Correlation between First Polar Body Morphology and Further Embryo Development

Abstract

First polar body (PB) morphology of human oocytes can indicate further embryo development and viability. However, controversial data have been published in this topic. Our retrospective study analyses the fertilization and further development of oocytes in relation to different morphological features of the first PB. The morphology of 3387 MII oocytes from 522 in vitro fertilization (IVF) treatments were assessed before intracytoplasmic sperm injection (ICSI). Oocytes were classified according to their first PB morphology. Assessment of fertilization and embryonic development (cell number, embryo grade, amount of anuclear fragmentation and presence of multinucleated blastomeres) was performed 16–20 and 42–48 hours after ICSI. Our results show that fertilization rate and embryo quality is influenced by PB morphology, while speed of development is not affected by the morphology of the first PB. Contrary to previous findings, our results suggest that oocytes with a fragmented PB had a higher developmental ability than those with an intact PB. However, we observed a lower viability of oocytes with a large PB. Since there are contradictions in this and previous observations, an extensive study is needed with standard hormonal stimulation protocol and oocyte evaluation criteria.

References

  1. 1.

    Balaban, B., Urman, B., Sertac, A., Alatas, C., Senai, A., Mercan, R. (1998) Oocyte morphology does not affect fertilization rate, embryo quality and implantation rate after intracytoplasmic sperm injection. Hum. Reprod. 13, 3431–3433.

    CAS  Article  Google Scholar 

  2. 2.

    Borini, A., Lagalla, C., Cattoli, M., Sereni, E., Sciajno, E., Flamigni, C., Coticchio, G. (2005) Predictive factors for embryo implantation potential. Reprod. Biomed. Online 10, 653–668.

    Article  Google Scholar 

  3. 3.

    Ciotti, P. M., Notarangelo, L., Morselli-Labate, A. M., Felletti, V., Porcu, E., Venturoli S. (2004) First polar body morphology before ICSI is not related to embryo quality or pregnancy rate. Hum. Reprod. 19, 2334–2339.

    CAS  Article  Google Scholar 

  4. 4.

    De Shutter, P., Dozortsev, D., Qian, C., Dhont, M. (1996) Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmatic sperm injection. Hum. Reprod. 11, 595–597.

    Article  Google Scholar 

  5. 5.

    Devreker, F., Emiliani, S., Revelard, P., Van den Bergh, M., Govaerts, I., Englert, Y. (1999) Comparison of two elective transfer policies of two embryos to reduce multiple pregnancies without impairing pregnancy rates. Hum. Reprod. 14, 83–89.

    CAS  Article  Google Scholar 

  6. 6.

    Ebner, T., Moser, M., Sommergruber, M., Tews, G. (2003) Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review. Hum. Reprod. Update 9, 251–262.

    CAS  Article  Google Scholar 

  7. 7.

    Ebner, T., Moser, M., Sommergruber, M., Yaman, C., Pfleger, U., Tews, G. (2002) First polar body morphology and blastocyst formation rate in ICSI patients. Hum. Reprod. 17, 2415–2418.

    CAS  Article  Google Scholar 

  8. 8.

    Ebner, T., Moser, M., Yaman, C., Feichtinger, O., Hartl, J., Tews, G. (1999) Elective transfer of embryos selected on the basis of first polar body morphology is associated with increased rates of implantation and pregnancy. Fertil. Steril. 72, 599–603.

    CAS  Article  Google Scholar 

  9. 9.

    Ebner, T., Yaman, C., Moser, M., Sommergruber, M., Feicthinger, O., Tews, G. (2000) Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum. Reprod. 15, 427–430.

    CAS  Article  Google Scholar 

  10. 10.

    Eichenlaub-Ritter, U., Schmiady, H., Kentenich, H., Soewarto, D. (1995) Recurrent failure in polar body formation and premature chromosome condensation in oocytes from a human patient: indicators of asynchrony in nuclear and cytoplasmic maturation. Hum. Reprod. 10, 2343–2349.

    CAS  Article  Google Scholar 

  11. 11.

    Fancsovits, P., Tóth, L., Takács, F. Z., Murber, A., Papp, Z., Urbancsek, J. (2005) Early pronuclear breakdown can be as good indicator of embryo viability as early cleavage. Fertil. Steril. 84, 881–887.

    Article  Google Scholar 

  12. 12.

    Fancsovits, P., Urbancsek, J., Papp, Z. (2002) In vitro fertilisatióval létrehozott praeembryók beágyazódási esélye a petesejt, a zigóta és a praeembryo morphologiai jellemzői alapján. Magyar Nőorv. L. 65, 231–242.

    Google Scholar 

  13. 13.

    Klingman, I., Benadiva, C., Alikani, M., Munné, S. (1996) The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities. Hum. Reprod. 11, 1492–1498.

    Article  Google Scholar 

  14. 14.

    Shoukir, Y., Campana, A., Farley, T., Sakkas, D. (1997) Early cleavage of in-vitro fertilized human embryos to the 2-cell stage: a novel indicator of embryo quality and viability. Hum. Reprod. 12, 1531–1536.

    CAS  Article  Google Scholar 

  15. 15.

    Tesarik, J., Greco, E. (1999) The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Hum. Reprod. 14, 1318–1323.

    CAS  Article  Google Scholar 

  16. 16.

    Van Royen, E., Mangelschots, K., De Neubourg, D., Valkenburg, M., Van de Meerssche, M., Ryckaert, G., Eestermans, W., Gerris, J. (1999) Characterization of a top quality embryo, a step towards single embryo transfer. Hum. Reprod. 14, 2345–2349.

    Article  Google Scholar 

  17. 17.

    Van Royen, E., Mangelschots, K., Vercruyssen, M., De Neuborg, D., Valkenburg, M., Ryckaert, G., Gerris, J. (2003) Multinucleation in cleavage stage embryos. Hum. Reprod. 18, 1062–1069.

    Article  Google Scholar 

  18. 18.

    Veeck, L. L. (1991) Atlas of the human oocyte and early conceptus. Vol II. Williams&Wilkins, Baltimore, pp. 1–255.

    Google Scholar 

  19. 19.

    Verlinsky, Y. (2003) Is there any predictive value of first polar body morphology for embryo genotype or developmental potential? Reprod. Biomed. Online. 7, 336–341.

    Article  Google Scholar 

  20. 20.

    Xia, P. (1997) Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum. Reprod. 12, 1750–1755.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to P. Fancsovits.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Fancsovits, P., Tóthné, Z.G., Murber, Á. et al. Correlation between First Polar Body Morphology and Further Embryo Development. BIOLOGIA FUTURA 57, 331–338 (2006). https://doi.org/10.1556/ABiol.57.2006.3.7

Download citation

Keywords

  • First polar body
  • oocyte quality
  • embryo quality
  • in vitro fertilization