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Acta Biologica Hungarica

, Volume 59, Issue 1, pp 57–66 | Cite as

Antioxidative and Metabolic Responses to Extended Cold Exposure in Rats

  • Sengul YukselEmail author
  • Dilek Asma
  • Ozfer Yesilada
Article

Abstract

In this work, we investigated whether extended cold exposure increases oxidative damage and susceptibility to oxidants of rat liver, heart, kidney and lung which are metabolically active tissues. Moreover in this study the effect of cold stress on some of the lipid metabolic mediators were studied in rat experimental model. Male albino Sprague-Dawley rats were randomly divided into two groups: The control group (n = 12) and the cold-stress group (n = 12). Tissue superoxide dismutase (SOD), catalase (CAT), glutathion S-transferase (GST) and glutathion reductase (GR) activities and glutathion (GSH) were measured using standard protocols. The biochemical analyses for total lipid, cholesterol, trigliceride, HDL, VLDL and LDL were done on autoanalyzer.

In cold-stress groups SOD activity was decreased in the lung whereas it increased in the heart and kidney. CAT activity was significantly decreased (except liver) in all the tissues in treated rats. GST activity of cold-induced rats increased in liver and heart while decreased in the lung. GR activity was significantly decreased (except in liver) in all the tissues in cold-stressed rats. GSH level was significantly increased in the heart but decreased in the lung of animals exposed to cold when compared to controls. It was found that among the groups trigliceride, total lipid, HDL and VLDL parameters varied significantly but cholesterol and LDL had no significant variance.

In this study, we found that exposure of extended (48 h) cold (8°C) caused changes both in the antioxidant defense system (as tissue and enzyme specific) and serum lipoprotein profiles in rats.

Keywords

Cold stress oxidative stress glutathione antioxidant enzymes lipid metabolism rat 

Abbreviations

CAT

catalase

GSH

glutathione

ROS

reactive oxygen species

GST

glutathione S-transferase

SOD

superoxide dismutase

GR

glutathione reductase

HDL

high-density lipoproteins

VLDL

very low density lipoproteins

LDL

low-density lipoproteins

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Medical Biology and GeneticsInonu Universty, School of MedicineMalatyaTurkey
  2. 2.Department of Molecular Biology, Science and Art FacultyInonu UniversityMalatyaTurkey

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