Acta Biologica Hungarica

, Volume 62, Issue 1, pp 106–111 | Cite as

How Does it Act When Soluble? Critical Evaluation of Mechanism of Galectin-1 Induced T-Cell Apoptosis

  • Andrea Blaskó
  • Roberta Fajka-Boja
  • Gabriela Ion
  • Éva MonostoriEmail author
Open Access
Short Communication


Galectin-1 (Gal-1), a mammalian lectin induces apoptosis of T lymphocytes. Contradictory data have resulted in confusing knowledge regarding mechanism of Gal-1 induced T-cell apoptosis. In this paper we aimed to resolve this controversy by comparing cell death induced by low (1.8 μM, lowGal-1) and high (18 μM, highGal-1) concentration of soluble Gal-1. We show that lowGal-1 and highGal-1 trigger phosphatidylserine exposure, generation of rafts and mitochondrial membrane depolarization. In contrast, lowGal-1 but not highGal-1 is dependent on the presence of p56lck and ZAP70 and activates caspase cascade. The results allow the conclusion that the cell-death mechanism strictly depends on the concentration of Gal-1.


Galectin-1 apoptosis mechanism concentration dependence 


  1. 1.
    Brandt, B., Buchse, T., Abou-Eladab, E.F., Tiedge, M., Krause, E., Jeschke, U., Walzel, H. (2008) Galectin-1 induced activation of the apoptotic death-receptor pathway in human Jurkat T lymphocytes. Histochem. Cell Biol. 129, 599–609.CrossRefGoogle Scholar
  2. 2.
    Chung, C.D., Patel, V.P., Moran, M., Lewis, L.A., Miceli, M.C. (2000) Galectin-1 induces partial TCR zeta-chain phosphorylation and antagonizes processive TCR signal transduction. J. Immunol. 165, 3722–3729.CrossRefGoogle Scholar
  3. 3.
    Cooper, D.N., Barondes, S.H. (1990) Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism. J. Cell Biol. 110, 1681–1691.CrossRefGoogle Scholar
  4. 4.
    Fajka-Boja, R., Szemes, M., Ion, G., Legradi, A., Caron, M., Monostori, E. (2002) Receptor tyrosine phosphatase, CD45 binds galectin-1 but does not mediate its apoptotic signal in T cell lines. Immunol. Lett. 82, 149–154.CrossRefGoogle Scholar
  5. 5.
    Hahn, H.P., Pang, M., He, J., Hernandez, J.D., Yang, R.Y., Li, L.Y., Wang, X., Liu, F.T., Baum, L.G. (2004) Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death. Cell Death Differ. 11, 1277–1286.CrossRefGoogle Scholar
  6. 6.
    He, J., Baum, L.G. (2004) Presentation of galectin-1 by extracellular matrix triggers T cell death. J. Biol. Chem. 279, 4705–4712.CrossRefGoogle Scholar
  7. 7.
    Ion, G., Fajka-Boja, R., Kovacs, F., Szebeni, G., Gombos, I., Czibula, A., Matko, J., Monostori, E. (2006) Acid sphingomyelinase mediated release of ceramide is essential to trigger the mitochondrial pathway of apoptosis by galectin-1. Cell. Signal. 18, 1887–1896.CrossRefGoogle Scholar
  8. 8.
    Ion, G., Fajka-Boja, R., Toth, G.K., Caron, M., Monostori, E. (2005) Role of p56lck and ZAP70-mediated tyrosine phosphorylation in galectin-1-induced cell death. Cell Death Differ. 12, 1145–1147.CrossRefGoogle Scholar
  9. 9.
    Kovacs-Solyom, F., Blasko, A., Fajka-Boja, R., Katona, R.L., Vegh, L., Novak, J., Szebeni, G.J., Krenacs, L., Uher, F., Tubak, V., Kiss, R., Monostori, E. (2010) Mechanism of tumor cell-induced T-cell apoptosis mediated by galectin-1. Immunol. Lett. 127, 108–118.CrossRefGoogle Scholar
  10. 10.
    Matarrese, P., Tinari, A., Mormone, E., Bianco, G.A., Toscano, M.A., Ascione, B., Rabinovich, G.A., Malorni, W. (2005) Galectin-1 sensitizes resting human T lymphocytes to Fas (CD95)-mediated cell death via mitochondrial hyperpolarization, budding, and fission. J. Biol. Chem. 280, 6969–6985.CrossRefGoogle Scholar
  11. 11.
    Nguyen, J.T., Evans, D.P., Galvan, M., Pace, K.E., Leitenberg, D., Bui, T.N., Baum, L.G. (2001) CD45 modulates galectin-1-induced T cell death: regulation by expression of core 2 O-glycans. J. Immunol. 167, 5697–5707.CrossRefGoogle Scholar
  12. 12.
    Pace, K.E., Hahn, H.P., Pang, M., Nguyen, J.T., Baum, L.G. (2000) CD7 delivers a pro-apoptotic signal during galectin-1-induced T cell death. J. Immunol. 165, 2331–2334.CrossRefGoogle Scholar
  13. 13.
    Pace, K.E., Lee, C., Stewart, P.L., Baum, L.G. (1999) Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1. J. Immunol. 163, 3801–3811.PubMedGoogle Scholar
  14. 14.
    Pang, M., He, J., Johnson, P., Baum, L.G. (2009) CD45-mediated fodrin cleavage during galectin-1 T cell death promotes phagocytic clearance of dying cells. J. Immunol. 182, 7001–7008.CrossRefGoogle Scholar
  15. 15.
    Perillo, N.L., Pace, K.E., Seilhamer, J.J., Baum, L.G. (1995) Apoptosis of T cells mediated by galectin-1. Natur. 378, 736–739.CrossRefGoogle Scholar
  16. 16.
    Rabinovich, G.A., Ilarregui, J.M. (2009) Conveying glycan information into T-cell homeostatic programs: a challenging role for galectin-1 in inflammatory and tumor microenvironments. Immunol. Rev. 230, 144–159.CrossRefGoogle Scholar
  17. 17.
    Rabinovich, G.A., Liu, F.T., Hirashima, M., Anderson, A. (2007) An emerging role for galectins in tuning the immune response: lessons from experimental models of inflammatory disease, autoimmunity and cancer. Scand. J. Immunol. 66, 143–158.CrossRefGoogle Scholar
  18. 18.
    Saussez, S., Glinoer, D., Chantrain, G., Pattou, F., Carnaille, B., Andre, S., Gabius, H.J., Laurent, G. (2008) Serum galectin-1 and galectin-3 levels in benign and malignant nodular thyroid disease. Thyroi. 18, 705–712.CrossRefGoogle Scholar
  19. 19.
    Stowell, S.R., Karmakar, S., Stowell, C.J., Dias-Baruffi, M., McEver, R.P., Cummings, R.D. (2007) Human galectin-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells. Bloo. 109, 219–227.CrossRefGoogle Scholar
  20. 20.
    Stowell, S.R., Qian, Y., Karmakar, S., Koyama, N.S., Dias-Baruffi, M., Leffler, H., McEver, R.P., Cummings, R.D. (2008) Differential roles of galectin-1 and galectin-3 in regulating leukocyte viability and cytokine secretion. J. Immunol. 180, 3091–3102.CrossRefGoogle Scholar
  21. 21.
    Sturm, A., Lensch, M., Andre, S., Kaltner, H., Wiedenmann, B., Rosewicz, S., Dignass, A.U., Gabius, H.J. (2004) Human galectin-2: novel inducer of T cell apoptosis with distinct profile of caspase activation. J. Immunol. 173, 3825–3837.CrossRefGoogle Scholar
  22. 22.
    Vespa, G.N., Lewis, L.A., Kozak, K.R., Moran, M., Nguyen, J.T., Baum, L.G., Miceli, M.C. (1999) Galectin-1 specifically modulates TCR signals to enhance TCR apoptosis but inhibit IL-2 production and proliferation. J. Immunol. 162, 799–806.PubMedGoogle Scholar
  23. 23.
    Walzel, H., Schulz, U., Neels, P., Brock, J. (1999) Galectin-1, a natural ligand for the receptor-type protein tyrosine phosphatase CD45. Immunol. Lett. 67, 193–202.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2011

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Authors and Affiliations

  • Andrea Blaskó
    • 1
  • Roberta Fajka-Boja
    • 1
  • Gabriela Ion
    • 1
  • Éva Monostori
    • 1
    Email author
  1. 1.Biological Research CenterHungarian Academy of SciencesSzegedHungary

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