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Acta Biologica Hungarica

, Volume 60, Issue 2, pp 195–210 | Cite as

UV-B Response of Greening Barley Seedlings

  • Ivanka FedinaEmail author
  • Maya Velitchkova
  • Katya Georgieva
  • Dimitrina Nedeva
  • H. Çakirlar
Article

Abstract

The relationship between the greening stage of barley seedlings and their response to UV-B irradiation was studied. Etiolated barley seedlings (Hordeum vulgare L., cv. Alfa) greened 12, 24 and 48 h were exposed to UV-B irradiation (312 nm) for 5 h. As a result of UV-B treatment the rate of CO2 fixation and chlorophyll contents decreased but flavonoids, UV-B-induced compounds and carotenoids increased. The inhibition of photosynthesis in green plants was lower in comparison to greening ones. The 12 h greening plants were more sensitive to UV-B treatment than the plants greening 24 h and particularly 48 h, estimated by the quantum efficiency of PSII photochemistry and the oxygen production rate. The levels of flavonoids and UV-B induced compounds enhanced with increasing the greening time. Activity of antioxidant enzymes catalase, peroxidase and superoxide dismutase increased during the seedlings greening and as a result of UV-B irradiation, but the pattern of isoforms remained similar to those found in the controls. UV-B preferentially induced Cu,Zn-superoxide dismutase. Increase of UVB induced synthesis of antioxidant enzymes is in line with their important role in the plant response to UV-B stress. Data presented show that the response of barley seedlings to UV-B irradiation is related to the development stage of photosynthetic apparatus.

Keywords

Antioxidant enzymes chlorophyll fluorescence flavonoids oxygen evolution UV-B radiation 

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References

  1. 1.
    Adamse, P., Britz, S. J. (1992) Amelioration of UV-B damage under high irradiance. I. Role of photosynthesis. Photochem. Photobiol. 56, 645–650.Google Scholar
  2. 2.
    Allen, D. J., McKee, I. F., Farage, P. K., Baker, N. R. (1997) Analysis of the limitation to CO2 assimilation on the exposure of leaves of two Brassica napus cultivars to UV-B. Plant Cell Environ. 20, 633–640.Google Scholar
  3. 3.
    Bates, L. S., Waldren, R. O., Teare, I. D. (1973) Rapid determination of free proline for water-stress studies. Plant Soi. 39, 205–207.Google Scholar
  4. 4.
    Caldwell, M. M. (1971) Solar UV irradiation and the growth and development of higher plants. In: Giese, A. C. (ed.) Photophysiology. tAcademic Press, New York, pp. 131–177.Google Scholar
  5. 5.
    Caldwell, M. M., Björn, L. O., Bornman, J. F., Flint, S. D., Kulandaivelu, G., Teramura, A. H., Tevini, M. (1998) Effects of increased solar ultraviolet radiation on terrestrial ecosystems. J. Photochem. Photobiol. B Biol. 46, 40–52.Google Scholar
  6. 6.
    Davis, B. J. (1964) Disc Electrophoresis-II: Method and application to human serum proteins. Ann. NY Acad. Sci. 15, 404–427.Google Scholar
  7. 7.
    D’Surney, S. J., Tschaplinski, T. J., Edwards, N. T., Shugart, L. R. (1993) Biological responses of two soybean cultivars exposed to enhanced UVB radiation. Environ. Exp. Bot. 33, 347–356.Google Scholar
  8. 8.
    Esterbauer, H., Cheeseman, K. (1990) Determination of aldehyde lipid peroxidation products: mal-onaldehyde and hydroxynonenal. Methods Enzymol. 186, 407–431.PubMedGoogle Scholar
  9. 9.
    Fedina, I., Georgieva, K., Grigorova, I. (2003) Response of barley seedlings to UV-B radiation as affected by proline and NaCl. Biol. Plant. 47, 549–554.Google Scholar
  10. 10.
    Fedina, I., Velitchkova, M, Georgieva, K., Grigorova, I. (2005) UV-B induced compounds as affected by proline and NaCl in Hordeum vulgare L. cv. Alfa. Environ. Exp. Bot. 54, 182–191.Google Scholar
  11. 11.
    Foyer, C. R., Lelandais, M., Kunert, K. (1994) Photooxidative stress in plants. Physiol. Plant. 92, 696–717.Google Scholar
  12. 12.
    Greneche, M., Lallemand, J., Michaud, O. (1991) Comparison of different enzyme loci as a means of distinguishing ryegrass varieties by electrophoresis. Seed Sci. Technol. 19, 147–158.Google Scholar
  13. 13.
    Hada, H., Hidema, J., Maekawa, M., Kumagai, T. (2003) Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.). Plant CellEnviron. 26, 1691–1701.Google Scholar
  14. 14.
    He, J., Huang, L.-K., Chow, W. S., Whitecross, M. I., Anderson, J. M. (1993) Effect of supplementary ultraviolet-B radiation on rice and pea plants. Aust. J. Plant Physiol. 20, 129–142.Google Scholar
  15. 15.
    Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stichiom-etry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 189–198.PubMedPubMedCentralGoogle Scholar
  16. 16.
    Hidema, J., Kyung Song, I. L., Sato, T., Kumagai, T. (2001) Relationship between ultraviolet-B sensitivity and cyclobutane pyrimidine dimmer photorepair in rice. J. Radiat. Res. 42, 295–303.PubMedGoogle Scholar
  17. 17.
    Jansen, M. A. K., Gaba, V., Greenberg, B. M. (1998) Higher plants and UV-B radiation: balancing damage, repair and acclimation. Trends Plant Sci. 3, 131–135.Google Scholar
  18. 18.
    Jansen, M. A. K., Gaba, V., Greenberg, B. M., Mattoo, A. K., Edelman, M. (1996) Low threshold levels of ultraviolet-B in a background of photosynthetically active radiation trigger rapid degradation of the D2 protein of photosystem II. Plant J. 9, 693–696.Google Scholar
  19. 19.
    Jordan, B. R. (1996) The effect of ultraviolet-B radiation on plants: a molecular perspective. Adv. Bot. Res. 22, 97–162.Google Scholar
  20. 20.
    Jordan, B. R., James, P. E., Strid, A., Anthony, R. G. (1994) The effect of ultraviolet-B radiation on gene expression and pigment composition in etiolated and green pea leaf tissue: UV-B-induced changes are gene-specific and dependent upon the developmental stage. Plant Cell Environ. 17, 5–54.Google Scholar
  21. 21.
    Kang, H. S., Hidema, J., Kumagai, T (1998) Effect of light environment during culture on UV-induced cyclobutyll pyrimidine dimmers and their photorepair in Rice (Oriza sativa L.). Photochem.Google Scholar
  22. 22.
    Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Natur. 227, 680–685.Google Scholar
  23. 23.
    Li, J., Ou-Lee, T. M., Raba, R. R. G., Amundson, R. G., Last, R. L. (1993) Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cel. 5, 171–179.Google Scholar
  24. 24.
    Lichtenthaler, H. K. (1987) Chlorophylls and carotenoids; pigments of photo synthetic biomembranes. Methods Enzymol. 148, 350–382.Google Scholar
  25. 25.
    Lowry, O. H., Rosenber, N. J., Farr, A. L., Randal, R. L. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
  26. 26.
    Mackerness, S. A.-H., Butt, J. P., Jordan, B. R. (1996) Amelioration of ultraviolet-B-induced down regulation of mRNA transcript for chloroplast proteins, by irradiance, is mediated by photosynthesis. J. Plant Physiol. 148, 100–106.Google Scholar
  27. 27.
    Mackerness, S. A.-H., Surplus, S. L., Jordan, B. R., Thomas, B. (1998) Effects of supplementary UV-B radiation on photo synthetic transcripts at different stages of leaf development and light levels in pea: role of active oxygen species and antioxidant enzymes. Photochem. Photobiol. 68, 88–96.Google Scholar
  28. 28.
    Mirecki, R. M., Teramura, A. H. (1984) Effects of ultraviolet-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol. 74, 475–480.PubMedPubMedCentralGoogle Scholar
  29. 29.
    Negash, L., Björn, L. O. (1986) Stomatal closure by ultraviolet radiation. Physiol. Plant. 66, 360–364.Google Scholar
  30. 30.
    Nesterenko, M. V., Tilley, M., Upton, J. (1994) A simple modification of Blum’s silver stain method allows for 30 minute detection of proteins in Polyacrylamide gels. J. Biochem. Biophys. Method. 28, 239–242.Google Scholar
  31. 31.
    Ornstein, L. (1964) Enzyme Bulletin Canalco Industrial Corporation. Rockvill, Maryland.Google Scholar
  32. 32.
    Radyuk, M. S., Homan, N. M. (2002) Discrete character of the development of the photosynthetic apparatus in greening barley leaves. Photosynth. Res. 72, 117–122.PubMedGoogle Scholar
  33. 33.
    Rajagopal, S., Sicora, C., Várkonyi, Z., Mustárdy, L., Mohanty, P. (2005) Protective effect of supplemental low intensity white light on ultraviolet-B exposure-induced impairment in cyanobacterium Spirulina platensis: Formation of air vacuoles as a possible protective measure. Photosynth. Res. 85, 181–189.PubMedGoogle Scholar
  34. 34.
    Rao, M. V., Paliyathy, G., Ormrod, D. P. (1996) Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol. 110, 125–136.PubMedPubMedCentralGoogle Scholar
  35. 35.
    Rathore, D., Agrawa, S. B., Singh, A. (2003) Influence of supplemental UV-B radiation and mineral nutrients on biomass, pigments and yield of two cultivars of wheat (Triticum aestivum L.). Int. J. Biotronik. 32, 1–15.Google Scholar
  36. 36.
    Ries, G., Heller, W., Puchta, H., Sandermann, H., Seidlitz, H. K., Hohn, B. (2000) Elevated UV-B radiation reduces genome stability in plants. Natur. 406, 98–101.Google Scholar
  37. 37.
    Smirnoff, N. (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 125, 27–58.Google Scholar
  38. 38.
    Schreiber, U., Schliwa, U., Bilger, W. (1986) Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62.Google Scholar
  39. 39.
    Searles, P. S., Flint, S. D., Caldwell, M. M. (2001) A meta-analysis of plant studies simulating stratospheric ozone depletion. Oecologi. 127, 1–10.Google Scholar
  40. 40.
    Sharma, P. K., Anand, P., Sankhalkar, S. (1998) Oxidative damage and changes in activities of antioxidant enzymes in wheat seedlings exposed to ultraviolet-B radiation. Curr Sci. 75, 359–366.Google Scholar
  41. 41.
    Strid, Å. (1993) Increased expression of defence genes in Pisum sativum after exposure to supplementary ultraviolet-B radiation. Plant Cell Physiol. 34, 949–953.Google Scholar
  42. 42.
    Strid, Å., Chow, W. S., Anderson, J. M. (1990) Effects of supplementary ultraviolet-B radiation on photosynthesis of in Pisum sativum. Biochim. Biophys. Act. 1020, 260–268.Google Scholar
  43. 43.
    Takeuchi, A., Yamaguchi, T., Hidema, J., Strid, A., Kumagai, T (2002) Changes in synthesis growing under supplementary UV-B radiation. Plant Cell Environ. 25, 695–705.Google Scholar
  44. 44.
    Tekchandani, K., Guruprasad, N. (1998) Modulation of guaiacol peroxidase inhibitor by UV-B in cucumber cotyledons. Plant Sci. 136, 131–137.Google Scholar
  45. 45.
    Tevini, M., Iwanzik, W., Thoma, U. (1981) Some effects of enhanced UV-B radiation on the growth and composition of plants. Plant. 153, 388–394.Google Scholar
  46. 46.
    Tullberg, A., Alexciev, K., Pfannschmidt, T., Allen, J. F. (2000) Photosynthetic electron flow regulates transcription of the psaB gene in pea (Pisum sativum L.) chloroplasts through the redox state of the plastoquinine pool. Plant Cell Physiol. 41, 1045–1054.PubMedGoogle Scholar
  47. 47.
    Tyystjarvi, E., Karunen, J. (1990) A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients. Photosynth. Res. 26, 127–132.PubMedGoogle Scholar
  48. 48.
    Willekens, H., Van Camp, W., Van Montagu, M., Inze, D., Langebartels, C., Sandermann, H. (1994) Ozone, sulfur dioxide, and ultraviolet-B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L. Plant Physiol. 106, 1007–1014.PubMedPubMedCentralGoogle Scholar
  49. 49.
    Woodbury, W., Spenser, A. K., Stahmann, M. A. (1971) An improved procedure using ferricyanide for detecting catalase isoenzymes. Anal. Biochem. 44, 301–305.PubMedPubMedCentralGoogle Scholar

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© Akadémiai Kiadó, Budapest 2009

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ivanka Fedina
    • 1
    Email author
  • Maya Velitchkova
    • 2
  • Katya Georgieva
    • 1
  • Dimitrina Nedeva
    • 1
  • H. Çakirlar
    • 3
  1. 1.Department Plant Stress Molecular Biology, Academic Metodi Popov Institute of Plant PhysiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of Photoexcitable MembranesInstitute of Biophysics, Bulgarian Academy of SciencesSofiaBulgaria
  3. 3.Department of BiologyHacettepe UniversityBeytepe, AnkaraTurkey

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