Stereological Analysis of Thyroid Mast Cells in Rats After Exposure to Extremely Low Frequency Electromagnetic Field and the Following “off” Field Period

Abstract

Influence of extremely low frequency electromagnetic field (ELF-EMF) on thyroid gland mast cells was investigated on male Mill Hill rats. Animals were exposed to EMF (50 Hz, 50 μT to 500 μT, 10 V/m) from 24 hours after birth, 7 hours/day, 5 days/week for three months when a part of animals (group I) was sacrificed, while the rest of them were subjected to recovery evaluation and sacrificed after one (group II), two (group III) and three (group IV) weeks following the exposure. Stereological analysis on toluidine blue-stained paraffin sections showed increased volume density of degranulated mast cells in all groups and, except in group III, and numerical density as well, implicating the sensitivity of thyroidal mast cells to power frequency EMFs. Since in our previous investigations, morphofunctional alterations of thyroid gland in rats exposed to ELF-EMF were found the contribution of released mast cell mediators to these changes could be presumed.

References

  1. 1.

    Bani-Sacchi, T. (1966) Sul numero e la distribuzione delle mastzellen nell’omento del rato albino in rapporto all’eta ed al sesso. Arch. It. Anat. Embriol. 71, 91–98.

    Google Scholar 

  2. 2.

    Banovac, K., De Forteza, R. (1992) The effect of mast cell chymase on extracellular matrix: studies in autoimmune thyroiditis and in cultured thyroid cells. Int. Arch. Allergy Immunol. 99, 141–149.

    CAS  Article  Google Scholar 

  3. 3.

    Cook, L. L., Persinger, M. A., Koren, S. A. (2000) Differential effects of low frequency, low intensity (<6 mG) nocturnal magnetic fields upon infiltration of mononuclear cells and numbers of mast cells in Lewis rat brains. Toxicol. Lett. 118, 9–19.

    CAS  Article  Google Scholar 

  4. 4.

    Dominici, M. (1902) Sur une methode de technique histologique appropriee a l’etude du systeme hematopoietique. Compt. Rend. Soc. de Biol. (Paris) 54, 221–223.

    Google Scholar 

  5. 5.

    Ericson, L. E., Hakarson, R., Melander, A., Owman, C. H. (1972) TSH-induced release of 5-hydrox-ytryptamine and histamine from rat mast cells. Endocrinology 90, 795–801.

    CAS  Article  Google Scholar 

  6. 6.

    House, V. R., McCormick, L. D. (2000) Modulation of natural killer cell function after exposure to 60 Hz magnetic fields: confirmation of the effect in mature B6C3F1 mice. Radiat. Res. 153, 722–724.

    CAS  Article  Google Scholar 

  7. 7.

    Iurina, N. A., Radostina, A. I., Savin, B. M., Remizova, V. A., Kosova, I. P. (1997) Mast cells as a test of body state during electromagnetic exposure of varying intensity. Aviokosm. Ekolog. Med. 31, 43–47.

    CAS  Google Scholar 

  8. 8.

    Ivancsits, S., Diem, E., Pilger, A., Rudiger, H. W., Jahn, O. (2002) Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibrob-lasts. Mutat. Res. 519, 1–13.

    CAS  Article  Google Scholar 

  9. 9.

    Johansson, O., Gangi, S., Liang, Y., Yoshimura, K., Jing, C., Liu, P.-Y. (2001) Cutaneous mast cells are altered in normal healthy volunteers sitting in front of ordinary TVs/PCs-results from open-field provocation experiments. J. Cutan. Pathol. 28, 513–519.

    CAS  Article  Google Scholar 

  10. 10.

    Kalisnik, M. (1981) Morphometry of the thyroid gland. Stereol. Yugosl. 3 (Suppl 1), 547–569.

    Google Scholar 

  11. 11.

    Lafreniere, G. F., Persinger, M. A. (1979) Thyroid morphology and activity does not respond to ELF electromagnetic field exposures. Experientia 35, 561–562.

    CAS  Article  Google Scholar 

  12. 12.

    Matavulj, M., Rajkovic, V., Kovacevic, T., Sutija, M., Lazetic, B., Uscebrka, G., Stevanovic, D. (1999) Analysis of the effects of a 50 Hz electromagnetic field on number and volume of rat intrathy-roidal mast cells. Proceedings of the International Conference on Stereology, Spatial Statistics and Stochastic Geometry, Prague, pp. 323–328.

    Google Scholar 

  13. 13.

    Matavulj, M., Rajkovic, V., Uscebrka, G., Zikic, D., Stevanovic, D., Lazetic, B. (1999) Electromagnetic field effects on the morphology of rat thyroid gland. In: Bersani, F. (ed.) Electricity and Magnetism in Biology and Medicine. Kluwer Academic/Plenum Publishers, New York, pp. 489–492.

    Google Scholar 

  14. 14.

    Melander, A., Owman, C. H., Sundler, F. (1971) TSH-induced appearance and stimulation of amine-containing mast cells in the mouse thyroid. Endocrinology 89, 528–533.

    CAS  Article  Google Scholar 

  15. 15.

    Melander, A., Sundler, F. (1972) Significance of thyroid mast cells in thyroid hormone secretion. Endocrinology 90, 802–807.

    CAS  Article  Google Scholar 

  16. 16.

    Melander, A., Westgren, U., Sundler, F., Ericson, L. E. (1975) Influence of histamine- and 5-hydrox-ytryptamine-containing thyroid mast cells on thyroid blood flow and permeability in the rat. Endocrinology 97, 1130–1137.

    CAS  Article  Google Scholar 

  17. 17.

    Panagopoulos, D. J., Karabarbounis, A., Margaritis, L. H. (2002) Mechanism for action of electromagnetic fields on cells. Biochem. Biophys. Res. Commun. 298, 95–102.

    CAS  Article  Google Scholar 

  18. 18.

    Price, J. A., Strattan, R. D. (1998) Analysis of the effect of a 60 Hz AC field on histamine release by rat peritoneal mast cells. Bioelectromagnetics 19, 192–198.

    CAS  Article  Google Scholar 

  19. 19.

    Quinlan, W. J., Petrondas, D., Lebda, N., Pettit, S., Michaelson, S. M. (1985) Neuroendocrine parameters in the rat exposed to 60 Hz electric fields. Bioelectromagnetics 6, 381–389.

    CAS  Article  Google Scholar 

  20. 20.

    Rajkovic, V., Matavulj, M., Gledic, D., Lazetic, B. (2003) Evaluation of rat thyroid gland morpho-physiological status after three months exposure to 50 Hz electromagnetic field. Tissue & Cell 35, 223–231.

    CAS  Article  Google Scholar 

  21. 21.

    Selmaoui, B., Lambrozo, J., Touitou, Y. (1997) Endocrine functions in young men exposed for one night to a 50 Hz magnetic field. A circadian study of pituitaty, thyroid and adrenocortical hormones. Life Sci. 61, 473–486.

    CAS  Article  Google Scholar 

  22. 22.

    Toda, S., Tokuda, Y., Koike, N., Yonemitsu, N., Watanabe, K., Koike, K., Fujitani, N., Hiromatsu, Y., Sugihara, H. (2000) Growth factor-expressing mast cells accumulate at the thyroid tissue-regenerative site of subacute thyroiditis. Thyroid 10, 381–386.

    CAS  Article  Google Scholar 

  23. 23.

    Udintsev, N. A., Serebrov, V. Y., Tsyrov, G. I. (1978) Effect of an industrial frequency alternating magnetic field on the functional state of the thyroid gland and thyroxine absorption by the organs of rat. Bull. Eksper. Biologii i Meditsini 86, 544–546. (In Serbian.)

    CAS  Google Scholar 

  24. 24.

    Van Den Heuvel, R., Leppens, H., Nemethova, G., Verschaeve, L. (2001) Haemopoietic cell proliferation in murine bone marrow cells exposed to extreme low frequency (ELF) electromagnetic fields. Toxicol. In Vitro 15, 351–355.

    Article  Google Scholar 

  25. 25.

    Wynford-Thomas, D., Stringer, B. M. (1982) Mast cell hyperplasia during goitrogen-induced thyroid growth - a quantitative study. Acta Endocrinol. (Copenh) 101, 365–370.

    CAS  Article  Google Scholar 

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Correspondence to Vesna Rajkovic.

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Rajkovic, V., Matavulj, M. & Lazetic, B. Stereological Analysis of Thyroid Mast Cells in Rats After Exposure to Extremely Low Frequency Electromagnetic Field and the Following “off” Field Period. BIOLOGIA FUTURA 56, 43–51 (2005). https://doi.org/10.1556/ABiol.56.2005.1-2.5

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Keywords

  • Electromagnetic field
  • mast cell
  • thyroid gland
  • rat
  • stereology