Advertisement

Acta Biologica Hungarica

, Volume 61, Supplement 1, pp 172–188 | Cite as

Seasonal Variation of Leaf Ecophysiological Traits within the Canopy of Quercus petraea (Matt.) Liebl. trees

  • Erzsébet Szőllősi
  • V. Oláh
  • P. Kanalas
  • J. Kis
  • A. Fenyvesi
  • Ilona MészárosEmail author
Article

Abstract

Facing contrasting light regimes during a vegetation season and depending on canopy position, physiological plasticity of leaves is vital for tree species to sustain the optimal ratio between the benefit of carbon assimilation and the costs of photoprotection in a given leaf. We tested the seasonal adjustment of sun and shade leaf photochemistry of sessile oak (Quercus petraea (Matt.) Liebl.) to changing light environments by parallel investigation of the meteorological conditions, photosynthetic pigment content, PSII quantum efficiency and excitation energy quenching. Sun and shade leaves got adapted to their prevailing light regimes till mid of May. High LMA was a favourable trait in avoiding water loss and decreasing photoinhibition in both flushing and sun leaves, while low LMA optimized the light absorbing leaf surface in the lower canopy layer. Partitioning of excitation energy dissipation pathyways that is PSII photochemistry- Y(II), regulated-Y(NPQ) and non-regulated-Y(NO) quenching changed significantly during leaf ontogeny and with the position of leaves in the canopy. At 800 μmol m−2 s−1Y(II) < Y (NO) < Y(NPQ) was characteristic to early developmental stage of leaves from both canopy layers and to mature shade leaves, and Y(NO) < Y (II) < Y(NPQ) to mature sun leaves but the magnitude of Y(NPQ) and violaxanthin cycle activity differed in different canopy positions.

Keywords

Quercus petraea photosynthetic pigments chlorophyll fluorescence sun and shade leaves VAZ (violaxanthin + antheraxanthin + zeaxanthin) 

Abbreviations

CHL

chlorophyll

CAR

carotenoids, Fm, maximum fluorescence in the dark adapted state

Fo

minimum fluorescence in the dark adapted state

Fm

maximum fluorescence in the light adapted state

LMA

leaf mass per area

PPFD

Photosynthetic Photon Flux Density in μmol quanta m−2 s−1

Y(II)

quantum yield of photochemistry in PS II

Y(NO)

quantum yield of non-regulated non-photochemical quenching in PS II

Y(NPQ)

quantum yield of regulated non-photochemical quenching in PS II

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anderson, J. M., Osmond, C. B. (1987) Shade-sun response: compromises between acclimation and photoinhibition. In: Kyle, D. J., Osmond, C. B., Arntzen, C. J. (eds) Photoinhibition. Elsevier, Amsterdam, pp. 1–38.Google Scholar
  2. 2.
    Aranda, I., Pardo, F., Gil, L., Pardos, J. (2004) Anatomical basis of the change in leaf mass per area and nitrogen investment with relative irradiance within the canopy of eight temperate tree species. Acta Oecol. 25, 187–019.CrossRefGoogle Scholar
  3. 3.
    Bartha, D. (1995) Overview of the conditions of Hungarian forests and forest management. IUCN Report, Gland, Switzerland, pp. 24.Google Scholar
  4. 4.
    Bazzaz, F. A., Carlson, R. W. (1982) Photosynthetic acclimation to variability in the light environment of early and late successional plants. Oecologia 54, 313–316.CrossRefGoogle Scholar
  5. 5.
    Brugnoli, E., Scartazza, A., De Tullio, M. C., Monteverdi, M. C., Lauteri, M., Augusti, A. (1998) Zeaxanthin and non-photochemical quenching in sun and shade leaves of C3 and C4 plants. Physiol. Plant. 104, 727–734.CrossRefGoogle Scholar
  6. 6.
    Closa, I., Irigoyen, J. J., Goicoechea, N. (2010) Microclimatic condition determined by stem density influence leaf anatomy and leaf physiology of beech (Fagus sylvatica L.) growing within stands that naturally regenerate from clear-cutting. Trees 24, 1029–1043.CrossRefGoogle Scholar
  7. 7.
    Demmig-Adams, B., Adams, W. W. (1996) Chlorophyll and carotenoid composition in leaves of Euonymus kiautschovicus acclimated to different degrees of light stress in the field. Austr. J. Plant Physiol. 23, 649–659.Google Scholar
  8. 8.
    Demmig-Adams, B., Adams, W. W. (2006) Photoprotection in an ecological context: the remarkable complexity of thermal energy dissipation. Tansley review. New Phytol. 172, 11–21.CrossRefGoogle Scholar
  9. 9.
    Faria, T., García-Plazaola, J. I., Abadía, A., Cerasoli, S., Pereira, J. S., Chaves, M. M. (1996) Diurnal changes in photoprotective mechanisms in leaves of cork oak (Quercus suber) during summer. Tree Physiol. 16, 115–123.CrossRefGoogle Scholar
  10. 10.
    García-Plazaola, J. I., Becerril, J. M., Hernández, A., Niinemets, Ü., Kollist, H. (2004) Acclimation of antioxidant pools to the light environment in a natural forest canopy. New Phytologist 163, 87–97.CrossRefGoogle Scholar
  11. 11.
    Genty, B., Briantais, J. M., Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.CrossRefGoogle Scholar
  12. 12.
    Goodwin T. W., Britton, G. (1988) Distribution and analysis of carotenoids. In: Goodwin, T. W. (ed.) Plant Pigments. Academic Press, London, pp. 61–132.Google Scholar
  13. 13.
    Hammer, Ø., Harper, D. A. T., Ryan, P. D. (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4, 9. Available at: http://palaeo-electronica.org/2001_1/past/issue1_01.htm.Google Scholar
  14. 14.
    Hansen, U., Fiedler, B., Rank, B. (2002) Variation of pigment composition and antioxidative systems along the canopy light gradient in a mixed beech/oak forest: a comparative study on deciduous tree species differing in shade tolerance. Trees 16, 354–364.CrossRefGoogle Scholar
  15. 15.
    Ishii, H., Asano, S. (2009) The role of crown architecture, leaf phenology and photosynthetic activity in promoting complementary use of light among coexisting species in temperate forests. Ecol. Res. 25, 715–722.CrossRefGoogle Scholar
  16. 16.
    Jakucs, P. (1985) Ecology of an Oak Forest in Hungary. Akadémiai Kiadó, Budapest, pp. 19–32.Google Scholar
  17. 17.
    Klughammer, C., Schreiber, U. (2008) Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the Saturation Pulse method. PAM Application Notes 1, 27–35.Google Scholar
  18. 18.
    Kull, O., Broadmeadow, M., Kruijt, B., Meir, P. (1999) Light distribution and foliage structure in an oak canopy. Trees 14, 55–64.CrossRefGoogle Scholar
  19. 19.
    Le Roux, X., Sinoquet, H., Vandame, M. (1999) Spatial distribution of leaf dry weight per area and leaf nitrogen concentration in relation to local radiation regime within an isolated tree crown. Tree Physiol. 19, 181–188.CrossRefGoogle Scholar
  20. 20.
    Lichtenthaler, H. K. (1981) Adaptation of leaves and chloroplasts to high quanta fluence rates. In: Akoyunoglou, G. (ed.) Photosynthesis VI. Balaban International Science Service, Philadelphia, pp. 273–285.Google Scholar
  21. 21.
    Lichtenthaler, H. K., Buschmann, C., Knapp, M. (2005) How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer. Photosynthetica 43, 379–393.CrossRefGoogle Scholar
  22. 22.
    Mészáros, I., Veres, S., Kanalas, P., Oláh, V., Szőllősi, E., Sárvári, É., Lévai, L., Lakatos, Gy. (2007) Leaf growth and photosynthetic performance of two co-existing oak species in contrasting growing seasons. Acta Silv. Lign. Hung. 3, 7–20.Google Scholar
  23. 23.
    Muraoka, H., Saigusa, N., Nasahara, N. K., Noda, H., Yoshino, J., Taku, M. S., Nagai, S., Murayama, S., Koizumi, H. (2010) Effects of seasonal and interannual variations in leafphotosynthesis and canopy leaf area index on gross primary production of a cool-temperate deciduous broadleaf forest in Takayama. Japan. J. Plant Res. 123, 563–576.CrossRefGoogle Scholar
  24. 24.
    Niinemets, Ü., Kull, O., Tenhunen, J. D. (1998) An analysis of light effects on foliar morphology, physiology, and light interception in temperate deciduous woody species of contrasting shade tolerance. Tree Physiol. 18, 681–696.CrossRefGoogle Scholar
  25. 25.
    Niinemets, Ü. (1998) Adjustment of foliage structure and function to a canopy light gradient in two co-existing deciduous trees. Variability in leaf inclination angles in relation to petiole morphology. Trees 12, 446–451.CrossRefGoogle Scholar
  26. 26.
    Niinemets, Ü., Valladares, F. (2006) Tolerance to shade drought and water-logging of temperate Northern Hemisphere trees and shrubs. Ecol. Monogr. 76, 521–547.CrossRefGoogle Scholar
  27. 27.
    Niinemets, Ü. (2010) A review of interception in plant stands frpm leaf to canopy in different plant functional types and in species with varying shade tolerance. Ecol. Res. 25, 693–714.CrossRefGoogle Scholar
  28. 28.
    Ort, D. R. (2001) When there is too much light. Plant Physiol. 125, 29–32.CrossRefGoogle Scholar
  29. 29.
    Rosenqvist, E., van Kooten, O. (2003) Chlorophyll fluorescence: A general description and nomenclature. In: DeEll, J. R., Toivonen, P. M. A. (eds): Practical Applications of Chlorophyll Fluorescence in Plant Biology. Kluwer Academic Publishers, Boston/Dordrecht/London, pp. 31–77.CrossRefGoogle Scholar
  30. 30.
    Sarijeva, G., Knapp, M., Lichtenthaler, H. K. (2007) Differences in photosynthetic activity, chlorophyll and carotenoid levels, and in chlorophyll fluorescence parameters in green sun and shade leaves of Ginkgo and Fagus. J. Plant Physiol. 164, 950–955.CrossRefGoogle Scholar
  31. 31.
    Schreiber, U., Bilger, W., Neubauer, C. (1994) Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze, E. D., Caldwell, M. M. (eds) Ecophysiology of photosynthesis. Springer, Berlin, pp. 49–70.Google Scholar
  32. 32.
    Thayer S. S., Björkman, O. (1990) Leaf xanthophyll content and composition in sun and shade determined by HPLC. Photosynt. Res. 23, 331–343.CrossRefGoogle Scholar
  33. 33.
    Thiele, A., Krause, G. H. (1994) Xanthophyll cycle and thermal energy dissipation in photosystem II: relationship between zeaxanthin formation, energy-dependent fluorescence quenching and photoinhibition. J. Plant Physiol. 144, 324–332.CrossRefGoogle Scholar
  34. 34.
    Turgeon, R. (1989) The sink–source transition in leaves. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 119–138.CrossRefGoogle Scholar
  35. 35.
    Valladares, F., Niinemets, Ü. (2007) The architecture of plant crowns: from design rules to light capture and performance. In: Pugnaire, F. I., Valladares, F. (eds) Handbook of Functional Plant Ecology. CRC, Boca Raton, pp. 101–149.Google Scholar
  36. 36.
    Young, A. J. (1991) The photoprotective role of carotenoids in higher plants. Physiol. Plant. 83, 702–708.CrossRefGoogle Scholar
  37. 37.
    Young, A. J., Denise, P., Ruban, A. V., Horton, P., Grank, H. A. (1997) The xanthofyll cycle and carotenoid-mediated dissipation of excess excitation energy in photosynthesis. Pure & Appl. Chem. 10, 2125–2130.CrossRefGoogle Scholar
  38. 38.
    Wellburn, A. R. (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol. 144, 307–313.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Erzsébet Szőllősi
    • 1
  • V. Oláh
    • 1
  • P. Kanalas
    • 1
  • J. Kis
    • 1
  • A. Fenyvesi
    • 2
  • Ilona Mészáros
    • 1
    Email author
  1. 1.Department of Botany, Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary
  2. 2.Section of Cyclotron ApplicationsInstitute of Nuclear ResearchDebrecenHungary

Personalised recommendations