Spatial Pattern Analysis of Nitrergic Neurons in the Myenteric Plexus of the Duodenum of Different Mammalian Species

Abstract

Nitrergic myenteric neurons are especially susceptible to the development of neuropathy in functional gastrointestinal disorders. Investigations of the similarities and dissimilarities in the organization of nitrergic neurons in the various mammalian species are therefore important in an effort to determine the extent to which the results obtained in different animal models can be generalized. In the present work, the density and the spatial organization of the nitrergic neurons in the myenteric plexus of the duodenum were investigated in 7 mammalian species. After nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, the Plexus Pattern Analysis software (PPAs) was applied to count the nuclei of nitrergic neurons, calculate the proportions of the areas covered by the plexus and perform randomization analysis. All 7 species exhibited a large population of nitrergic myenteric neurons, with densities in the range 12–56 cells/mm2. The distribution patterns of these neurons differed markedly in the different species, however, the rat was the only species in which the nitrergic neurons appeared to be randomly distributed. The PPAs in conjunction with NADPH-d histochemistry proved to be a simple and fast tool with which to reveal similarities and dissimilarities in the spatial arrangement of the nitrergic neurons in the different species.

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Acknowledgement

Supported by a grant from the National Scientific Research Programs (OTKA) No. F46201 to M.B.

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Correspondence to Mária Bagyánszki.

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Bódi, N., Battonyai, I., Talapka, P. et al. Spatial Pattern Analysis of Nitrergic Neurons in the Myenteric Plexus of the Duodenum of Different Mammalian Species. BIOLOGIA FUTURA 60, 347–358 (2009). https://doi.org/10.1556/ABiol.60.2009.4.2

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Keywords

  • Nitrergic myenteric neurons
  • NADPH-diaphorase histochemistry
  • duodenum
  • Plexus Pattern Analysis software
  • comparative study