Sodium chloride tolerant organogenic callus lines of Nicotiana tabacum were developed in vitro on Murashige and Skoog  medium supplemented with BA, IAA and different concentration of NaCl. The maximum shoot bud regeneration was achieved from both tolerant and non-tolerant calluses on MS medium supplemented with 1.0 mg/1 BA, 0.1 mg/1 IAA with or without NaCl within 4 weeks of culture. Standard growth parameters such as fresh weight and dry weight of organogenic callus, growth tolerant index and enzyme activity (peroxidase and catalase) were used as indicators of salt tolerance. The growth tolerance index in the 4-week after the beginning of treatments yielded significant differences among the non-tolerant and tolerant organogenic callus lines. The regenerated shoots were rooted on half-strength MS basal salts supplemented with 2% sucrose but devoid of growth regulator. The regenerated plants from tolerant callus lines were capable of growing in vitro in presence of 175 niM NaCl. SDS-PAGE profile showed that the progenies derived from tolerant sources were tolerant to salt. This investigation may help in the selection and characterization of salt tolerance in plant improvement programme.
Ashraf M. (1994) Organic substances responsible for salt tolerance in Eruca sattva. Biol. Plant. 36, 255–259.
Ashraf M. (2002) Salt tolerance of cotton: some new advances. Crit. Rev. Plant Sci. 21, 1–30.
Ashraf M., Tufail M. (1995) Variation in salinity tolerance in sunflower (Helianthus annus L.). J. Agronom. Soil Sci. 174, 351–362.
Ashraf M., Harris P. J. C. (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166, 3–16.
Basu S., Gangopadhyay G., Mukherjee B. B., Gupta S. (1997) Plant regeneration of salt adapted callus of indicarice (var. Basumati 370) in saline conditions. Plant Cell Tiss. Org. Cult. 50, 153–159.
Binh D. Q., Heszky L. E., Gyulai G., Csillag A. (1992) Plant regeneration of NaCl-pretreated cells from long term suspension culture of rice (Oryza sativa L.) in high saline conditions. Plant Cell Tiss. Org. Cult. 29, 75–82.
Cushman, J. C., De Rocher E. J., Bohnert H. J. (1990) Gene expression during adaptation to salt stress. In: Katterman F. (ed.) Environmental Injury to Plants. Academic Press, San Diego, USA, pp. 173–203.
Hasegawa P. M., Bressan, R. A., Handa A. K. (1980) Growth characteristics of NaCl selected and non-selected cells of Nicotiana tabacum. Plant Cell Physiol. 21, 1347–1355.
Hasegawa P. M., Bressan R. A., Zhu J. K., Bohnert H. J. (2000) Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Physiol. Plant Mol. Biol. 51, 463–499.
Heszky L. E., Li S. N., Horvath Z. S. (1986) Rice tissue culture and application to breeding.II. Factors affecting the plant regeneration during subculture of diploid and haploid callus. Cereal Res. Comm. 14, 289–296.
Hurkman W. J., Fomari C. S., Tanaka C. K. (1989) A comparison of the effect of salt on polypeptide and translatable mRNA in roots of a salt tolerant and salt sensitive cultivar of barley. Plant Physiol. 90, 1444–1456.
Iyengar E. R. R., Reddy M. P. (1996) Photosynthesis in high salt tolerant plants. In: Pesserkali M. (ed.) Hand Book of Photosynthesis. Marshal Dekker, Baton Rouge, LA, USA, pp. 56–65.
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. (1951) Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193, 265–275.
Lutts S., Kinet J. M., Bouharmont J. (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sati L.) cultivars differing in salinity tolerance. Plant Growth Regul. 19, 207–218.
Marascuilo L. A., McSweeney M. (1977) Non-parametric and distribution free methods for the Social Sciences. Books/Cole Publ. Co., California, USA, pp. 141–147.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Noble C. L., Halloran G. M., West D. W. (1984) Identification and selection for salt tolerance in lecerne (Medicago sativa L.). Aust. J. Agric. Res. 35, 239–252.
Noble C. L., Rogers M. E. (1992) Arguments for the use of physiological criteria for improving the salt tolerance in crops. Plant Soil. 146, 99–107.
Parida A., Das A. B. (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology andEnviron. Safety 60, 324–349.
Parida A., Das A. B., Das P. (2002) NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic compounds in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. J. Plant Biol. 45, 28–36.
Reddy P. J., Vaidyanath K. (1986) In vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (Oryza sativa L.). Theor Appl. Genet. 71, 757–760.
Shannon M. C. (1998) Adaptation of plants to salinity. Adv. Agron. 60, 75–119.
Shah S. H., Wainwright S. J., Merett M. J. (1993) Cation co-tolerance in callus cultures of Medicago sativa L. tolerant to sodium chloride. Plant Sci. 89, 81–84.
Tal M. (1983) Selection of stress tolerance. In: Evans D. A., Sharp W. R., Ammirato P. V., Yamada Y. (eds) Handbook of Plant Cell Culture. Vol. 1. MacMillan Inc., New York, USA, pp. 461–488.
Uma S., Prasad T. G., Kumar M. U. (1995) Genetic variability in recovery growth and synthesis of stress proteins in response to polyethylene glycol and salt stress in finger millet. Ann. Bot. 76, 43–49.
Vernon L. P. (1960) Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. Anal. Chem. 32, 1144–1150.
Watad A. A., Reinhold L., Lernes M. (1983) Comparison between a stable NaCl-selected Nicotiana cell line and wild type. Plant Physiol. 73, 629–642.
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Rout, G.R., Senapati, S.K. & Panda, J.J. Selection of Salt Tolerant Plants of Nicotiana Tabacum L. Through in vitro and its Biochemical Characterization. BIOLOGIA FUTURA 59, 77–92 (2008). https://doi.org/10.1556/ABiol.59.2008.1.7
- Enzyme activity
- In vitro culture
- N. tabacum
- salt tolerance