Stereological Analysis of Thyroid Mast Cells in Rats After Exposure to Extremely Low Frequency Electromagnetic Field and the Following “off” Field Period
Influence of extremely low frequency electromagnetic field (ELF-EMF) on thyroid gland mast cells was investigated on male Mill Hill rats. Animals were exposed to EMF (50 Hz, 50 μT to 500 μT, 10 V/m) from 24 hours after birth, 7 hours/day, 5 days/week for three months when a part of animals (group I) was sacrificed, while the rest of them were subjected to recovery evaluation and sacrificed after one (group II), two (group III) and three (group IV) weeks following the exposure. Stereological analysis on toluidine blue-stained paraffin sections showed increased volume density of degranulated mast cells in all groups and, except in group III, and numerical density as well, implicating the sensitivity of thyroidal mast cells to power frequency EMFs. Since in our previous investigations, morphofunctional alterations of thyroid gland in rats exposed to ELF-EMF were found the contribution of released mast cell mediators to these changes could be presumed.
KeywordsElectromagnetic field mast cell thyroid gland rat stereology
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- 1.Bani-Sacchi, T. (1966) Sul numero e la distribuzione delle mastzellen nell’omento del rato albino in rapporto all’eta ed al sesso. Arch. It. Anat. Embriol. 71, 91–98.Google Scholar
- 4.Dominici, M. (1902) Sur une methode de technique histologique appropriee a l’etude du systeme hematopoietique. Compt. Rend. Soc. de Biol. (Paris) 54, 221–223.Google Scholar
- 7.Iurina, N. A., Radostina, A. I., Savin, B. M., Remizova, V. A., Kosova, I. P. (1997) Mast cells as a test of body state during electromagnetic exposure of varying intensity. Aviokosm. Ekolog. Med. 31, 43–47.Google Scholar
- 10.Kalisnik, M. (1981) Morphometry of the thyroid gland. Stereol. Yugosl. 3 (Suppl 1), 547–569.Google Scholar
- 12.Matavulj, M., Rajkovic, V., Kovacevic, T., Sutija, M., Lazetic, B., Uscebrka, G., Stevanovic, D. (1999) Analysis of the effects of a 50 Hz electromagnetic field on number and volume of rat intrathy-roidal mast cells. Proceedings of the International Conference on Stereology, Spatial Statistics and Stochastic Geometry, Prague, pp. 323–328.Google Scholar
- 13.Matavulj, M., Rajkovic, V., Uscebrka, G., Zikic, D., Stevanovic, D., Lazetic, B. (1999) Electromagnetic field effects on the morphology of rat thyroid gland. In: Bersani, F. (ed.) Electricity and Magnetism in Biology and Medicine. Kluwer Academic/Plenum Publishers, New York, pp. 489–492.CrossRefGoogle Scholar
- 23.Udintsev, N. A., Serebrov, V. Y., Tsyrov, G. I. (1978) Effect of an industrial frequency alternating magnetic field on the functional state of the thyroid gland and thyroxine absorption by the organs of rat. Bull. Eksper. Biologii i Meditsini 86, 544–546. (In Serbian.)Google Scholar
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