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Acta Biologica Hungarica

, Volume 58, Issue 2, pp 199–207 | Cite as

Cryopreservation of Sperm of Two European Percid Species, the Pikeperch (Sander Lucioperca) and the Volga Pikeperch (S. Volgensis)

  • Z. Bokor
  • T. Müller
  • M. Bercsényi
  • L. Horváth
  • B. Urbányi
  • Á. HorváthEmail author
Article

Abstract

Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis). Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cry-oprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1 : 1, 1 : 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21%) and fertilization rate (43 ± 12%) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22%) was observed with MeOH as cryoprotectant and 1: 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2%) did not significantly differ from the control (60 ± 6%). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.

Keywords

Pikeperch Volga pikeperch sperm cryopreservation urine 

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Notes

Acknowledgements

This work was supported by the OTKA T 048289, NKFP 4/006/2004, PÁL OM-28345/2003 and RET 12/05 projects as well as the Bolyai János Fellowship of the Hungarian Academy of Sciences.

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Z. Bokor
    • 1
  • T. Müller
    • 1
    • 2
  • M. Bercsényi
    • 2
  • L. Horváth
    • 1
  • B. Urbányi
    • 1
  • Á. Horváth
    • 1
    Email author
  1. 1.Department of Fish CultureSzent István UniversityGödöllőHungary
  2. 2.Georgikon Faculty of AgriculturePannon UniversityKeszthelyHungary

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