Advertisement

Acta Biologica Hungarica

, Volume 57, Issue 1, pp 81–95 | Cite as

Synthesis of Soluble Heat Shock Proteins in Seminal Root Tissues of Some Cultivated and Wild Wheat Genotypes

  • M. YildizEmail author
  • S. Terzoglu
Article

Abstract

Effect of heat stress on the synthesis of soluble heat shock proteins (HSPs) and the regrowth in seminal roots of three cultivated and three wild wheat genotypes was examined. In regrowth experiments, 2-dold etiolated seedlings were exposed to 23 (control), 32, 35, 37 and 38 °C for 24 h, and 35 and 37 °C (24 h) followed by 50 °C (1 h). The lengths of the seminal roots generally decreased significantly at the end of 48 and 72 h recovery growth periods at 35, 37 and 38 °C temperature treatments compared with control. Genotypic variability was significant level at all temperature treatments for the seminal root length. Also, genotypic differences for the number of seminal roots were determined among the wheat cultivars and between the wild wheat species and the wheat cultivars at all temperature treatments; but genotypic differences among wild wheat species were only detected at 37→50 °C treatment. Acquired thermotolerance for the seminal root length is over 50% at 37→50 °C treatment. The genotypic variability of soluble heat shock proteins in seminal root tissues were analyzed by two-dimensional electrophoresis (2-DE). Total number of low molecular weight (LMW) HSPs was more than intermediate- (IMW) and high- (HMW) HSPs at high temperature treatments. The most of LMW HSPs which were generally of acidic character ranged between 14.2–30.7 kDa. The genotypes had both common (43 HSP spots between at least two genotypes and 23 HSP spots between 37 and 37→50 °C) and genotype-specific (72 HSP spots) LMW HSPs.

Keywords

Triticum Aegilops seminal root regrowth heat shock proteins 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgements

This study is a part of PhD thesis and was supported by Scientific and Technical Research Council of Turkey (TUBITAK) Project No. TOGTAG-1679 and Government Planning Organization (DPT) Project No. 97 K 121290.

References

  1. 1.
    Blum, H., Beier, H., Gross, H. J. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93–99.Google Scholar
  2. 2.
    Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.CrossRefGoogle Scholar
  3. 3.
    Burke, J. J. (2001) Identification of genetic diversity and mutations in higher plant acquired thermotolerance. Physiol. Plant. 112, 167–170.CrossRefGoogle Scholar
  4. 4.
    Chen, H.-H., Shen, Z.-Y., Li, P. H. (1982) Adaptability of crop plants to high temperature stress. Crop Sci. 22, 719–725.CrossRefGoogle Scholar
  5. 5.
    Cooper, P., Ho, T.-H. D. (1983) Heat shock proteins in maize. Plant Physiol. 71, 215–222.CrossRefGoogle Scholar
  6. 6.
    Cooper, P., Ho, T.-H. D., Hauptmann, R. M. (1984) Tissue specificity of the heat-shock response in maize. Plant Physiol. 75, 431–441.CrossRefGoogle Scholar
  7. 7.
    Damerval, C., de Vienne, D., Zivy, M., Thiellement, H. (1986) Technical improvements in twodimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7, 52–54.CrossRefGoogle Scholar
  8. 8.
    Hochstrasser, D. F., Harrington, M. G., Hochstrasser, A.-C., Miller, M. J., Merril, C. R. (1988) Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424–435.CrossRefGoogle Scholar
  9. 9.
    Howarth, C. J. (1996) Growth and survival at extreme temperatures: Implications for crop improvement. In: Chopra, V. L., Singh, R. B., Varma, A. (eds) Crop Productivity and Sustainability Shaping the Future. Oxford & IBH Publishing, New Delhi and Calcutta, pp. 467–483.Google Scholar
  10. 10.
    Huang, B.-R., Taylor, H. M., McMichael, B. L. (1991) Growth and development of seminal and crown roots of wheat seedlings as affected by temperature. Envir. Exp. Bot. 31, 471–477.CrossRefGoogle Scholar
  11. 11.
    Huang, B.-R., Taylor, H. M., McMichael, B. L. (1991) Behavior of lateral roots in winter wheat as affected by temperature. Envir. Exp. Bot. 31, 187–192.CrossRefGoogle Scholar
  12. 12.
    Key, J. L., Kimpel, J. A., Lin, C. Y., Nagao, R. T., Vierling, E., Czarnecka, E., Gurley, W. B., Roberts, J. K., Mansfield, M. A., Edelman, L. (1985) The heat shock response in soybean. In: Key, J. L., Kosuge, T. (eds) Cellular and Molecular Biology of Plant Stress. Alan R Liss, New York, pp. 161–179.Google Scholar
  13. 13.
    Kimpel, J. A., Key, J. L. (1985) Heat shock in plants. Trends Biochem. Sci. 11, 353–357.CrossRefGoogle Scholar
  14. 14.
    Krishnan, M., Nguyen, H. T., Burke, J. J. (1989) Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol. 90, 140–145.CrossRefGoogle Scholar
  15. 15.
    Krishnan, M., Nguyen, H. T. (1990) Drying acrylamide slab gels for fluorography without using gel drier and vacuum pump. Anal. Biochem. 187, 51–53.CrossRefGoogle Scholar
  16. 16.
    Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle Scholar
  17. 17.
    Lin, C.-Y., Roberts, J. K., Key, J. L. (1984) Acquisition of thermotolerance in soybean seedlings. Synthesis and accumulation of heat shock proteins and their cellular localization. Plant Physiol. 35, 152–160.CrossRefGoogle Scholar
  18. 18.
    Mansfield, M. A., Key, J. L. (1987) Synthesis of the low molecular weight heat shock proteins in plants. Plant Physiol. 84, 1007–1017.CrossRefGoogle Scholar
  19. 19.
    Mishra, S. K., Tripp, J., Winkelhaus, S., Tschiersch, B., Theres, K., Nover, L., Scharf, K. D. (2002) In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Gene Dev. 16, 1555–1567.CrossRefGoogle Scholar
  20. 20.
    Naqvi, S. M. S., Özalp, V. C., Öktem, H. A., Yücel, M. (1994) Two-dimensional electrophoresis of proteins with a different approach to isoelectric focusing. Analyst 119, 1341–1344.CrossRefGoogle Scholar
  21. 21.
    Necchi, A., Pogna, N. E., Mapelli, S. (1987) Early and late heat shock proteins in wheats and other cereal species. Plant Physiol. 84, 1378–1384.CrossRefGoogle Scholar
  22. 22.
    Nguyen, H. T., Hendershot, K. L., Joshi, C. P. (1992) Molecular genetics for stress breeding: heatshock proteins. In: Buxton, D. R. et al. (eds) Crop Science Society of America, Maryland. Int. Crop Sci. 1, 541–547.Google Scholar
  23. 23.
    O’Farrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. The J. of Biol. Chem. 250, 4007–4021.Google Scholar
  24. 24.
    Passioura, J. B. (1974) The effect of root geometry on the water relations of temperate cereals (wheat, barley, oats). In: Kelek, J. (ed.) Structure and Function of Primary Root Tissue. Veda Publishing, Bratislava, pp. 357–363.Google Scholar
  25. 25.
    Ramagli, L. S., Rodriguez, L. V. (1985) Quantitation of microgram amounts of protein in two-dimensional polyacrylamide gel electrophoresis sample buffer. Electrophoresis 6, 559–563.CrossRefGoogle Scholar
  26. 26.
    Srikanthbabu, V., Kumar, G., Krishnaprasad, B. T., Gopalakrishna, R., Savitha, M., Udayakumar, M. (2002) Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR). J. Plant Physiol. 159, 535–545.CrossRefGoogle Scholar
  27. 27.
    Stone, P. J., Nicolas, M. E. (1994) Wheat cultivars vary widely in their responses of grain yield and quality to short period of post anthesis heat stress. Aust. J. Plant Physiol. 21, 887–900.Google Scholar
  28. 28.
    Sun, W., Montagu, M. V., Verbruggen, N. (2002) Small heat shock proteins and stress tolerance in plants. Biochimica et Biophysica Acta 1577, 1–9.CrossRefGoogle Scholar
  29. 29.
    Vierling, R. A., Nguyen, H. T. (1990) Heat shock protein synthesis and accumulation in diploid wheat. Crop Sci. 30, 1337–1342.CrossRefGoogle Scholar
  30. 30.
    Vierling, E. (1991) The roles of heat shock proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 579–620.CrossRefGoogle Scholar
  31. 31.
    Waters, E. R., Lee, G. J., Vierling, E. (1996) Evolution, structure and function of the small heat shock proteins in plants. J. Exper. Bot. 47, 325–338.CrossRefGoogle Scholar
  32. 32.
    Wrigley, C. W., Blumenthal, C., Gras, P. W., Barlow, E. W. R. (1994) Temperature variation during grain filling and changes in wheat-grain quality. Australian J. of Plant Physiol. 21, 875–885.Google Scholar
  33. 33.
    Wu, M.-T., Wallner, S. J. (1983) Heat stress responses in cultured plant cells. Development and comparison of viability tests. Plant Physiol. 72, 817–820.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science and ArtsAfyon Kocatepe UniversityAfyonkarahisarTurkey
  2. 2.Department of Biology, Faculty of ScienceHacettepe UniversityAnkaraTurkey

Personalised recommendations