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Acta Biologica Hungarica

, Volume 59, Supplement 2, pp 227–235 | Cite as

Neuropeptides and Their Functions in Hydra

  • T. Takahashi
  • E. Hayakawa
  • O. Koizumi
  • T. FujisawaEmail author
Article

Abstract

In order to identify novel peptide signaling molecules involved in the regulation of developmental and physiological processes in the freshwater cnidarian, Hydra magnipapillata, we initiated a systematic peptide screening project, the Hydra Peptide Project. In the project, twelve neuropeptides were identified so far. The LWamide family is composed of seven members, which share a GLWamide motif at their C-termini. All the peptides have an ability to induce metamorphosis of Hydractinia serrata planula larvae into polyps. In Hydra, LWamides induce detachment of the bud from a parental polyp. A neuropeptide, Hym- 355, enhances neuronal differentiation by inducing the multipotent interstitial stem cells to enter the neuron differentiation pathway. A myoactive neutopeptide, Hym-176, specifically and reversibly induces contraction of the ectodermal muscle of the body column, in particularly in the peduncle region of epithelial Hydra that totally lack nerve cells. Two members of a novel neuropeptide family (FRamides) were contained in the same precursor. However, they have opposite myoactive functions in epithelial hydra. From these results, it seems fair to say reasonable to conclude that the so-called ‘primitive’ nervous system of Hydra is in reality more complex than generally believed.

Keywords

Neuropeptide metamorphosis neuron differentiation myoactivity Hydra 

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References

  1. 1.
    Bode, H. R. (1992) Continuous conversion of neuron phenotype in Hydra. Trends Genet.. 8, 279–284.CrossRefGoogle Scholar
  2. 2.
    Darmer, D., Schmutzler, C., Diekhoff, D., Grimmelikhuijzen, C. J. P. (1991) Primary structure of the precursor for the sea anemone neuropeptide Antho-RFamide (<Glu-Gly-Arg-Phe-NH2). Proc. Natl. Acad. Sci. USA. 88, 2555–2559.CrossRefGoogle Scholar
  3. 3.
    Darmer, D., Hauser, F., Nothacker, H.-P., Bosch, T. C. G., Williamson, M., Grimmelikhuijzen, C. J. P. (1998) Three different prohormones yield a variety of Hydra-RFamide (Arg-Phe-NH2) neuropeptides in Hydra magnipapillata. Biochem. J.. 332, 403–412.CrossRefGoogle Scholar
  4. 4.
    David, C. N. (1973) A quantitative method for maceration of Hydra tissue. Wilhelm Roux’s Arch. Entw. Mech. Org.. 171, 259–268.CrossRefGoogle Scholar
  5. 5.
    Dunne, J., Javois, L. C., Huang, L. W., Bode, H. R. (1985) A subset of cells in the nerve net of Hydra oligactis defined by a monoclonal antibody: Its arrangement and development. Dev. Biol.. 109, 41–53.CrossRefGoogle Scholar
  6. 6.
    Grens, A., Gee, L., Fisher, D. A., Bode, H. R. (1996) CnNK-2, an NK-2 homeobox gene, has a role in patterning the basal end of the axis in Hydra. Dev. Biol.. 180, 473–488.CrossRefGoogle Scholar
  7. 7.
    Leitz, T., Morand, K., Mann, M. (1994) Metamorphosin A: A novel peptide controlling development of the lower metazoan Hydractinia echinata (Coelenterata, Hydrozoa). Dev. Biol.. 163, 440–446.CrossRefGoogle Scholar
  8. 8.
    Grimmelikhuijzen, C. J. P., Dockray, G. J., Schot, L. P. C. (1982a) FMRFamide-like immunoreactivity in the nervous system of Hydra. Histochemistry. 73, 499–508.CrossRefGoogle Scholar
  9. 9.
    Grimmelikhuijzen, C. J. P., Dierickx, K., Boer, G. J. (1982b) Oxytocin/vasopressin-like immunoreactivity in the nervous system of Hydra. Neurosci.. 7, 3191–3199.CrossRefGoogle Scholar
  10. 10.
    Koizumi, O., Heimfeld, S., Bode, H. R. (1988) Plasticity in the nervous system of adult Hydra. II. Conversion of ganglion cells of the body column into epidermal sensory cells of the hypostome. Dev. Biol.. 129, 358–371.CrossRefGoogle Scholar
  11. 11.
    Leviev, I., Grimmelikhuijzen, C. J. P. (1995) Molecular cloning of a preprohormone from sea anemones containing numerous copies of a metamorphosis inducing neuropeptide: a likely role for dipeptidyl aminopeptidase in neuropeptide precursor processing. Proc. Natl. Acad. Sci. USA. 92, 11647–11651.CrossRefGoogle Scholar
  12. 12.
    Leviev, I., Williamson, M., Grimmelikhuijzen, C. J. P. (1997) Molecular cloning of a preprohormone from Hydra magnipapillata containing multiple copies of Hydra-LWamide (Leu-Trp-NH2) neuropeptides: evidence for processing at Ser and Asn residues. J. Neurochem.. 68, 1319–1325.CrossRefGoogle Scholar
  13. 13.
    Sugiyama, T., Fujisawa, T. (1977) Genetic analysis of developmental mechanisms in Hydra. I. Sexual reproduction of Hydra magnipapillata and isolation of mutants. Dev. Groth Differ.. 19, 187–200.CrossRefGoogle Scholar
  14. 14.
    Sugiyama, T., Fujisawa, T. (1978) Genetic analysis of developmental mechanisms in Hydra. II. Isolation and characterization of an interstitial cell-deficient strain. J. Cell. Sci.. 29, 35–52.PubMedGoogle Scholar
  15. 15.
    Takahashi, T., Muneoka, Y., Lohmann, J., deHaro, L. M., Solleder, G., Bosch, T. C. G., David, C. N., Bode, H. R., Koizumi, O., Shimizu, H., Hatta, M., Fujisawa, T., Sugiyama, T. (1997) Systematic isolation of peptide signal molecules regulating development in Hydra: LWamide and PW families. Proc. Natl. Acad. Sci. USA. 94, 1241–1246.CrossRefGoogle Scholar
  16. 16.
    Takahashi, T., Koizumi, O., Ariura, Y., Romanovitch, A., Bosch, T. C. G., Kobayakawa, Y., Mohri, S., Bode, H. R., Yum, S., Hatta, M., Fujisawa, T. (2000) A novel neuropeptide, Hym-355, positively regulates neuron differentiation in Hydra. Development. 127, 997–1005.PubMedGoogle Scholar
  17. 17.
    Takahashi, T., Kobayakawa, Y., Muneoka, Y., Fujisawa, Y., Mohri, S., Hatta, M., Shimizu, H., Fujisawa, T., Sugiyama, T., Takahara, M., Yanagi, K., Koizumi, O. (2003) Identification of a new member of the GLWamide peptide family: Physiological activity and cellular localization in cnidarian polyps. Com. Biochem. Physiol. B. 135, 309–324.CrossRefGoogle Scholar
  18. 18.
    Yum, S., Takahashi, T., Koizumi, O., Ariura, Y., Kobayakawa, Y., Mohri, S., Fujisawa, T. (1998) A novel neuropeptide, Hym-176, induces contraction of the ectodermal muscle in Hydra. Biochem. Biophys. Res. Comm.. 248, 584–590.CrossRefGoogle Scholar
  19. 19.
    Yum, S., Takahashi, T., Hatta, M., Fujisawa, T. (1998) The structure and expression of a preprohormone of a neuropeptide, Hym-176 in Hydra magnipapillata. FEBS Letters. 439, 31–34.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • T. Takahashi
    • 1
  • E. Hayakawa
    • 2
  • O. Koizumi
    • 3
  • T. Fujisawa
    • 2
    Email author
  1. 1.Suntory Institute for Bioorganic ResearchOsakaJapan
  2. 2.National Institute of GeneticsMishima, ShizuokaJapan
  3. 3.Fukuoka Women’s UniversityFukuokaJapan

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