Comparison of Murine Fibroblast Cell Response to Fluor-Hydroxyapatite Composite, Fluorapatite And Hydroxyapatite By Eluate Assay


Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetic composite that contains the same molecular concentration of OH groups and F ions. The aim of this experiment was to evaluate the cellular responses of murine fibroblast NIH-3T3 cells in vitro to solid solutions of FHA and FA and to compare them with the effect of hydroxyapatite (HA). We studied 24, 48 and 72 h effects of biomaterials on cell morphology, proliferation and cell cycle of NIH-3T3 cells by eluate assay. Furthermore, we examined the ability of FHA, FA and HA to induce cell death and DNA damage. Our cytotoxic/antiproliferative studies indicated that any of tested biomaterials did not cause the total inhibition of cell division. Biomaterials induced different antiproliferative effects increasing in the order HA < FHA < FA which were time- and concentration-dependent. None of the tested biomaterials induced necrotic/apoptotic death of NIH-3T3 cells. On the other hand, after 72 h we found that FHA and FA induced G0/G1 arrest of NIH-3T3 cells, while HA did not affect any cell cycle phases. Comet assay showed that while HA demonstrated weaker genotoxicity, DNA damage induced by FHA and FA caused G0/G1 arrest of NIH-3T3 cells. Fluoridation of hydroxyapatite and different FHA and FA structure caused different cell response of NIH-3T3 cells to biomaterials.


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This work was supported by Technology Assistance Agency under the contract No. APVT 20-015904. The authors would like to thank Dr. Mira Horváthová for the opportunity to measure cell cycle profiles.

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Correspondence to Silvia Letašiová.

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Jantová, S., Letašiová, S., Theiszová, M. et al. Comparison of Murine Fibroblast Cell Response to Fluor-Hydroxyapatite Composite, Fluorapatite And Hydroxyapatite By Eluate Assay. BIOLOGIA FUTURA 60, 89–107 (2009).

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  • Fluor-hydroxyapatite composite
  • fluorapatite
  • cytotoxicity/genotoxicity
  • cell death
  • cell cycle