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Acta Biologica Hungarica

, Volume 62, Issue 2, pp 204–210 | Cite as

Characterization of Stress Processes of Phaffia Rhodozyma Stress-Resistant Mutant

  • E. Horváth
  • G. Papp
  • Z. Gazdag
  • J. Belágyi
  • Á. Blaskó
  • J. Deli
  • Cs. Vágvölgyi
  • M. PestiEmail author
Article

Abstract

A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

Keywords

Phaffia rhodozyma carotenoid oxidative stress EPR GSH 

Abbreviations

Cr(VI)

chromate

DHR123

dihydrorhodamine 123

ET

ethidium

EPR

electron paramagnetic resonance

GSH

glutathione

GSSG

oxidized glutathione

GR

glutathione reductase

H2O2

hydrogen peroxide

MD

menadione

MIC

minimal inhibitory concentration

OH

hydroxyl radical

O2·

superoxide radical

ROS

reactive oxygen species

SOD

superoxide dismutase

t-BOOH

tert-butylhydroperoxide

PBN

N-tert-butyl-phenyl nitron

P. rhodozyma

Phaffia rhodozyma

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • E. Horváth
    • 1
  • G. Papp
    • 1
  • Z. Gazdag
    • 1
  • J. Belágyi
    • 2
  • Á. Blaskó
    • 1
  • J. Deli
    • 3
  • Cs. Vágvölgyi
    • 4
  • M. Pesti
    • 1
    Email author
  1. 1.Department of General and Environmental Microbiology, Faculty of SciencesUniversity of PécsPécsHungary
  2. 2.Institute of BiophysicsUniversity of PécsPécsHungary
  3. 3.Department of Biochemistry and Medical Chemistry, Faculty of MedicineUniversity of PécsPécsHungary
  4. 4.Department of Microbiology, Faculty of Science and InformaticsUniversity of SzegedSzegedHungary

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