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Acta Biologica Hungarica

, Volume 58, Issue 1, pp 75–86 | Cite as

Studies on the Antibacterial Activities and Mechanisms of Chitosan Obtained from Cuticles of Housefly Larvae

  • Y. J. JingEmail author
  • Y. J. Hao
  • H. Qu
  • Y. Shan
  • D. S. Li
  • R. Q. Du
Article

Abstract

Chitosan was obtained from cuticles of the housefly (Musca domestica) larvae. Antibacterial activities of different Mw chitosans were examined against six bacteria. Antibacterial mechanisms of chitosan were investigated by measuring permeability of bacterial cell membranes and observing integrity of bacterial cells. Results show that the antibacterial activity of chitosan decreased with increase in Mw. Chitosan showed higher antibacterial activity at low pH. Ca2+ and Mg2+ could markedly reduce the antibacterial activity of chitosan. The minimum inhibitory concentrations of chitosans ranged from 0.03% ~ 0.25% and varied with the type of bacteria and Mw of chitosan. Chitosan could cause leakage of cell contents of the bacteria and disrupt the cell wall.

Keywords

molecular weight chitosan antibacterial activity antibacterial mechanism housefly larvae 

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Notes

Acknowledgements

We wish to thank Dr. K. Hou and Y. Wang for critical reading.

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Y. J. Jing
    • 1
    Email author
  • Y. J. Hao
    • 1
  • H. Qu
    • 1
  • Y. Shan
    • 1
  • D. S. Li
    • 1
  • R. Q. Du
    • 1
  1. 1.Department of Genetics, College of Life ScienceNankai UniversityTianjinP. R. China

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