Acta Biologica Hungarica

, Volume 60, Issue 2, pp 195–210 | Cite as

UV-B Response of Greening Barley Seedlings

  • Ivanka FedinaEmail author
  • Maya Velitchkova
  • Katya Georgieva
  • Dimitrina Nedeva
  • H. Çakirlar


The relationship between the greening stage of barley seedlings and their response to UV-B irradiation was studied. Etiolated barley seedlings (Hordeum vulgare L., cv. Alfa) greened 12, 24 and 48 h were exposed to UV-B irradiation (312 nm) for 5 h. As a result of UV-B treatment the rate of CO2 fixation and chlorophyll contents decreased but flavonoids, UV-B-induced compounds and carotenoids increased. The inhibition of photosynthesis in green plants was lower in comparison to greening ones. The 12 h greening plants were more sensitive to UV-B treatment than the plants greening 24 h and particularly 48 h, estimated by the quantum efficiency of PSII photochemistry and the oxygen production rate. The levels of flavonoids and UV-B induced compounds enhanced with increasing the greening time. Activity of antioxidant enzymes catalase, peroxidase and superoxide dismutase increased during the seedlings greening and as a result of UV-B irradiation, but the pattern of isoforms remained similar to those found in the controls. UV-B preferentially induced Cu,Zn-superoxide dismutase. Increase of UVB induced synthesis of antioxidant enzymes is in line with their important role in the plant response to UV-B stress. Data presented show that the response of barley seedlings to UV-B irradiation is related to the development stage of photosynthetic apparatus.


Antioxidant enzymes chlorophyll fluorescence flavonoids oxygen evolution UV-B radiation 


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© Akadémiai Kiadó, Budapest 2009

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Authors and Affiliations

  • Ivanka Fedina
    • 1
    Email author
  • Maya Velitchkova
    • 2
  • Katya Georgieva
    • 1
  • Dimitrina Nedeva
    • 1
  • H. Çakirlar
    • 3
  1. 1.Department Plant Stress Molecular Biology, Academic Metodi Popov Institute of Plant PhysiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of Photoexcitable MembranesInstitute of Biophysics, Bulgarian Academy of SciencesSofiaBulgaria
  3. 3.Department of BiologyHacettepe UniversityBeytepe, AnkaraTurkey

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