Acta Biologica Hungarica

, Volume 58, Issue 1, pp 87–92 | Cite as

Response of Inoculated Foliar Fed Pea Plants (Pisum sativum L.) to Reduced Mo Supply

  • Marieta Hristozkova
  • Maria Geneva
  • Ira StanchevaEmail author
  • G. Georgiev


The application of nutrients to the roots and leaves of inoculated pea plants grown under conditions of reduced Mo supply was studied. Pea plants (Pisum sativum L.) were grown on liquid nutrient solution excluding Mo from the media until the 35th day under glasshouse conditions. Plants were inoculated with the bacterial suspension of Rhizobium leguminosarum Bv. Vicae, strain D293 at approximately 108 cells per cm3. The foliar fertilizer Agroleaf® was applied at 0.3% concentration. Changes in the root nodulation and the activities of the enzymes connected with nitrogen assimilation pathway (nitrate reductase - NR-NADH: EC; glutamine synthetase - GS: EC; glutamate synthase - NADH-GOGAT: EC and nitrogenase - NG: EC were observed. It was established that the foliar application of nutrients reduced the inhibitory effect on the root nodulation and nitrogen assimilatory enzyme activities due to the Mo shortage.


pea foliar fertilizer nitrogen assimilation molybdenum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Atkins, C. A., Rainbird, R. M., Pate, J. S. (1980) Evidence for a purine pathway of ureide synthesis in N-2 fixing nodules of cowpea (Vigna unguiculata L. Walp.) and soybean (Glycine max L. Merr). Plant Physiol. 70, 55–60.CrossRefGoogle Scholar
  2. 2.
    Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem. 72, 248–254.CrossRefGoogle Scholar
  3. 3.
    Campbell, W. H. (1999) Nitrate reductase structure, function and regulation. Binding the gap between biochemistry and physiology. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 277–303.CrossRefGoogle Scholar
  4. 4.
    Chen, F. L., Cullimore, J. V. (1988) Two isozymes of NADH-dependent glutamate synthase in root nodules of Phaseolus vulgaris L.: purification, properties and activity changes during nodule development. Plant Physiol. 88, 1411–1417.CrossRefGoogle Scholar
  5. 5.
    Frechilla, S., Gonzalez, E. M., Royuela, M., Minchin, F. R., Aparicio-Tejo, P.M., Arrese-Igor, C. (2000) Source of nitrogen nutrition (nitrogen fixation or nitrate assimilation) is a major factor involved in pea response to moderate water stress. J. Plant Physiol. 157, 609–617.CrossRefGoogle Scholar
  6. 6.
    Hageman, R. H., Reed, A. J. (1980) Nitrate reductase from higher plants. Method Enzymol. 69, 270–280.CrossRefGoogle Scholar
  7. 7.
    Hardy, R. W. F., Burns, R. C., Holsten, R. D. (1973) Applications of the acetylene reduction assay for measurement of nitrogen fixation. Soil Biol. Biochem. 5, 47–81.CrossRefGoogle Scholar
  8. 8.
    Hristozkova, M., Stancheva, I., Geneva, M. (2005) Response of inoculated pea plants (Pisum sati-vum L.) to foliar fertilizer application with elevated concentrations. Ecol. Future 1, 14–17.Google Scholar
  9. 9.
    Ireland, R. J. Lea, P. J. (1999) The enzymes of glutamine, glutamate, asparagines, and aspartate metabolism. In: Singh B. K. (ed.) Plant amino acids. Biochemistry and biotechnology. Marcel Dekker Inc., New York, pp. 49–109.Google Scholar
  10. 10.
    Jongruaysup, S., Dell, B., Bell, R. W., O’Hara, G. W., Bradley, J. S. (1997) Effect of molybdenum and inorganic nitrogen on molybdenum redistribution in black gram (Vigna mungo L. Hepper) with particular reference to seed fill. Ann. Bot. 79, 67–74.CrossRefGoogle Scholar
  11. 11.
    Kaiser, B. N., Gridley, K. L., Brady, J. N., Phillips, T., Tyerman, S. D. (2005) The role of molybdenum in agricultural plant production. Ann. Bot. 96, 745–754.CrossRefGoogle Scholar
  12. 12.
    Lam, H. M. Coshigano, K. T., Oliveira, I. C., Melo-Oliveira, R., Coruzzi, G. M. (1996) The molecular-genetics of nitrogen assimilation into amino acids in higher plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 47, 569–593.CrossRefGoogle Scholar
  13. 13.
    Mallarino, A., UI-Haq, M. (1998) What about foliar fertilization of soybeans? Fluid J. 31, 8–11.Google Scholar
  14. 14.
    Marschner, H. (1995) Mineral nutrition of higher plants. 2nd Edition. Academic Press, London.Google Scholar
  15. 15.
    Mendel, R. R., Haensch, R. (2002) Molybdoenzymes and molybdenum cofactor in plants. Can. J. Bot. 72, 739–750.Google Scholar
  16. 16.
    Miflin, B. J., Lea, P. J. (1982) Amino acid metabolism. Annu. Rev. Plant Physiol. 28, 299–329.CrossRefGoogle Scholar
  17. 17.
    O’Neal, D., Joy, K. W. (1973) Glutamine synthetase of pea leaves, I. Purification, stabilization and pH optima. Arch. Biochem. Biophys. 159, 113–122.CrossRefGoogle Scholar
  18. 18.
    Oaks, A. (1994) Primary nitrogen assimilation in higher plants and its regulation. J. Exp. Bot. 53, 1689–1698.Google Scholar
  19. 19.
    Pate, J. S. (1973) Uptake, assimilation and transport of nitrogen compounds by plants. Soil Biol. Biochem. 5, 109–119.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Marieta Hristozkova
    • 1
  • Maria Geneva
    • 1
  • Ira Stancheva
    • 1
    Email author
  • G. Georgiev
    • 1
  1. 1.Department of Mineral Nutrition and Water Relations, Acad. M. Popov Institute of Plant PhysiologyBulgarian Academy of SciencesSofiaBulgaria

Personalised recommendations