Influence of extremely low frequency electromagnetic field (ELF-EMF) on thyroid gland mast cells was investigated on male Mill Hill rats. Animals were exposed to EMF (50 Hz, 50 μT to 500 μT, 10 V/m) from 24 hours after birth, 7 hours/day, 5 days/week for three months when a part of animals (group I) was sacrificed, while the rest of them were subjected to recovery evaluation and sacrificed after one (group II), two (group III) and three (group IV) weeks following the exposure. Stereological analysis on toluidine blue-stained paraffin sections showed increased volume density of degranulated mast cells in all groups and, except in group III, and numerical density as well, implicating the sensitivity of thyroidal mast cells to power frequency EMFs. Since in our previous investigations, morphofunctional alterations of thyroid gland in rats exposed to ELF-EMF were found the contribution of released mast cell mediators to these changes could be presumed.
Bani-Sacchi, T. (1966) Sul numero e la distribuzione delle mastzellen nell’omento del rato albino in rapporto all’eta ed al sesso. Arch. It. Anat. Embriol. 71, 91–98.
Banovac, K., De Forteza, R. (1992) The effect of mast cell chymase on extracellular matrix: studies in autoimmune thyroiditis and in cultured thyroid cells. Int. Arch. Allergy Immunol. 99, 141–149.
Cook, L. L., Persinger, M. A., Koren, S. A. (2000) Differential effects of low frequency, low intensity (<6 mG) nocturnal magnetic fields upon infiltration of mononuclear cells and numbers of mast cells in Lewis rat brains. Toxicol. Lett. 118, 9–19.
Dominici, M. (1902) Sur une methode de technique histologique appropriee a l’etude du systeme hematopoietique. Compt. Rend. Soc. de Biol. (Paris) 54, 221–223.
Ericson, L. E., Hakarson, R., Melander, A., Owman, C. H. (1972) TSH-induced release of 5-hydrox-ytryptamine and histamine from rat mast cells. Endocrinology 90, 795–801.
House, V. R., McCormick, L. D. (2000) Modulation of natural killer cell function after exposure to 60 Hz magnetic fields: confirmation of the effect in mature B6C3F1 mice. Radiat. Res. 153, 722–724.
Iurina, N. A., Radostina, A. I., Savin, B. M., Remizova, V. A., Kosova, I. P. (1997) Mast cells as a test of body state during electromagnetic exposure of varying intensity. Aviokosm. Ekolog. Med. 31, 43–47.
Ivancsits, S., Diem, E., Pilger, A., Rudiger, H. W., Jahn, O. (2002) Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibrob-lasts. Mutat. Res. 519, 1–13.
Johansson, O., Gangi, S., Liang, Y., Yoshimura, K., Jing, C., Liu, P.-Y. (2001) Cutaneous mast cells are altered in normal healthy volunteers sitting in front of ordinary TVs/PCs-results from open-field provocation experiments. J. Cutan. Pathol. 28, 513–519.
Kalisnik, M. (1981) Morphometry of the thyroid gland. Stereol. Yugosl. 3 (Suppl 1), 547–569.
Lafreniere, G. F., Persinger, M. A. (1979) Thyroid morphology and activity does not respond to ELF electromagnetic field exposures. Experientia 35, 561–562.
Matavulj, M., Rajkovic, V., Kovacevic, T., Sutija, M., Lazetic, B., Uscebrka, G., Stevanovic, D. (1999) Analysis of the effects of a 50 Hz electromagnetic field on number and volume of rat intrathy-roidal mast cells. Proceedings of the International Conference on Stereology, Spatial Statistics and Stochastic Geometry, Prague, pp. 323–328.
Matavulj, M., Rajkovic, V., Uscebrka, G., Zikic, D., Stevanovic, D., Lazetic, B. (1999) Electromagnetic field effects on the morphology of rat thyroid gland. In: Bersani, F. (ed.) Electricity and Magnetism in Biology and Medicine. Kluwer Academic/Plenum Publishers, New York, pp. 489–492.
Melander, A., Owman, C. H., Sundler, F. (1971) TSH-induced appearance and stimulation of amine-containing mast cells in the mouse thyroid. Endocrinology 89, 528–533.
Melander, A., Sundler, F. (1972) Significance of thyroid mast cells in thyroid hormone secretion. Endocrinology 90, 802–807.
Melander, A., Westgren, U., Sundler, F., Ericson, L. E. (1975) Influence of histamine- and 5-hydrox-ytryptamine-containing thyroid mast cells on thyroid blood flow and permeability in the rat. Endocrinology 97, 1130–1137.
Panagopoulos, D. J., Karabarbounis, A., Margaritis, L. H. (2002) Mechanism for action of electromagnetic fields on cells. Biochem. Biophys. Res. Commun. 298, 95–102.
Price, J. A., Strattan, R. D. (1998) Analysis of the effect of a 60 Hz AC field on histamine release by rat peritoneal mast cells. Bioelectromagnetics 19, 192–198.
Quinlan, W. J., Petrondas, D., Lebda, N., Pettit, S., Michaelson, S. M. (1985) Neuroendocrine parameters in the rat exposed to 60 Hz electric fields. Bioelectromagnetics 6, 381–389.
Rajkovic, V., Matavulj, M., Gledic, D., Lazetic, B. (2003) Evaluation of rat thyroid gland morpho-physiological status after three months exposure to 50 Hz electromagnetic field. Tissue & Cell 35, 223–231.
Selmaoui, B., Lambrozo, J., Touitou, Y. (1997) Endocrine functions in young men exposed for one night to a 50 Hz magnetic field. A circadian study of pituitaty, thyroid and adrenocortical hormones. Life Sci. 61, 473–486.
Toda, S., Tokuda, Y., Koike, N., Yonemitsu, N., Watanabe, K., Koike, K., Fujitani, N., Hiromatsu, Y., Sugihara, H. (2000) Growth factor-expressing mast cells accumulate at the thyroid tissue-regenerative site of subacute thyroiditis. Thyroid 10, 381–386.
Udintsev, N. A., Serebrov, V. Y., Tsyrov, G. I. (1978) Effect of an industrial frequency alternating magnetic field on the functional state of the thyroid gland and thyroxine absorption by the organs of rat. Bull. Eksper. Biologii i Meditsini 86, 544–546. (In Serbian.)
Van Den Heuvel, R., Leppens, H., Nemethova, G., Verschaeve, L. (2001) Haemopoietic cell proliferation in murine bone marrow cells exposed to extreme low frequency (ELF) electromagnetic fields. Toxicol. In Vitro 15, 351–355.
Wynford-Thomas, D., Stringer, B. M. (1982) Mast cell hyperplasia during goitrogen-induced thyroid growth - a quantitative study. Acta Endocrinol. (Copenh) 101, 365–370.
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Rajkovic, V., Matavulj, M. & Lazetic, B. Stereological Analysis of Thyroid Mast Cells in Rats After Exposure to Extremely Low Frequency Electromagnetic Field and the Following “off” Field Period. BIOLOGIA FUTURA 56, 43–51 (2005). https://doi.org/10.1556/ABiol.56.2005.1-2.5
- Electromagnetic field
- mast cell
- thyroid gland