Boric Acid-Induced Effects on Protein Profiles of Galleria Mellonella Hemolymph and Fat Body

Abstract

The dietary effects of boric acid (BA) on the protein profiles of greater wax moth. Galleria mello-nella (L.), were investigated in hemolymph and fat body of final instar (VIIth) and pupae. The insects were reared from first-instar larvae on an artificial diets containing 156, 620, 1250 or 2500 ppm of BA. We detected many undetermined protein fractions (6.5–260 kDa) in addition to well-defined protein fractions such as lipophorins and storage proteins in the tissues by using sodium dodecyl-sulphate Polyacrylamide gradient gel electrophoresis. A marked quantitative change in the 45 kDa protein fraction of the hemolymph was observed in the Vllth instar larvae reared on 2500 ppm dietary BA.

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Correspondence to P. Hyršl.

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Hyršl, P., Büyükgüzel, E. & Büyükgüzel, K. Boric Acid-Induced Effects on Protein Profiles of Galleria Mellonella Hemolymph and Fat Body. BIOLOGIA FUTURA 59, 281–288 (2008). https://doi.org/10.1556/ABiol.59.2008.3.2

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Keywords

  • Galleria mellonella
  • boric acid
  • protein profiles
  • hemolymph
  • fat body