Investigation of Potential Stress Parameters in Rainbow Trout (Oncorhynchus Mykiss)
Abstract
The transportation of rainbow trout in the presence of the anesthetic clove oil was investigated. Before the transportation tests, an acute experiment was conducted to verify that removal of the fish from the water for one minute does not significantly increase the glucose or Cortisol concentration of the blood plasma. In the main experiment two different transportation conditions were compared: transport in water only and in water with anesthetic. During transportation without addition of clove oil, blood plasma glucose and Cortisol concentrations changed significantly. The concentration of glucose increased from 4.92 mmol/L prior to transportation to 6.16 mmol/L and values similar to the initial ones (4.95 mmol/L) were observed 5 hours after transportation. Concentration of the stress hormone Cortisol increased from the initial 37.2 ng/mL to 89.2 ng/mL and returned to a value of 36.1 ng/mL 3 hours post transportation. Respective values of glucose concentration have not changed significantly during transportation in the presence of clove oil (4.3; 4.4; 4.4 mmol/L), whereas those of Cortisol showed a slight decrease with the passing of time (28.1; 26.7; 20.18 ng/mL). Results show that transportation stress can significantly be reduced by the use of anesthetics.
Keywords
Fish transport stress rainbow trout clove oilPreview
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References
- 1.Anderson, W. G., McKinley, R. S., Colavecchia, M. (1997) The use of clove oil as an anaesthetic for rainbow trout and its effects on swimming performance. N. Am. J. Fish Manage. 17, 301–307.CrossRefGoogle Scholar
- 2.Briozzo, J. L., Chirife, J., Herzage, L., D’Aquino, M. (1989) Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. J. App. Bacteriol. 66, 69–75.CrossRefGoogle Scholar
- 3.Bullerman, L. B., Lieu, F. Y., Seier, S. A. (1977) Inhibition of growth and aflatoxin production by cinnamon and clove oil, cinnamic aldehyde and eugenol. J. Food. Sci. 42, 1107–1116.CrossRefGoogle Scholar
- 4.Cooke, S. J., Suski, C. D., Ostrand, K. G., Tufts, B. L., Wahl, D. H. (2004) Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting large-mouth bass (Micropterus salmoides). Aquaculture 239, 509–529.CrossRefGoogle Scholar
- 5.Demers, N. E., Bayne, C. J. (1997) The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Dev. Comp. Immun. 21, 363–373.CrossRefGoogle Scholar
- 6.Dziaman, R., Klyszejko, B., Hajek, G. (2005) The effects of MS-222 on the cardiac and respiratory function and behaviour of common carp, Cyprinus carpio L., during general anaesthesia. Acta Ichthyol. Piscat. 35, 125–131.CrossRefGoogle Scholar
- 7.Hajek, G. J., Klyszejko, B. (2004) The effects of Propiscin (etomidate) on the behaviour, heart rate, and ventilation of common carp, Cyprinus carpio L. Acta Ichthyol. Piscat. 34, 129–143.CrossRefGoogle Scholar
- 8.Inoue, L., Afonso, L. B., Iwama, G. K., Moraes, G. (2005) Effects of clove oil on the stress response of matrinxã (Brycon cephalus) subjected to transport. Acta Amaz. 35, 289–295.CrossRefGoogle Scholar
- 9.Isaacs, G. (1983) Permanent local anaesthesia and anhydrosis after clove oil spillage. Lancet. 1, 882–883.CrossRefGoogle Scholar
- 10.Iversen, M., Finstad, B., McKinley, R. S., Eliassen, R. A. (2003) The efficacy of metomidate, clove oil, Aqui-STM and Benzoak® as anaesthetics in Atlantic salmon (Salmo salar L.) smolts, and their potential stress-reducing capacity. Aquaculture 221, 549–566.CrossRefGoogle Scholar
- 11.Iversen, M., Eliassen, R. A., Finstad, B. (2009) Potential benefit of clove oil sedation on animal welfare during salmon smolt, Salmo salar L. transport and transfer to sea. Aquac. Res. 40, 233–241.CrossRefGoogle Scholar
- 12.Karapinar, M. (1990) Inhibitory effects of anethole and eugenol on the growth and toxic production of Aspergilus parasiticus. Int. J. Food. Microbiol. 10, 193–200.CrossRefGoogle Scholar
- 13.Karapinar, M., Aktug, S. E. (1987) Inhibition of foodborne pathogens by thymol, eugenol, menthol anethole. Int. J. Food. Microbiol. 4, 161–166.CrossRefGoogle Scholar
- 14.Kazun, K., Siwicki, A. K. (2001) Propiscin: a safe new anaesthetic for fish. Arch. Polish Fish. 9, 183–190.Google Scholar
- 15.Keene, J. L., Noakes, D. L. G., Moccia, R. D., Soto, C. G. (1998) The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquac. Res. 29, 89–101.CrossRefGoogle Scholar
- 16.Kildea, M. A., Allan, G. L., Kearney, R. E. (2004) Accumulation and clearance of the anaesthetics clove oil and AQUI-S (TM) from the edible tissue of silver perch (Bidyanus bidyanus). Aquaculture 232, 265–277.CrossRefGoogle Scholar
- 17.Kremer, E. R. (1985) Antioxidants in clove. J. Am. Oil. Chem. Soc. 62, 111–113.CrossRefGoogle Scholar
- 18.Lyytikainen, T., Pylkko, P., Ritola, O., Lindstrom-Seppa, P. (2002) The effect of acute stress and temperature on plasma Cortisol and ion concentrations and growth of Lake Inari Arctic charr, Salvelinus alpinus. Environ. Biol. Fish. 64, 195–202.CrossRefGoogle Scholar
- 19.Matuk, K. (1987) Novel possibilities in anasthesation offish. Halâszat 80, 11–13. (In Hungarian).Google Scholar
- 20.McFarland, W. N. (1960) The use of anesthetics for the handling and the transport of fishes. Calif. Fish. Game 46, 407–431.Google Scholar
- 21.Moleyar, V., Narasimham, P. (1992) Antibacterial activity of essential oil components. Int. J. Food. Microbiol. 16, 337–342.CrossRefGoogle Scholar
- 22.Munday, P. L., Wilson, S. K. (1997) Comparative efficacy of clove oil and other chemicals in anaesthetization of Pomacentrus amboinensis, a coral reef fish. J. Fish. Biol. 51, 931–938.Google Scholar
- 23.Nagababu, E., Lakshmaiah, N. (1992) Inhibitory effects of eugenol on non-enzymatic lipid peroxidation in rat liver mitochondria. Biochem. Pharmacol. 43, 2393–2400.CrossRefGoogle Scholar
- 24.Peake, S. (1998) Sodium bicarbonate and clove oil as potential anaesthetics for nonsalmonid fishes. N. Am. J. Fish. Manage 18, 919–924.CrossRefGoogle Scholar
- 25.Pulla Reddy, A. C. H., Lokesh, B. R. (1992) Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes. Mol. Cell. Biochem. 111, 117–124.CrossRefGoogle Scholar
- 26.Rajakumar, D. V., Rao, M. N. A. (1993) Dehydrozingerone and isoeugenol as inhibitors of lipid peroxidation and as free radical scavengers. Biochem. Pharmacol. 46, 2067–2072.CrossRefGoogle Scholar
- 27.Ross, L. G., Ross, B. (1999) Anaesthetic and Sedative Techniques for Aquatic Animals. 2nd ed. Blackwell Science Ltd., Oxford. 159.Google Scholar
- 28.Soto, C. G., Burhanuddin, S. (1995) Clove oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture 136, 149–152.CrossRefGoogle Scholar
- 29.Summerfeit, R. C., Smith, L. S. (1990) Anesthesia, surgery, and related techniques. In: Schreck, C. B., Moyle, P. B. (eds) Methods for fish biology. American Fisheries Society, Bethesda, Maryland, pp. 213–272.Google Scholar
- 30.Taylor, P. W., Roberts, S. D. (1999) Clove oil: An alternative anaesthetic for aquaculture. N. Am. J. Aquaculture 61, 150–155.CrossRefGoogle Scholar
- 31.Tytler, P., Hawkins, A. D. (1981) Vivisection, anaesthetics and minor surgery. In: Hawkins, A. D. (ed.) Aquarium systems. Academic Press, New York, pp. 247–278.Google Scholar
- 32.Wagner, G. N., Singer, T. D., McKinley, S. R. (2003) The ability of clove oil and MS-222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum). Aquac. Res. 34, 1139–1146.CrossRefGoogle Scholar
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