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Acta Biologica Hungarica

, Volume 57, Issue 2, pp 191–200 | Cite as

A Propolis Extract and the Labeling of Blood Constituents with Technetium-99m

  • L. M. Jesus
  • P. R. C. Abreu
  • Marcela C. Almeida
  • Lavínia C. Brito
  • Sheila F. Soares
  • D. E. de Souza
  • Luciana C. Bernardo
  • A. S. FonsecaEmail author
  • M. Bernardo-Filho
Article

Abstract

Since ancient times propolis has been employed for many human purposes because to their favourable properties. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine procedures. Some authors have reported that synthetic or natural drugs can interfere with the labeling of blood constituents with 99mTc. The aim of this work was to evaluate the action of a propolis extract on the labeling of blood elements with 99mTc. Samples of whole blood of male Wistar rats were incubated in sequence with an aqueous propolis extract at different concentrations, stannous chloride and 99mTc, as sodium pertechnetate. Blood samples were centrifuged to separate plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were also separated after precipitation in trichloroacetic acid solution and centrifugation. The radioactivity was counted and the percentage of incorporated radioactivity (%ATI) for each fraction was calculated. The data obtained showed that the aqueous propolis extract used decreased significantly the %ATI in plasma proteins at higher concentration studied. Results suggest that at high concentration the constituents of this extract could alter the labeling of plasma proteins competing with same binding sites of the 99mTc on the plasma proteins or acting as antioxi-dant compounds.

Keywords

Propolis blood constituents plasma proteins technetium-99m 

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© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • L. M. Jesus
    • 1
  • P. R. C. Abreu
    • 1
  • Marcela C. Almeida
    • 1
  • Lavínia C. Brito
    • 1
  • Sheila F. Soares
    • 1
  • D. E. de Souza
    • 1
  • Luciana C. Bernardo
    • 1
  • A. S. Fonseca
    • 2
    Email author
  • M. Bernardo-Filho
    • 1
  1. 1.Departamento de Biofísica e BiometriaInstituto de Biologia Roberto Alcantara GomesRio de JaneiroBrasil
  2. 2.Departamento de Farmacologia e PsicobiologiaInstituto de Biologia Roberto Alcantara GomesRio de JaneiroBrasil

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