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Acta Biologica Hungarica

, Volume 56, Issue 3–4, pp 233–245 | Cite as

Detection of Nanobacteria-Like Particles in Human Atherosclerotic Plaques

  • L. G. PuskásEmail author
  • L. Tiszlavicz
  • Zs. Rázga
  • L. L. Torday
  • T. Krenács
  • J. Gy. Papp
Article

Abstract

Recent and historical evidence is consistent with the view that atherosclerosis is an infectious disease or microbial toxicosis impacted by genetics and behavior. Because small bacterial-like particles, also known as nanobacteria have been detected in kidney stones, kidney and liver cyst fluids, and can form a calcium apatite coat we posited that this agent is present in calcified human atherosclerotic plaques. Carotid and aortic atherosclerotic plaques and blood samples collected at autopsy were examined for nanobacteria- like structures by light microscopy (hematoxylin-eosin and a calcium-specific von Kossa staining), immuno-gold labeling for transmission electron microscopy (TEM) for specific nanobacterial antigens, and propagation from homogenized, filtered specimens in culture medium. Nanobacterial antigens were identified in situ by immuno-TEM in 9 of 14 plaque specimens, but none of the normal carotid or aortic tissue (5 specimens). Nanobacteria-like particles were propagated from 26 of 42 sclerotic aorta and carotid samples and were confirmed by dot immunoblot, light microscopy and TEM. [3H]L-aspartic acid was incorporated into high molecular weight compounds of demineralized particles. PCR amplification of 16S rDNA sequences from the particles was unsuccessful by traditional protocols. Identification of nanobacteria-like particles at the lesion supports, but does not by itself prove the hypothesis that these agents contribute to the pathogenesis of atherosclerosis, especially vascular calcifications.

Keywords

Nanobacteria-like particles atherosclerosis carotid artery aorta vascular plaque 

Abbreviations

DMEM

Dulbecco’s Eagle’s medium

TEM

transmission electron microscopy

NB

nanobacteria

EM

electronmicroscopy

FBS

fetal bovine serum

LDL

low density lipoprotein

EDTA

ethylene-diamino-tetraacetate

BSA

bovine serum albumin

PBS

phosphate-buffered saline

FITC

fluorescein-isothiocyanate

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© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • L. G. Puskás
    • 1
    Email author
  • L. Tiszlavicz
    • 3
  • Zs. Rázga
    • 3
  • L. L. Torday
    • 4
  • T. Krenács
    • 3
  • J. Gy. Papp
    • 2
    • 4
  1. 1.Laboratory of Functional GenomicsBiological Research CenterSzegedHungary
  2. 2.Research Unit for Cardiovascular Pharmacology of the Hungarian Academy of SciencesSzegedHungary
  3. 3.Department of PathologyUniversity of SzegedSzegedHungary
  4. 4.Department of Pharmacology and PharmacotherapyUniversity of SzegedSzegedHungary

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