Advertisement

Acta Biologica Hungarica

, Volume 62, Issue 1, pp 45–56 | Cite as

Assessment of Factors Affecting Micropropagation and Ex Vitro Acclimatization of Nyctanthes Arbor-Tristis L.

  • Anushi Arjumend Jahan
  • M. AnisEmail author
  • I. M. Aref
Article

Abstract

Rapid differentiation of multiple shoots was observed in 94% of nodal explants of one year old Nyctanthes arbor-tristis L. plants. Shoot bud induction and multiplication took place on Murashige and Skoog (MS) medium supplemented with two cytokinins, i.e. Benzyladenine (BA) or Kinetin (Kn) either alone or in combination with different auxins, indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) or α-naphthalene acetic acid (NAA). Between different media, pH levels and growth regulators tried, the optimum condition for maximum regenerative response was obtained on MS?+Kn (2.5 μM)+ N AA (0.5 μM) media at 5.8 pH, forming cultures with 23.26±0.89 number of shoots and 6.36±0.80 cm shoot length after 8 weeks of culture. Histological sections confirmed the formation of multiple buds from nodal explants. Rooting was achieved ex vitro by dipping the basal ends of microshoots in 200 μM IBA for 30 min followed by their transplantation in sterile soilrite. The plantlets with well-developed shoot and root system were successfully established in garden soil and grown outside in a greenhouse with a 80% survival rate.

Keywords

Clonal propagation ex vitro rooting nodal segment Nyctanthes arbor-tristis Oleaceae 

Abbreviations

BA

6-Benzyladenine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

Kn

Kinetin (6-furfurylaminopurine)

NAA

a-naphthalene acetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ahmad, N., Anis, M. (2007) Rapid clonal propagation of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agroforest. Syst. 71, 195–200.CrossRefGoogle Scholar
  2. 2.
    Amo-Marco, J. B., Ibanez, M. R. (1998) Micropropagation of Limonium cavanillesii Erben, a threatened statice, from inflorescence stems. Plant Growth Reg. 24, 49–54.CrossRefGoogle Scholar
  3. 3.
    Anis, M., Husain, M. K., Shahzad, A. (2005) In vitro plantlet regeneration of Pterocarpus marsupium (Roxb.), an endangered leguminous tree. Curr. Sci. 88, 861–863.Google Scholar
  4. 4.
    Anonymous (2001) The Wealth of India: A Dictionary of Indian Raw Materials and Industrial Products. Vol. VII. N-Pe. Publications and Information Directorate CSIR, New Delhi, pp. 69–70.Google Scholar
  5. 5.
    Bhatt, I. D., Dhar, U. (2004) Factors controlling micropropagation of M. esculenta buch-Ham. Ex. D. Don: a high value wild edible of Kumaon Himalaya. Afri. J. Biotech. 3, 534–540.CrossRefGoogle Scholar
  6. 6.
    Bhattacharya, S., Das, B., Ghose, T. K., Bhattacharya, S. (1999) Investigation on seed germination of Nyctanthes arbor-tristis (Oleaceae) in relation to the total phenol content. Sci. and Technol. 27, 321–327.Google Scholar
  7. 7.
    Borthakur, M., Hazarika, J., Singh, S. R. (1999) A protocol for micropropagation of Alpinia galanga. Plant Cell Tiss. Organ Cult. 55, 231–233.CrossRefGoogle Scholar
  8. 8.
    Brown, D. C. W., Leung, D. W. M., Thorpe, T. A. (1979) Osmotic requirement for shoot formation in tobacco callus. Physiol. Plant. 46, 36–41.CrossRefGoogle Scholar
  9. 9.
    Chaudhuri, R. K., Jha, T. B. (2008) Conservation and production of Ipecac (Cephalis ipecacuanha Rich.) plants from long term cultures. Plant Tiss. Cult. Biotech. 18, 157–164.CrossRefGoogle Scholar
  10. 10.
    Dhar, U., Upreti, J. (1999) In vitro regeneration of mature liana (Bauhinia vahlii Wight and Arnott). Plant Cell Rep. 18, 664–669.CrossRefGoogle Scholar
  11. 11.
    Faisal, M., Ahmad, N., Anis, M. (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss. Organ Cult. 80, 187–190.CrossRefGoogle Scholar
  12. 12.
    Faisal, M., Siddique, I., Anis, M. (2006) An efficient plant regeneration system for Mucuna pruriens L. using cotyledonary node explants. In Vitro Cell. Dev. Biol.-Plant 42, 59–64.CrossRefGoogle Scholar
  13. 13.
    Gamborg, O. L., Miller, R. A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soyabean root cells. Exp. Cell Res. 50, 151–158.CrossRefGoogle Scholar
  14. 14.
    Gautam, V. K., Nanda, K., Gupta, S. C. (1993) Development of shoots and roots in anther-derived callus of Azadirachta indica A. Juss.–a medicinal tree. Plant Cell Tiss. Organ Cult. 34, 13–18.CrossRefGoogle Scholar
  15. 15.
    Huda, K. M. K., Bhuiyan, M. S. R., Zeba, N., Banu, S. A., Mahmud, F., Khatun, A. (2009) Effects of FeSO4 and pH on shoot regeneration from the cotyledonary node explants of Tossa jute. Plant Omics J. 2, 190–196.Google Scholar
  16. 16.
    Hussein, S., Ibrahim, R., Kiong, A. L. P., Fadzillah, N. M., Daud, S. K. (2005) Multiple shoots formation of an important tropical medicinal plant, Eurycoma longifolia Jack. Plant Biotechnol. 22, 349–351.CrossRefGoogle Scholar
  17. 17.
    Iyer, R. I., Mathuram, V., Gopinath, P. M. (1998) Establishment of callus cultures of Nyctanthes arbor-tristis from juvenile explants and detection of secondary metabolites in the callus. Curr. Sci. 74, 243–246.Google Scholar
  18. 18.
    Joshi, M., Dhar, U. (2003) In vitro propagation of Saussurea aobvallata (D.C.) Edgew–an endangered ethnoreligeous medicinal herb of Himalaya. Plant Cell Rep. 21, 933–939.CrossRefGoogle Scholar
  19. 19.
    Kamstaityte, D., Stanys, V. (2004) Micropropagation of onion (Allium cepa L.). Acta Univers. Latv. Biol. 676, 173–176.Google Scholar
  20. 20.
    Kim, M. S., Klopfenstein, N. B., Cregg, B. M. (1998) In vitro and ex vitro rooting of micropropagated shoots using three green ash (Fraxinus pennsylvanica) clones. New Fores. 16, 43–57.CrossRefGoogle Scholar
  21. 21.
    Lameira, O. A., Pinto, J. E. B. P. (2006) In vitro propagation of Cordia verbenaceae L. (Boraginaceae). Rev. Bras. Plant Med. Botucatu 8, 102–104.Google Scholar
  22. 22.
    Lloyd, G. B., McCown, B. H. (1980) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Proc. Int. Plant Prop. Soc. 30, 421–427.Google Scholar
  23. 23.
    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
  24. 24.
    Nair, L. G., Seeni, S. (2003) In vitro multiplication of Calophyllum apetalum (Clusiaceae), an endemic medicinal tree of the Western Ghats. Plant Cell Tiss. Organ Cult. 75, 169–174.CrossRefGoogle Scholar
  25. 25.
    Nandwani, D. (1994) Clonal propagation of Myrica esculenta (Box-berry) A fruit bearing tree of north-east India. Gartenbauwissenschaft (Horticultural Science) 59, 264–267.Google Scholar
  26. 26.
    Nanu, R., Raghuveer, I., Chitme, H. R., Chandra, R. (2008) Antidiabetic activity of Nyctanthes arbortristis. Pharmacog. Mag. 4, 335–340.Google Scholar
  27. 27.
    Phillips, G. C., Collins, G. B. (1979) In vitro tissue culture of selected legumes and plant regeneration from callus of red clover. Crop Sci. 19, 59–64.CrossRefGoogle Scholar
  28. 28.
    Purohit, S. D., Singhvi, A. (1998) Micropropagation of Achras sapota through enhanced axillary branching. Sci. Hort. 76, 219–229.CrossRefGoogle Scholar
  29. 29.
    Rout, G. R., Mahato, A., Senapati, S. K. (2007) In vitro clonal propagation of Nyctanthes arbortristis L.–a medicinal tree. Hort. Sci. 34, 84–89.Google Scholar
  30. 30.
    Rout, G. R., Mahato, A., Senapati, S. K. (2008) In vitro clonal propagation of Nyctanthes arbortristis L. Biol. Plant. 52, 521–524.CrossRefGoogle Scholar
  31. 31.
    Selvakkumar, C., Balakrishnan, A., Lakshmi, B. S. (2007) Rapid in vitro micropropagation of Alpinia officinarum Hance. an important medicinal plant through rhizome bud explants. Asia. J. Plant Sci. 6, 1251–1255.CrossRefGoogle Scholar
  32. 32.
    Siddique, I., Anis, M. (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol. Plant. 29, 233–238.CrossRefGoogle Scholar
  33. 33.
    Siddique, I., Anis, M. (2009) Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.): a valuable medicinal tree. New Fores. 37, 53–62.CrossRefGoogle Scholar
  34. 34.
    Siddique, I., Anis, M., Jahan, A. A. (2006) Rapid multiplication of Nyctanthes arbor-tristis L. through in vitro axillary shoot proliferation. World J. Agri. Sci. 2, 188–192.Google Scholar
  35. 35.
    Thapliyal, R. C., Naithani, K. C. (1996) Inhibition of germination in Nyctanthes arbor-tristis (Oleaceae) by pericarp. Seed Sci. Technol. 24, 67.Google Scholar
  36. 36.
    Tomsone, S., Galeniece, A., Akaere, A., Priede, G., Zira, L. (2007) In vitro propagation of Syringa vulgaris L. Cultivars. Biologija 53, 28–31.Google Scholar
  37. 37.
    Xu, J., Wang, Y., Zhang, Y. (2008) Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd. Acta Physiol. Plant. 30, 129–132.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food & Agricultural SciencesKing Saud UniversityRiyadhSaudi Arabia

Personalised recommendations