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Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 253–262 | Cite as

Mitochondrial DNA4977 Deletion in Brain of Newborns Died After Intensive Care

  • Edit A. Nádasi
  • B. Melegh
  • L. Seress
  • G. KosztolányiEmail author
Article

Abstract

Mitochondrial DNA (mtDNA) deletion affecting 4977 base pairs (mtDNA4977), the most common mtDNA mutation in humans, was analysed in brain specimens (frontal, temporal, and cerebellar cortices, caudate nucleus, thalamus, and hippocampus) and in other tissues (blood clot, liver, kidney, heart, and muscle) taken at autopsy of deceased neonates. mtDNA4977 deletion determined by polymerase chain reaction (PCR) could be demonstrated in each neonatal sample, however, quantity of mtDNA4977 deletion was less in the newborn samples than in those of the elderlies. Results obtained suggest that contrary to certain data mtDNA4977 deletion can be present in neonates. The mtDNA4977 deletion could be generated by perinatal hypoxia or temporary oxygen oversaturations during the intensive care of the neonates, as the mtDNA is sensitive to oxidative damage. In combination with other factors an additional causative role of mtDNA4977 deletion reported here cannot be ruled out in development of cerebral palsy or mental retardation of unknown origin often seen in neonates underwent neonatal intensive care procedures.

Keywords

Mitochondrial DNA deletion ageing brain damage perinatal hypoxia intensive care 

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Notes

Acknowledgement

The work was supported by OTKA grant No. 32556.

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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Edit A. Nádasi
    • 1
  • B. Melegh
    • 1
  • L. Seress
    • 2
  • G. Kosztolányi
    • 1
    • 3
    Email author
  1. 1.Department of Medical Genetics and Child DevelopmentMedical CenterPécsHungary
  2. 2.Central Electronmicroscopic Laboratory, Faculty of MedicineUniversity of PécsPécsHungary
  3. 3.MTA-PTE Clinical Genetics Research GroupPécsHungary

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