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Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 241–252 | Cite as

Subcellular Localization of Components of the Dystrophin Glycoprotein Complex in Cultured Retinal Muller Glial Cells

  • E. Méhes
  • Dominique Mornet
  • Veronika JancsikEmail author
Article

Abstract

The dystrophin glycoprotein complex (DGC) is a membrane-associated protein complex binding extra-cellular matrix (ECM) molecules, such as laminin and forming a bridge towards the cytoskeleton. The molecular composition of the DGC is cell type dependent and it is involved in cell adhesion and motility. Here we present immunocytochemical localization of beta-dystroglycan, the central member of the DGC, utrophin and Dp71f the spliced 71 kDa dystrophin protein product of the DMD gene, in cultured retinal Muller glial cells. It is shown that beta-dystroglycan and utrophin are colocalized in clusters in all parts of Muller cells including the lamellipodium and leading edge of migrating cells. As a contrast, Dp71f labels are distinct from beta-dystroglycan and confined to the perinuclear cytoplasm of Muller cells indicating that Dp71f is not a member of the DGC in cultured Muller cells.

Keywords

Immunocytochemistry rat dystrophin utrophin cytoskeleton 

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Notes

Acknowledgement

This work was supported by the Hungarian Scientific Research Fund (OTKA, T 037597) for V. J.

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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Anatomy and Histology, Faculty of Veterinary ScienceSzent István UniversityBudapestHungary
  2. 2.INSERM U. 128MontpellierFrance

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