The changes in the polypeptide patterns of soluble proteins of wheat flag leaves after treatment with two synthetic cytokinin-like substances 3-CP-2-PU [1-(3-chlorophenyl)-3-(2-pyridyl)urea] and 3-CP-4-MPU [1-(3-chlorophenyl)-3-(4-methyl-2-pyridyl)urea] was studied. Both compounds increased differentially in quantity and composition most individual polypeptides identified compared to the non-treated leaves. While 3-CP-2-PU increased only the polypeptide quantity, 3-CP-4-MPU led to the appearance of new polypeptide with Mw of 51 kDa.
Badger, M. R., Price, G. D. (1994) The role of carbonic anhydrase in photosynthesis. Ann. Rev. Plant Physiol. Plant Mol. Biol. 45, 369–392.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Brinegar, A. C., Fox, J. E. (1985) Resolution of the subunit composition of a cytokinin-binding protein from wheat embryos. Biol. Plant 27, 100–104.
Chernyad’ev, I., Monakhova, O. (2003) Effect of cytokinin preparations on the pools of pigments and proteins of wheat cultivars differing in their tolerance to water stress. Prikl. Biokhim. Mikrobiol. 339, 593–601.
Demirevska-Kepova, K. (2002) Ribulose-1,5-bisphosphate carboxylase/oxigenase (RUBISCO), binding protein and Rubisco activase from Hordeum vulgare L. - Immunochemical characteristic and protein interactions. DSci. Thesis, Sofia, pp. 62.
Kaminek, M, Trckova, M., Fox, J. E., Gaudinova, A. (2003) Comparison of cytokinin-binding proteins from wheat and oat grains. Physiol. Plant 117, 453–458.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Popova, L., Kolev, K., Stoinova, J. (1994) Abscisic acid induced changes in the polypeptide patterns of the soluble leaf proteins in barley plants. Compt. Rend. Bulg. Acad. Sci. 47, 77–80.
Romanov, G. A., Taran, V. Ya., Chvojka, L., Kulaeva, O. N. (1986) Specific binding of a zeatin to a protein fraction of barley leaves and purification of cytokinin-binding proteins. Soviet Plant Physiol. 33, 93–104.
Salvucci, M. E., Werneke, J. M., Ogren, W. L., Portis, A. R. (1987) Participation and species distribution of Rubisco activase. Plant Physiol. 84, 930–936.
Stefanov, B. J., Iliev, L. K., Popova, N. I. (1994) Effect of the phenylurea cytokinin 4PU-30 on the growth and protein composition of maize seedlings. J. Plant Growth Regul. 13, 231–234.
Stefanov, B. J., Iliev, L. K., Popova, N. I. (1998) Influence of GA3 and 4PU-30 on leaf protein composition, photo synthetic activity and growth of maize seedlings. Biol. Plant 41, 57–63.
Sugiharto, B., Burnell, J. N., Sugiyama, T (1992) Cytokinin is required to induce the nitrogen-dependent accumulation of mRNAs for phosphoenolpiruvate carboxylase and carbonic anhydrase in detached maize leaves. Plant Physiol. 100, 153–156.
Tsenova, E. N. (1988) Stimulating effect of some phenylurea cytokinins on the NADP-glyceralde-hyde-3-phosphate dehydrogenase activity and other photo synthetic parameters in barley plants. In: Vaklinova, S., Stanev, V., Dilova, M. (eds) Plant Mineral Nutrition and Photosynthesis, Vol. II, Sofia, Bulgaria, pp. 365–369.
Yonova, P. A., Vassilev, G. N. (1987) Chemical structure and biological activity of N-(3- and 4-chlorophenyl)-N’-(pyridyl and methylpyridyl)ureas. In: Lilov, D., Vassilev, G., Christov, Chr., Andonova, T. (eds) Plant Growth Regulators. Publishing House of the BAS, Sofia, pp. 407–412.
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Lazova, G., Yonova, P. Changes in the Leaf Polypeptide Patterns of Wheat Flag Leaves Spraying with Non-Purine Cytokinins. BIOLOGIA FUTURA 59, 501–505 (2008). https://doi.org/10.1556/ABiol.59.2008.4.10
- Triticum aestivum
- cytokinin-like substances
- leaf protein
- polypeptide patterns