Acta Biologica Hungarica

, Volume 59, Issue 1, pp 77–92 | Cite as

Selection of Salt Tolerant Plants of Nicotiana Tabacum L. Through in vitro and its Biochemical Characterization

  • G. R. RoutEmail author
  • S. K. Senapati
  • Jiban Jyoti Panda


Sodium chloride tolerant organogenic callus lines of Nicotiana tabacum were developed in vitro on Murashige and Skoog [16] medium supplemented with BA, IAA and different concentration of NaCl. The maximum shoot bud regeneration was achieved from both tolerant and non-tolerant calluses on MS medium supplemented with 1.0 mg/1 BA, 0.1 mg/1 IAA with or without NaCl within 4 weeks of culture. Standard growth parameters such as fresh weight and dry weight of organogenic callus, growth tolerant index and enzyme activity (peroxidase and catalase) were used as indicators of salt tolerance. The growth tolerance index in the 4-week after the beginning of treatments yielded significant differences among the non-tolerant and tolerant organogenic callus lines. The regenerated shoots were rooted on half-strength MS basal salts supplemented with 2% sucrose but devoid of growth regulator. The regenerated plants from tolerant callus lines were capable of growing in vitro in presence of 175 niM NaCl. SDS-PAGE profile showed that the progenies derived from tolerant sources were tolerant to salt. This investigation may help in the selection and characterization of salt tolerance in plant improvement programme.


Enzyme activity In vitro culture N. tabacum salt tolerance 


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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • G. R. Rout
    • 1
    Email author
  • S. K. Senapati
    • 1
  • Jiban Jyoti Panda
    • 1
  1. 1.Plant Biotechnology DivisionRegional Plant Resource CentreOrissaIndia

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