Selection of Salt Tolerant Plants of Nicotiana Tabacum L. Through in vitro and its Biochemical Characterization
Abstract
Sodium chloride tolerant organogenic callus lines of Nicotiana tabacum were developed in vitro on Murashige and Skoog [16] medium supplemented with BA, IAA and different concentration of NaCl. The maximum shoot bud regeneration was achieved from both tolerant and non-tolerant calluses on MS medium supplemented with 1.0 mg/1 BA, 0.1 mg/1 IAA with or without NaCl within 4 weeks of culture. Standard growth parameters such as fresh weight and dry weight of organogenic callus, growth tolerant index and enzyme activity (peroxidase and catalase) were used as indicators of salt tolerance. The growth tolerance index in the 4-week after the beginning of treatments yielded significant differences among the non-tolerant and tolerant organogenic callus lines. The regenerated shoots were rooted on half-strength MS basal salts supplemented with 2% sucrose but devoid of growth regulator. The regenerated plants from tolerant callus lines were capable of growing in vitro in presence of 175 niM NaCl. SDS-PAGE profile showed that the progenies derived from tolerant sources were tolerant to salt. This investigation may help in the selection and characterization of salt tolerance in plant improvement programme.
Keywords
Enzyme activity In vitro culture N. tabacum salt tolerancePreview
Unable to display preview. Download preview PDF.
References
- 1.Ashraf M. (1994) Organic substances responsible for salt tolerance in Eruca sattva. Biol. Plant. 36, 255–259.CrossRefGoogle Scholar
- 2.Ashraf M. (2002) Salt tolerance of cotton: some new advances. Crit. Rev. Plant Sci. 21, 1–30.CrossRefGoogle Scholar
- 3.Ashraf M., Tufail M. (1995) Variation in salinity tolerance in sunflower (Helianthus annus L.). J. Agronom. Soil Sci. 174, 351–362.Google Scholar
- 4.Ashraf M., Harris P. J. C. (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166, 3–16.CrossRefGoogle Scholar
- 5.Basu S., Gangopadhyay G., Mukherjee B. B., Gupta S. (1997) Plant regeneration of salt adapted callus of indicarice (var. Basumati 370) in saline conditions. Plant Cell Tiss. Org. Cult. 50, 153–159.CrossRefGoogle Scholar
- 6.Binh D. Q., Heszky L. E., Gyulai G., Csillag A. (1992) Plant regeneration of NaCl-pretreated cells from long term suspension culture of rice (Oryza sativa L.) in high saline conditions. Plant Cell Tiss. Org. Cult. 29, 75–82.CrossRefGoogle Scholar
- 7.Cushman, J. C., De Rocher E. J., Bohnert H. J. (1990) Gene expression during adaptation to salt stress. In: Katterman F. (ed.) Environmental Injury to Plants. Academic Press, San Diego, USA, pp. 173–203.CrossRefGoogle Scholar
- 8.Hasegawa P. M., Bressan, R. A., Handa A. K. (1980) Growth characteristics of NaCl selected and non-selected cells of Nicotiana tabacum. Plant Cell Physiol. 21, 1347–1355.CrossRefGoogle Scholar
- 9.Hasegawa P. M., Bressan R. A., Zhu J. K., Bohnert H. J. (2000) Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Physiol. Plant Mol. Biol. 51, 463–499.CrossRefGoogle Scholar
- 10.Heszky L. E., Li S. N., Horvath Z. S. (1986) Rice tissue culture and application to breeding.II. Factors affecting the plant regeneration during subculture of diploid and haploid callus. Cereal Res. Comm. 14, 289–296.Google Scholar
- 11.Hurkman W. J., Fomari C. S., Tanaka C. K. (1989) A comparison of the effect of salt on polypeptide and translatable mRNA in roots of a salt tolerant and salt sensitive cultivar of barley. Plant Physiol. 90, 1444–1456.CrossRefGoogle Scholar
- 12.Iyengar E. R. R., Reddy M. P. (1996) Photosynthesis in high salt tolerant plants. In: Pesserkali M. (ed.) Hand Book of Photosynthesis. Marshal Dekker, Baton Rouge, LA, USA, pp. 56–65.Google Scholar
- 13.Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. (1951) Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
- 14.Lutts S., Kinet J. M., Bouharmont J. (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sati L.) cultivars differing in salinity tolerance. Plant Growth Regul. 19, 207–218.CrossRefGoogle Scholar
- 15.Marascuilo L. A., McSweeney M. (1977) Non-parametric and distribution free methods for the Social Sciences. Books/Cole Publ. Co., California, USA, pp. 141–147.Google Scholar
- 16.Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
- 17.Noble C. L., Halloran G. M., West D. W. (1984) Identification and selection for salt tolerance in lecerne (Medicago sativa L.). Aust. J. Agric. Res. 35, 239–252.CrossRefGoogle Scholar
- 18.Noble C. L., Rogers M. E. (1992) Arguments for the use of physiological criteria for improving the salt tolerance in crops. Plant Soil. 146, 99–107.CrossRefGoogle Scholar
- 19.Parida A., Das A. B. (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology andEnviron. Safety 60, 324–349.CrossRefGoogle Scholar
- 20.Parida A., Das A. B., Das P. (2002) NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic compounds in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. J. Plant Biol. 45, 28–36.CrossRefGoogle Scholar
- 21.Reddy P. J., Vaidyanath K. (1986) In vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (Oryza sativa L.). Theor Appl. Genet. 71, 757–760.CrossRefGoogle Scholar
- 22.Shannon M. C. (1998) Adaptation of plants to salinity. Adv. Agron. 60, 75–119.CrossRefGoogle Scholar
- 23.Shah S. H., Wainwright S. J., Merett M. J. (1993) Cation co-tolerance in callus cultures of Medicago sativa L. tolerant to sodium chloride. Plant Sci. 89, 81–84.CrossRefGoogle Scholar
- 24.Tal M. (1983) Selection of stress tolerance. In: Evans D. A., Sharp W. R., Ammirato P. V., Yamada Y. (eds) Handbook of Plant Cell Culture. Vol. 1. MacMillan Inc., New York, USA, pp. 461–488.Google Scholar
- 25.Uma S., Prasad T. G., Kumar M. U. (1995) Genetic variability in recovery growth and synthesis of stress proteins in response to polyethylene glycol and salt stress in finger millet. Ann. Bot. 76, 43–49.CrossRefGoogle Scholar
- 26.Vernon L. P. (1960) Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. Anal. Chem. 32, 1144–1150.CrossRefGoogle Scholar
- 27.Watad A. A., Reinhold L., Lernes M. (1983) Comparison between a stable NaCl-selected Nicotiana cell line and wild type. Plant Physiol. 73, 629–642.CrossRefGoogle Scholar
Copyright information
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.