Immunohistological Studies on the Distribution of Learning-Related Peptides in the Central Nervous System of Conditioned Lymnaea
Abstract
Behavioral conditioning in Lymnaea increased the amount of immunolabeling in the central nervous system for the memory-associated protein calexcitin. The staining level of anti-calexcitin positive neurons was always stronger in conditioned animals than in naïve animals. In the visuo-vestibular conditioned animals, right-parietal and visceral group neurons as well as withdrawal-related neurons were positively stained with anti-calexcitin antibody. In taste-aversion conditioned animals, right-parietal visceral G-group neurons and withdrawal-related neurons were selectively stained. These neurons are candidate neurons for modulation by these conditioning paradigms.
Keywords
Calexcitin visuo-vestibular conditioning taste-aversion conditioning immunohistochemistry whole-body withdrawal responseReferences
- 1.Alkon, D. L. (1987) Memory Traces in the Brain. Cambridge University Press, Cambridge, London, New York, Rochelle, Melbourne, Sydney.Google Scholar
- 2.Alkon, D. L., Nelson, T. J. (1990) Specificity of molecular changes in neurons involved in memory storage. Faseb. J. 4, 1567–1576.CrossRefGoogle Scholar
- 3.Alkon, D. L., Nelson, T. J., Zhao, W., Cavallaro, S. (1998) Time domains of neuronal Ca2+ signaling and associative memory: steps through a calexcitin, ryanodine receptor, K+ channel cascade. Trends. Neurosci. 21, 529–537.CrossRefGoogle Scholar
- 4.Ascoli, G. A., Luu, K. X., Olds, J. L., Nelson, T. J., Gusev, P. A., Bertucci, C., Bramanti, E., Raffaelli, A., Salvadori, P., Alkon, D. L. (1997) Secondary structure and Ca2+-induced conformational change of calexcitin, a learning-associated protein. J. Biol. Chem. 272, 24771–24779.CrossRefGoogle Scholar
- 5.Elekes, K., Eckert, M., Rapus, J. (1993) Small sets of putative interneurons are octopamineimmunoreactive in the central nervous system of the pond snail, Lymnaea stagnalis. Brain. Res. 608, 191–197.CrossRefGoogle Scholar
- 6.Ferguson, G. P., Benjamin, P. R. (1991) The whole-body withdrawal response of Lymnaea stagnalis. I. Identification of central motoneurones and muscles. J. Exp. Biol. 158, 63–95.PubMedGoogle Scholar
- 7.Hatakeyama, D., Inamura, S., Ito, E., Sakakibara, M., Nelson, T. J., Alkon, D. L. (2004) Calexcitinlike immunoreactivity in the pond snail Lymnaea stagnalis. Neurosci. Res. Comm. 35, 32–40.CrossRefGoogle Scholar
- 8.Kawai, R., Sunada, H., Horikoshi, T., Sakakibara, M. (2004) Conditioned taste aversion with sucrose and tactile stimuli in the pond snail Lymnaea stagnalis. Neurobiol. Learn. Mem. 82, 164–168.CrossRefGoogle Scholar
- 9.Kuzirian, A. M., Epstein, H. T., Buck, D., Child, F. M., Nelson, T., Alkon, D. L. (2001) Pavlovian conditioning-specific increases of the Ca2+- and GTP-binding protein, calexcitin in identified Hermissenda visual cells. J. Neurocytol. 30, 993–1008.CrossRefGoogle Scholar
- 10.Kuzirian, A. M., Epstein, H. T., Gagliardi, C. J., Nelson, T. J., Sakakibara, M., Taylor, C., Scioletti, A. B., Alkon, D. L. (2006) Bryostatin enhancement of memory in Hermissenda. Biol. Bull. 210, 201–214.CrossRefGoogle Scholar
- 11.Nelson, T. J., Alkon, D. L. (1995) Phosphorylation of the conditioning-associated GTP-binding protein cp20 by protein kinase C. J. Neurochem. 65, 2350–2357.CrossRefGoogle Scholar
- 12.Ono, M., Kawai, R., Horikoshi, T., Yasuoka, T., Sakakibara, M. (2002) Associative learning acquisition and retention depends on developmental stage in Lymnaea stagnalis. Neurobiol. Learn. Mem. 78, 53–64.CrossRefGoogle Scholar
- 13.Ridgway, R. L., Syed, N. I., Lukowiak, K., Bulloch, A. G. (1991) Nerve growth factor (NGF) induces sprouting of specific neurons of the snail, Lymnaea stagnalis. J. Neurobiol. 22, 377–390.CrossRefGoogle Scholar
- 14.Sakakibara, M. (2006) Comparative study of visuo-vestibular conditioning in Lymnaea stagnalis. Biol. Bull. 210, 298–307.CrossRefGoogle Scholar
- 15.Sakakibara, M., Aritaka, T., Iizuka, A., Suzuki, H., Horikoshi, T., Lukowiak, K. (2005) Electrophysiological responses to light of neurons in the eye and statocyst of Lymnaea stagnalis. J. Neurophysiol. 93, 493–507.CrossRefGoogle Scholar
- 16.Sakakibara, M., Kawai, R., Kobayashi, S., Horikoshi, T. (1998) Associative learning of visual and vestibular stimuli in Lymnaea. Neurobiol. Learn. Mem. 69, 1–12.CrossRefGoogle Scholar
- 17.Sakakibara, M., Okuda, F., Nomura, K., Watanabe, K., Meng, H., Horikoshi, T., Lukowiak, K. (2005) Potassium currents in isolated statocyst neuron and RPeD1 in the pond snail, Lymnaea stagnalis. J. Neurophysiol. 94, 3884–3892.CrossRefGoogle Scholar
- 18.Syed, N. I., Winlow, W. (1991) Coordination of locomotor and cardiorespiratory networks of Lymnaea stagnalis by a pair of identified interneurones. J. Exp. Biol. 158, 37–62.Google Scholar
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