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Acta Biologica Hungarica

, Volume 60, Issue 4, pp 347–358 | Cite as

Spatial Pattern Analysis of Nitrergic Neurons in the Myenteric Plexus of the Duodenum of Different Mammalian Species

  • Nikolett Bódi
  • Izabella Battonyai
  • Petra Talapka
  • Éva Fekete
  • Mária BagyánszkiEmail author
Article

Abstract

Nitrergic myenteric neurons are especially susceptible to the development of neuropathy in functional gastrointestinal disorders. Investigations of the similarities and dissimilarities in the organization of nitrergic neurons in the various mammalian species are therefore important in an effort to determine the extent to which the results obtained in different animal models can be generalized. In the present work, the density and the spatial organization of the nitrergic neurons in the myenteric plexus of the duodenum were investigated in 7 mammalian species. After nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, the Plexus Pattern Analysis software (PPAs) was applied to count the nuclei of nitrergic neurons, calculate the proportions of the areas covered by the plexus and perform randomization analysis. All 7 species exhibited a large population of nitrergic myenteric neurons, with densities in the range 12–56 cells/mm2. The distribution patterns of these neurons differed markedly in the different species, however, the rat was the only species in which the nitrergic neurons appeared to be randomly distributed. The PPAs in conjunction with NADPH-d histochemistry proved to be a simple and fast tool with which to reveal similarities and dissimilarities in the spatial arrangement of the nitrergic neurons in the different species.

Keywords

Nitrergic myenteric neurons NADPH-diaphorase histochemistry duodenum Plexus Pattern Analysis software comparative study 

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Notes

Acknowledgement

Supported by a grant from the National Scientific Research Programs (OTKA) No. F46201 to M.B.

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© Akadémiai Kiadó, Budapest 2009

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Nikolett Bódi
    • 1
  • Izabella Battonyai
    • 1
  • Petra Talapka
    • 1
  • Éva Fekete
    • 1
  • Mária Bagyánszki
    • 1
    Email author
  1. 1.Department of Physiology, Anatomy and NeuroscienceUniversity of SzegedSzegedHungary

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