Influence of Genotype and Explant Source on the In Vitro Regeneration Ability of Different Melon Varieties
Nine genotypes of melon (Cucumis melo L.) were selected for the investigation of regeneration. Most of the tested varieties showed regeneration ability on medium containing 0.5 mg 1−1 or 1 mg 1−1 BA, but following the appearance of shoot buds, only six varieties produced leafy shoots. The effect of combinations of BA with different auxins (IAA, NAA, 2,4-D) and ABA in the culture medium on shoot regeneration was tested on cotyledon expiants of ‘Hógolyó’ and ’Hale’s Best’. To establish optimal conditions for the adventitious shoot induction six types of seedling-derived expiants were prepared from seedlings of four different ages. The best results for shoot forming capacity were achieved with cotyledons followed by decapitated seedlings and hypocotyls derived from 4-day-old seedlings. Cotyledon segments of ‘Hógolyó’ and ‘Hale’s Best’ were also cultivated on media with different concentrations of IAA and BA supplemented with 0.26 mg 1−1 ABA. The highest number of well-formed plantlets was counted for ‘Hógolyó’ on the medium supplemented with 0.9 mg 1−1 BA+0.6 mg 1−1 IAA+0.26 mg 1−1 ABA. This is the first report on the in vitro regeneration of ’Hógolyó’ from decapitated seedling and hypocotyl expiants and of ‘Javitott Zentai’, ‘Muskotály’, ‘Hógolyó’, ‘Tétényi csereshéjú’ and ‘Magyar Kincs’ from cotyledon expiants.
KeywordsMelon in vitro regeneration cotyledon decapitated seedlings
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The authors would like to thank Barbara Harasztos for the critical reading and revising of the manuscript. Thanks are also due to Agnes Gyurcsa-Millei for technical assistance, and to Oliver Kiss for his help in the statistical analysis.
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