Acta Biologica Hungarica

, Volume 61, Issue 3, pp 344–355 | Cite as

The Plackett-Burman Design in Optimization of Media Components for Biomass Production of Lactobacillus Rhamnosus Oxy

  • A. Waśko
  • Monika Kordowska-Wiater
  • M. Podleśny
  • Magdalena Polak-BereckaEmail author
  • Z. Targoński
  • Agnieszka Kubik-Komar


The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production could be achieved by the combination of glucose (12.38 g/l), sodium pyruvate (3.15 g/l), meat extract (4.08 g/l), potassium phosphate (1.46 g/l), sodium acetate (3.65 g/l) and ammonium citrate (1.46 g/l).

The validation of the predicted model carried out in bioreactor conditions confirmed the usefulness of the new medium for the culture of L. rhamnosus OXY in large scale. The optimal medium makes the culture of the probiotic bacterium L. rhamnosus OXY more cost effective.


Lactobacillus biomass growth optimization response surface methodology 


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© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Waśko
    • 1
  • Monika Kordowska-Wiater
    • 1
  • M. Podleśny
    • 1
  • Magdalena Polak-Berecka
    • 1
    Email author
  • Z. Targoński
    • 1
  • Agnieszka Kubik-Komar
    • 2
  1. 1.Department of Biotechnology, Human Nutrition and Food CommoditiesUniversity of Life SciencesLublinPoland
  2. 2.Department of Applied Mathematics and Computer ScienceUniversity of Life SciencesLublinPoland

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