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Acta Biologica Hungarica

, Volume 59, Issue 3, pp 289–304 | Cite as

Purification and Characterization of a Thermal Stable Antimicrobial Protein From Housefly Larvae. Musca Domestica, Induced by Ultrasonic Wave

  • Y. J. HaoEmail author
  • Y. J. Jing
  • H. Qu
  • D. S. Li
  • R. Q. Du
Article

Abstract

This work describes the induction, purification and partial biochemical characterizations of an antimicrobial protein from the housefly larvae induced by ultrasonic wave. It has been purified to apparent homogeneity by ammonium sulfate precipitation followed by Sephadex G-75, Bio-gel P6 gel filtration, and CM-Sepharose Fast Flow cation exchange chromatography. The protein is a cationic protein with an apparent molecular weight of 16315 Da determined by no-denaturing electrophoresis and SDS-PAGE, respectively. Biochemical profile assays show that this protein has good thermal stability, and repeatedly frozen and defrosted durability. The optimum pH for antimicrobial activity is around pH5. The antimicrobial range of the protein includes Gram-positive, Gram-negative bacteria and some fungi. Results of the membrane permeability assays suggest that the probable mode of action of this protein is membrane-disrupting mechanism.

Keywords

Housefly Musca domestica antimicrobial protein antimicrobial activity membrane permeability 

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Y. J. Hao
    • 1
    • 3
    Email author
  • Y. J. Jing
    • 2
  • H. Qu
    • 1
  • D. S. Li
    • 1
  • R. Q. Du
    • 1
  1. 1.Department of Cell and Genetics, Genetic Engineering LaboratoryCollege of Life Science, Nankai UniversityTian JinChina
  2. 2.Department of Bioengineering, College of Chemical EngineeringHebei University of TechnologyTian JinChina
  3. 3.Departamento de BiologiaUniversidade dos AcoresPonta Delgada, AzoresPortugal

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