai, P. (1995) Evaluation in vitro of plant essential oils as natural antioxidants. J. Essent. Oil Res. 7, 645–651.

    CAS  Article  Google Scholar 

  7. 7.

    Espin, J. C., Soler-Rivas, C., Wichers, H. J., Garcia-Viguera, C. (2000) Anthocyanin-based natural colorants: a. new source of antiradical activity for foodstuff. J. Agric. Food Chem. 48, 1588–1592.

    CAS  Article  Google Scholar 

  8. 8.

    Halliwell, B., Cross, C. E. (1994) Oxygen-derived species: their relation to human disease and environmental stress. Environ. Health Perspect. 102, 5–12.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Jang, H. D., Chang, K. S., Huang, Y. S., Hsu, C. L., Lee, S. H., Su, M. S. (2007) Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants. Food Chem. 103, 749–756.

    CAS  Article  Google Scholar 

  10. 10.

    Jayaprakasha, G. K., Singh, R. P., Sakariah, K. K. (2001) Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73, 285–290.

    CAS  Article  Google Scholar 

  11. 11.

    Jayaweera, D. M. A. (1980) Medicinal Plants (Indigenous and Exotic) Used in Ceylon, Part 2. Publication of the National Science Council of Sri Lanka, Colombo.

    Google Scholar 

  12. 12.

    Jeon, T. I., Hwang, S. G., Park, N. G., Jung, Y. R., Shin, S. I., Choi, S. D., Park, D. K. (2003) Antioxidant effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats. Toxicology 187, 67–73.

    CAS  Article  Google Scholar 

  13. 13.

    Kar, A., Choudhary, B. K., Bandyopadhyay, N. G. (2003) Comparative evaluation of hypoglycemic activity of some Indian medicinal plants in alloxan diabetic rats. J. Ethnopharmacol. 84, 105–108.

    Article  Google Scholar 

  14. 14.

    Kolte, R. M., Bisan, V. V., Jangde, C. R., Bhalerao, A. A. (1996-1997) Anti-inflammatory activity of root tubers of Trichosanthes cucumerina (Linn) in mouse’s hind paw oedema induced by carrageenin. Indian J. Indigenous Med. 18, 117–121.

    Google Scholar 

  15. 15.

    Kongtun, S., Jiratchariyakul, W., Kummalue, T., Tanariya, P., Kunnachak, S., Frahm, A. W. (2009) Cytotoxic properties of root extract and fruit juice of Trichosanthes cucumerina. Planta Medica 75, 839–842.

    CAS  Article  Google Scholar 

  16. 16.

    Kumar, S. S., Kumar, B. R., Mohan, G. K. (2009) Hepatoprotective effect of Trichosanthes cucumerina var. cucumerina L. on carbon tetrachloride induced liver damage in rats. J. Ethnopharmacol. 123, 347–350.

    Google Scholar 

  17. 17.

    McGregor, D., Lang, M. (1996) Carbon tetrachloride: genetic effects and other models of action. Mut. Res. 366, 181–195.

    CAS  Google Scholar 

  18. 18.

    Modder, W. W. D., Amarakoon, A. M. T. (2002) Antioxidant activity. In: Tea and Health. Publication of Tea Research Institute, Sri Lanka, pp. 49–64.

    Google Scholar 

  19. 19.

    Mohammadzadeh, S., Sharriatpanahi, M., Hamedi, M., Amanzadeh, Y., Ebrahimi, S. E. S., Ostad, S. N. (2007) Antioxidant power of Iranian propolis extract. Food Chem. 103, 729–733.

    CAS  Article  Google Scholar 

  20. 20.

    Navarro, M. C., Montilla, M. P., Martin, A., Jimenez, J., Utrilla, M. P. (1993) Free radical scavenger and antihepato toxic activity of Rosmarinus tomentosus. Planta Medica 59, 312–314.

    Article  Google Scholar 

  21. 21.

    Paget, G. E., Barnes, J. M. (1996) Toxicity tests. In: Lawrence, D. R., Bacharach, A. L. (eds) Evaluation of Drug Activities: Pharmacometrics (Vol. 1). Publication of Academic Press, New York.

    Google Scholar 

  22. 22.

    Perchellet, J. P., Perchellet, E. M. (1989) Antioxidants and multistage carcinogenesis in mouse skin. Free Radic. Biol. Med. 7, 377–408.

    CAS  Article  Google Scholar 

  23. 23.

    Summerfield, E. W., Tappel, A. L. (1983) Determination of fluorescence quenching of the environment of DNA cross-links made by malondialdehyde. Biochimica et Biophysica Acta 740, 185–189.

    CAS  Article  Google Scholar 

  24. 24.

    Yaping, Z., Suping, Q., Wenli, Y., Zheng, X., Hong, S., Side, Y., Dapu, W. (2002) Antioxidant activity of lycopene extracted from tomato paste towards trichloromethyl peroxyl radical CC13O2. Food Chem. 77, 209–212.

    Article  Google Scholar 

  25. 25.

    Zheng, W., Wang, S. Y. (2001) Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 49, 5165–5170.

    CAS  Article  Google Scholar 

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Correspondence to Menuka Arawwawala.

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Arawwawala, M., Thabrew, I. & Arambewela, L. in Vitro and in Vivo Evaluation of Antioxidant Activity of Trichosanthes Cucumerina Aerial Parts. BIOLOGIA FUTURA 62, 235–243 (2011).

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  • Trichosanthes cucumerina
  • free radical scavenger
  • lipid peroxidation
  • SOD and GPX levels