This study was performed to investigate the effects of L-thyroxine treatment on plasma vasopressin (AVP) levels in rats with hypothyroidism induced by propylthiouracil (PTU). Animals were separated into three groups each having 6 rats: control, PTU, PTU+L-thyroxine groups. Then, the groups were further divided into 3 sub-groups including 6 rats (a; basal, b; hypertonic stimulated and c; hypovolemic stimulated). At the end of the experiments all rats were decapitated in order to obtain plasma samples for analysis in terms of Hct, osmolality, TT3, TT4 and vasopressin. Haematocrit (Hct) levels were the highest in hypovolemic stimulated sub-group (P < 0.001). Osmolality levels were higher in hypertonic stimulated sub-groups (P < 0.001). Total T3 and T4 values were the lowest in the PTU group and the highest in the L-thyroxine treated group (P < 0.001). Plasma AVP levels were reduced by hypothyroidism. However, L-thyroxine treatment after the hypothyroidism prevented this reduction (P < 0.001). Vasopressin responses to basal, hypovolemic and hypertonic stimulations were the lowest in the PTU group (P < 0.001). The results of the present study show that basal and stimulated plasma vasopressin levels are reduced in PTU-induced hypothyroidism. However, L-thyroxine treatment following hypothyroidism prevents this reduction.
Ali, M., Guillon, G., Clos, J. (1988) Comparative study of collecting tabules and vasopressin binding capacity in renal medulla of developing hypothyroid rat. J. Dev. Physiol. 15, 297–308.
Ali, M., Guillon, G., Balestre, M. N., Clos, J. (1987) Effects of thyroid deficiency on the vasopressin receptors in the kidney of developing and adult rats. A comparative study of hormonal binding and adenylate cyclase activation. Horm. Metab. Res. 19, 115–121.
Ali, M., Guillon, G., Cantau, B., Balestre, M. N., Chicot, D., Clos, J. (1987) A comparative study of plasma vasopressin levels and V1 and V2 vasopressin receptor properties in congenital hypothyroid rat under thyroxine or vasopressin therapy. Horm. Metab. Res. 19, 624–628.
Carter, D. A., Pardy, K., Murphy, D. (1993) Regulation of vasopressin gene expression: Changes in the level but not the size of vasopressin mRNA following endocrine manipulations. Cell. Mol. Neurobiol. 13, 87–95.
Chen, Y. C., Cadnapaphornchai, M. A., Yang, J., Summer, S. N., Falk, S., Li, C. (2005) Non-osmotic release of vasopressin and renal aquaporin in impaired urinary dilution in hypothyroidism. Am. J. Physiol. Renal Physiol. 289, F672–678.
Ciosek, J., Drobnik, J. (2004) Vasopressin and oxytocin release and the thyroid function. J. Physiol. Pharmacol. 55, 423–441.
Dakine, N., Oliver, C., Grino, M. (2000) Effects of experimental hypothyroidism on the development of the hypothalamic-pituitary-adrenal axis in the rat. Life Sci. 67, 2827–2844.
Forsling, M. L., Paeysner, K. (1988) Pituitary and plasma vasopressin concentrations and fluid balance throughout the estrus cycle of the rat. J. Endocrinol. 117, 397–402.
Forsling, M. L., Kelestimur, H., Windle, R. (1991) The influence of reproductive status on vasopressin release in the rat. J. Physiol. 414, 45.
Hayashi, M., Arima, H., Goto, M., Banno, R., Watanabe, M., Sato, I. (2006) Vasopressin gene transcription increases in response to decreases in plasma volume, but not to increases in plasma osmolality, in chronically dehydrated rats. Am. J. Physiol. Endocrinol. Metab. 290, E213–217.
Iwasaki, Y., Osio, Y., Yamauchi, K. (1990) Osmoregulation of plasma vasopressin in mix edema. J. Clin. Endocrinol. Metab. 70, 534–539.
Kasting, N. W. (1988) Simultaneous and independent release of vasopressin and oxytocin in the rat. Can. J. Physiol. Pharmacol. 66, 22–26.
Koechlin, N., Elalouf, J. M., Kaissling, B., Roinel, R., Rouffignac, C. (1989) A structural study of the rat proximal and distal nephron: Effect of peptide and thyroid hormones. Am. J. Physiol. 256, F814–822.
Kondo, N., Arima, H., Banno, R., Kuwahara, S., Sato, I., Oiso, Y. (2004) Osmoregulation of vasopressin release and gene transcription under acute and chronic hypovolemia in rats. Am. J. Physiol. Endocrinol. Metab. 286, 437–446.
Kossler, A., Hagmuller, K., Winkler, R. A. (1989) Structural study of the rat proximal and distal nephron: effect of peptide and thyroid hormones. Am. J. Physiol. 256, 814–822.
Marcisz, C., Jonderko, G., Kucharz, E. J. (2001) Changes of plasma arginine vasopressin level in patients with hyperthyroidism during treatment. Med. Sci. Mon. 7, 409–414.
Mccormick, S. D., Bradshaw, D. (2006) Hormonal control of salt and water balance in vertebrates. Gen. Comp. Endocrinol. 147, 3–8.
Melissa, A. C., Yang-Wook, K., Andrey, K. G. (2003) Urinary concentrating defect in hypothyroid rats: Role of sodium, potassium, 2-chloride co-transporter, and aquaporins. J. Am. Soc. Nephrol. 14, 566–574.
Mogulkoc, R., Baltaci, A.K., Yilmaz, B., Kelesfimur, H. (1999) Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the propylthiouracil-induced hypothyroid rats. Neuroendocrinal. Lett. 20, 311–314.
Mogulkoc, R., Dogru, O., Baltaci, A. K., Yilmaz, B., Kelestimur, H. (2000) Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the thyroidectomy-induced hypothyroid rats. Neuroendocrinal. Lett. 21, 35–38.
Park, C. W., Shin, Y. S., Ahn, S. J., Kim, S. J., Choi, E. J., Chang, Y. S. (2001) Thyroxine treatment induces upregulation of renin-angiotensin-aldosterone system due to decreasing effective plasma volume in patients with primary myxoedema. Nephrol. Dial. Transplant. 16, 1799–1806.
Rolandi, E., Santaniello, B., Bagnasco, M., Cataldi, A., Garibaldi, C., Franceschini, R., Barreca, T. (1992) Thyroid hormones and atrial natriuretic hormone secretion: study in hyper- and hypothyroid patients. Acta Endocrinol. 127, 23–26.
Rosato, R. R., Gimenez, M. S., Jahn, G. A. (1992) Effects of chronic thyroid hormone administration on pregnancy, lactogenesis and lactation in the rat. Acta Endocrinol. 127, 547–554.
Sahun, M., Ramon, J. M., Navarro, M. A. (2001) Water metabolism disturbances of different stages of primary thyroid failure. J. Endocrinol. 168, 435–445.
Salomez-Granier, F., Lefebvre, J., Racadot, A., Dewailly, D., Linquette, M. (1983) Antidiuretic hormone levels (arginine-vasopressin) in cases of peripheral hypothyroidism, 26 cases. Presse Médical. 12, 1001–1004.
Schmitt, R., Klussmann, E., Kahl, T., Ellison, D. H., Bachmann, S. (2003) Renal expression of sodium transporters and aquaporin-2 in hypothyroid rats. Am. J. Physiol. Renal. Physiol. 284, F1097–1104.
Shinohara, R., Mano, T., Nagasaka, A., Hayashi, R., Uchimura, K., Nakano, I. (2000) Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status. J. Endocrinol. 164, 97–102.
Stempniak, B., Marczynski, A., Dobrowski, R. (2002) Disorders of thyroid function and hormone release from the rat pituitary. Pol. J. Endocrinol. 53, 13–22.
Vargas, F., Baz, M. J., Luna, J. D., Andrade, J., Jodar, E., Haro, J. M. (1991) Urinary excretion of digoxin-like immunoreactive factor and arginine-vasopressin in hyper- and hypothyroid rats. Clin. Sci. 81, 471–476.
Wang, W., Li, C., Summer, S. N., Falk, S., Schrier, R. W. (2007) Polyuria of thyrotoxicosis: down-regulation of aquaporin water channels and increased solute excretion. Kidney Int. 72, 1088–1094.
Yamaguchi, K., Yamada, T. (2006) Involvement of anteroventral third ventricular AMPA/kainate receptors in both hyperosmotic and hypovolemic AVP secretion in conscious rats. Brain Res. Bull. 71, 183–192.
Zimmerman, R. S., Ryan, J., Edwards, B. S., Klee, G., Zimmerman, D., Scott, N. (1988) Cardiorenal endocrine dynamics during volume expansion in hypothyroid dogs. Am. J. Physiol. 255, 61–66.
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Aydin, L., Mogulkoc, R. & Baltaci, A.K. Influences of Hypertonic and Hypovolemic Treatments on Vasopressin Response in Propylthiouracil (PTU) Induced Hypothyroid Ratand Effect on Supplementation with L-Thyroxine. BIOLOGIA FUTURA 61, 1–9 (2010). https://doi.org/10.1556/ABiol.61.2010.1.1
- thyroxine treatment