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Acta Biologica Hungarica

, Volume 61, Issue 4, pp 457–469 | Cite as

Development of a Virus Induced Gene Silencing Vector from a Legumes Infecting Tobamovirus

  • Éva VárallyayEmail author
  • Zsuzsanna Lichner
  • Judit Sáfrány
  • Z. Havelda
  • P. Salamon
  • Gy. Bisztray
  • J. Burgyán
Article

Abstract

Medicago truncatula, the model plant of legumes, is well characterized, but there is only a little knowledge about it as a viral host. Viral vectors can be used for expressing foreign genes or for virus-induced gene silencing (VIGS), what is a fast and powerful tool to determine gene functions in plants. Viral vectors effective on Nicotiana benthamiana have been constructed from a number of viruses, however, only few of them were effective in other plants. A Tobamovirus, Sunnhemp mosaic virus (SHMV) systemi-cally infects Medicago truncatula without causing severe symptoms. To set up a viral vector for Medicago truncatula, we prepared an infectious cDNA clone of SHMV. We constructed two VIGS vectors differing in the promoter element to drive foreign gene expression. The vectors were effective both in the expression and in the silencing of a transgene Green Fluorescent Protein (GFP) and in silencing of an endogenous gene Phytoene desaturase (PDS) on N. benthamiana. Still only one of the vectors was able to successfully silence the endogenous Chlorata 42 gene in M. truncatula.

Keywords

VIGS SHMV Medicago truncatula gene silencing functional genomics 

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Notes

Acknowledgements

We wish to thank Gabor Giczey for critical reading of the manuscript. É. V. is recipients of Bolyai János Fellowship.

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© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Éva Várallyay
    • 1
    Email author
  • Zsuzsanna Lichner
    • 1
  • Judit Sáfrány
    • 1
  • Z. Havelda
    • 1
  • P. Salamon
    • 2
  • Gy. Bisztray
    • 3
  • J. Burgyán
    • 1
  1. 1.Agricultural Biotechnology CentrePlant Biology InstituteGödöllőHungary
  2. 2.Rákóczi út 14BerkeszHungary
  3. 3.Department of Viticulture, Faculty of Horticultural SciencesCorvinus University of BudapestBudapestHungary

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