Acta Biologica Hungarica

, Volume 60, Issue 3, pp 321–328 | Cite as

Optimization of Coprogen Production in Neurospora crassa

  • Viktória Tóth
  • K. Antal
  • Gyöngyi Gyémánt
  • M. Miskei
  • I. Pócsi
  • T. EmriEmail author


Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N5-monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures.


Siderophore coprogen Neurospora crassa response surface methodology L-ornithine-N5-monooxygenase 


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This project was supported financially by the Hungarian National Office for Research and Technology (grant reference number OMFB 01501/2006), the GENOMNANOTECH-DEBRET (RET-06/2004) and by the SOLE-MEAT Ltd., Szolnok, Hungary.


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© Akadémiai Kiadó, Budapest 2009

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Viktória Tóth
    • 1
  • K. Antal
    • 2
  • Gyöngyi Gyémánt
    • 3
  • M. Miskei
    • 4
  • I. Pócsi
    • 1
  • T. Emri
    • 1
    Email author
  1. 1.Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary
  2. 2.Department of Zoology, Faculty of SciencesEszterházy Károly CollegeEgerHungary
  3. 3.Department of Biochemistry, Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary
  4. 4.Department of Horticultural Sciences and Plant Biotechnology, Faculty of AgricultureUniversity of DebrecenDebrecenHungary

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