Advertisement

Acta Biologica Hungarica

, Volume 61, Issue 1, pp 1–9 | Cite as

Influences of Hypertonic and Hypovolemic Treatments on Vasopressin Response in Propylthiouracil (PTU) Induced Hypothyroid Ratand Effect on Supplementation with L-Thyroxine

  • Leyla Aydin
  • R. MogulkocEmail author
  • A. K. Baltaci
Article

Abstract

This study was performed to investigate the effects of L-thyroxine treatment on plasma vasopressin (AVP) levels in rats with hypothyroidism induced by propylthiouracil (PTU). Animals were separated into three groups each having 6 rats: control, PTU, PTU+L-thyroxine groups. Then, the groups were further divided into 3 sub-groups including 6 rats (a; basal, b; hypertonic stimulated and c; hypovolemic stimulated). At the end of the experiments all rats were decapitated in order to obtain plasma samples for analysis in terms of Hct, osmolality, TT3, TT4 and vasopressin. Haematocrit (Hct) levels were the highest in hypovolemic stimulated sub-group (P < 0.001). Osmolality levels were higher in hypertonic stimulated sub-groups (P < 0.001). Total T3 and T4 values were the lowest in the PTU group and the highest in the L-thyroxine treated group (P < 0.001). Plasma AVP levels were reduced by hypothyroidism. However, L-thyroxine treatment after the hypothyroidism prevented this reduction (P < 0.001). Vasopressin responses to basal, hypovolemic and hypertonic stimulations were the lowest in the PTU group (P < 0.001). The results of the present study show that basal and stimulated plasma vasopressin levels are reduced in PTU-induced hypothyroidism. However, L-thyroxine treatment following hypothyroidism prevents this reduction.

Keywords

Hypothyroidism thyroxine treatment AVP osmolality rat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ali, M., Guillon, G., Clos, J. (1988) Comparative study of collecting tabules and vasopressin binding capacity in renal medulla of developing hypothyroid rat. J. Dev. Physiol. 15, 297–308.Google Scholar
  2. 2.
    Ali, M., Guillon, G., Balestre, M. N., Clos, J. (1987) Effects of thyroid deficiency on the vasopressin receptors in the kidney of developing and adult rats. A comparative study of hormonal binding and adenylate cyclase activation. Horm. Metab. Res. 19, 115–121.CrossRefGoogle Scholar
  3. 3.
    Ali, M., Guillon, G., Cantau, B., Balestre, M. N., Chicot, D., Clos, J. (1987) A comparative study of plasma vasopressin levels and V1 and V2 vasopressin receptor properties in congenital hypothyroid rat under thyroxine or vasopressin therapy. Horm. Metab. Res. 19, 624–628.CrossRefGoogle Scholar
  4. 4.
    Carter, D. A., Pardy, K., Murphy, D. (1993) Regulation of vasopressin gene expression: Changes in the level but not the size of vasopressin mRNA following endocrine manipulations. Cell. Mol. Neurobiol. 13, 87–95.CrossRefGoogle Scholar
  5. 5.
    Chen, Y. C., Cadnapaphornchai, M. A., Yang, J., Summer, S. N., Falk, S., Li, C. (2005) Non-osmotic release of vasopressin and renal aquaporin in impaired urinary dilution in hypothyroidism. Am. J. Physiol. Renal Physiol. 289, F672–678.CrossRefGoogle Scholar
  6. 6.
    Ciosek, J., Drobnik, J. (2004) Vasopressin and oxytocin release and the thyroid function. J. Physiol. Pharmacol. 55, 423–441.Google Scholar
  7. 7.
    Dakine, N., Oliver, C., Grino, M. (2000) Effects of experimental hypothyroidism on the development of the hypothalamic-pituitary-adrenal axis in the rat. Life Sci. 67, 2827–2844.CrossRefGoogle Scholar
  8. 8.
    Forsling, M. L., Paeysner, K. (1988) Pituitary and plasma vasopressin concentrations and fluid balance throughout the estrus cycle of the rat. J. Endocrinol. 117, 397–402.CrossRefGoogle Scholar
  9. 9.
    Forsling, M. L., Kelestimur, H., Windle, R. (1991) The influence of reproductive status on vasopressin release in the rat. J. Physiol. 414, 45.Google Scholar
  10. 10.
    Hayashi, M., Arima, H., Goto, M., Banno, R., Watanabe, M., Sato, I. (2006) Vasopressin gene transcription increases in response to decreases in plasma volume, but not to increases in plasma osmolality, in chronically dehydrated rats. Am. J. Physiol. Endocrinol. Metab. 290, E213–217.CrossRefGoogle Scholar
  11. 11.
    Iwasaki, Y., Osio, Y., Yamauchi, K. (1990) Osmoregulation of plasma vasopressin in mix edema. J. Clin. Endocrinol. Metab. 70, 534–539.CrossRefGoogle Scholar
  12. 12.
    Kasting, N. W. (1988) Simultaneous and independent release of vasopressin and oxytocin in the rat. Can. J. Physiol. Pharmacol. 66, 22–26.CrossRefGoogle Scholar
  13. 13.
    Koechlin, N., Elalouf, J. M., Kaissling, B., Roinel, R., Rouffignac, C. (1989) A structural study of the rat proximal and distal nephron: Effect of peptide and thyroid hormones. Am. J. Physiol. 256, F814–822.Google Scholar
  14. 14.
    Kondo, N., Arima, H., Banno, R., Kuwahara, S., Sato, I., Oiso, Y. (2004) Osmoregulation of vasopressin release and gene transcription under acute and chronic hypovolemia in rats. Am. J. Physiol. Endocrinol. Metab. 286, 437–446.CrossRefGoogle Scholar
  15. 15.
    Kossler, A., Hagmuller, K., Winkler, R. A. (1989) Structural study of the rat proximal and distal nephron: effect of peptide and thyroid hormones. Am. J. Physiol. 256, 814–822.Google Scholar
  16. 16.
    Marcisz, C., Jonderko, G., Kucharz, E. J. (2001) Changes of plasma arginine vasopressin level in patients with hyperthyroidism during treatment. Med. Sci. Mon. 7, 409–414.Google Scholar
  17. 17.
    Mccormick, S. D., Bradshaw, D. (2006) Hormonal control of salt and water balance in vertebrates. Gen. Comp. Endocrinol. 147, 3–8.CrossRefGoogle Scholar
  18. 18.
    Melissa, A. C., Yang-Wook, K., Andrey, K. G. (2003) Urinary concentrating defect in hypothyroid rats: Role of sodium, potassium, 2-chloride co-transporter, and aquaporins. J. Am. Soc. Nephrol. 14, 566–574.CrossRefGoogle Scholar
  19. 19.
    Mogulkoc, R., Baltaci, A.K., Yilmaz, B., Kelesfimur, H. (1999) Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the propylthiouracil-induced hypothyroid rats. Neuroendocrinal. Lett. 20, 311–314.Google Scholar
  20. 20.
    Mogulkoc, R., Dogru, O., Baltaci, A. K., Yilmaz, B., Kelestimur, H. (2000) Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the thyroidectomy-induced hypothyroid rats. Neuroendocrinal. Lett. 21, 35–38.Google Scholar
  21. 21.
    Park, C. W., Shin, Y. S., Ahn, S. J., Kim, S. J., Choi, E. J., Chang, Y. S. (2001) Thyroxine treatment induces upregulation of renin-angiotensin-aldosterone system due to decreasing effective plasma volume in patients with primary myxoedema. Nephrol. Dial. Transplant. 16, 1799–1806.CrossRefGoogle Scholar
  22. 22.
    Rolandi, E., Santaniello, B., Bagnasco, M., Cataldi, A., Garibaldi, C., Franceschini, R., Barreca, T. (1992) Thyroid hormones and atrial natriuretic hormone secretion: study in hyper- and hypothyroid patients. Acta Endocrinol. 127, 23–26.CrossRefGoogle Scholar
  23. 23.
    Rosato, R. R., Gimenez, M. S., Jahn, G. A. (1992) Effects of chronic thyroid hormone administration on pregnancy, lactogenesis and lactation in the rat. Acta Endocrinol. 127, 547–554.CrossRefGoogle Scholar
  24. 24.
    Sahun, M., Ramon, J. M., Navarro, M. A. (2001) Water metabolism disturbances of different stages of primary thyroid failure. J. Endocrinol. 168, 435–445.CrossRefGoogle Scholar
  25. 25.
    Salomez-Granier, F., Lefebvre, J., Racadot, A., Dewailly, D., Linquette, M. (1983) Antidiuretic hormone levels (arginine-vasopressin) in cases of peripheral hypothyroidism, 26 cases. Presse Médical. 12, 1001–1004.Google Scholar
  26. 26.
    Schmitt, R., Klussmann, E., Kahl, T., Ellison, D. H., Bachmann, S. (2003) Renal expression of sodium transporters and aquaporin-2 in hypothyroid rats. Am. J. Physiol. Renal. Physiol. 284, F1097–1104.CrossRefGoogle Scholar
  27. 27.
    Shinohara, R., Mano, T., Nagasaka, A., Hayashi, R., Uchimura, K., Nakano, I. (2000) Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status. J. Endocrinol. 164, 97–102.CrossRefGoogle Scholar
  28. 28.
    Stempniak, B., Marczynski, A., Dobrowski, R. (2002) Disorders of thyroid function and hormone release from the rat pituitary. Pol. J. Endocrinol. 53, 13–22.Google Scholar
  29. 29.
    Vargas, F., Baz, M. J., Luna, J. D., Andrade, J., Jodar, E., Haro, J. M. (1991) Urinary excretion of digoxin-like immunoreactive factor and arginine-vasopressin in hyper- and hypothyroid rats. Clin. Sci. 81, 471–476.CrossRefGoogle Scholar
  30. 30.
    Wang, W., Li, C., Summer, S. N., Falk, S., Schrier, R. W. (2007) Polyuria of thyrotoxicosis: down-regulation of aquaporin water channels and increased solute excretion. Kidney Int. 72, 1088–1094.CrossRefGoogle Scholar
  31. 31.
    Yamaguchi, K., Yamada, T. (2006) Involvement of anteroventral third ventricular AMPA/kainate receptors in both hyperosmotic and hypovolemic AVP secretion in conscious rats. Brain Res. Bull. 71, 183–192.CrossRefGoogle Scholar
  32. 32.
    Zimmerman, R. S., Ryan, J., Edwards, B. S., Klee, G., Zimmerman, D., Scott, N. (1988) Cardiorenal endocrine dynamics during volume expansion in hypothyroid dogs. Am. J. Physiol. 255, 61–66.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Physiology, Meram Medical SchoolSelcuk UniversityKonyaTurkey

Personalised recommendations