Growth and Developmental Responses of Potato to Osmotic Stress Under in vitro Conditions

Abstract

The effect of mannitol on different genotypes of potato was studied in callus and plantlet culture. In vitro responses of five potato genotypes with well-known field behaviour to water deficit were analysed. After a 4-week-long cultivation on media containing mannitol up to 0.8 M, different morpho-physiological parameters were determined and statistically analysed. The useful concentration of mannitol for in vitro screening the osmotic tolerance of different genotypes depended on the type of culture; it was 0.4 M in plantlet-test and 0.8 M in callus-test. In callus-test the relative increase of callus mass was a useful parameter for determination of osmotic tolerance of genotypes at cellular level. In plantlet culture, stress index calculated from the rate of surviving in vitro shoots, number and length of roots per surviving explant and the rate of rooted explants were applicable to determine three groups according to the tolerant, medium tolerant and sensitive categories in agreement with the field behaviour of these genotypes. Under in vitro stress conditions we were able to distinguish the examined genotypes with different drought tolerance.

References

  1. 1.

    Evers, D., Hemmer, K., Hausman, J. F. (1998) Salt stress induced biometric and physiological changes in Solarium tuberosum L. cv. Bintje grown in vitro. Acta Physiol. Plant. 20, 3–7.

    CAS  Article  Google Scholar 

  2. 2.

    Gadallah, M. A. A. (1996) Abscisic acid, temperature and salinity interaction on growth and some mineral elements in Carthamus plants. Plant Growth Regul. 20, 225–236.

    CAS  Article  Google Scholar 

  3. 3.

    Gluska, A. (1999) Root system measurement in assessement of potato cultivar resistance to drought. In: Abstracts of 14thTriennial Conference of EAPR, Sorrento, Italy, 186–187.

    Google Scholar 

  4. 4.

    Harris, P. M. (1978) Water. In: Harris, P. M. (ed.) 772e Potato Crop. Chapman & Hall, London, 244–277.

    Google Scholar 

  5. 5.

    Levitt, J. (1980) Responses of Plants to Environmental Stresses. Vol. 1. Academie Press, New York.

    Google Scholar 

  6. 6.

    Lichtenthaler, H. K. (1996) Vegetation stress: an introduction to the stress concept in plants. J. Plant Physiol. 148, 4–14.

    CAS  Article  Google Scholar 

  7. 7.

    Morgan, J. M. (1984) Osmoregulation and water stress in higher plants. Ann. Rev. Plant Physiol. 35, 299–319.

    Article  Google Scholar 

  8. 8.

    Mullet, J. E., Whitsitt, M. S. (1996) Plant cellular responses to water deficit. Plant Growth Regulation 20, 119–124.

    CAS  Article  Google Scholar 

  9. 9.

    Murashige, T., Skoog, F. (1962) A. revised medium for rapid growth and bioassays with tobacco tissue cultures. Phys. Plant. 15, 473–197.

    CAS  Article  Google Scholar 

  10. 10.

    Paoletti, C., Bagatta, M., DiCandilo, M., Bassi, F., Ranalli, P. (1999) The morpho-physiological response to prolonged water-stress in potato: variation among clones. In: Abstracts of 14thTriennial Conference ofEAPR, Sorrento, Italy, 182–183.

    Google Scholar 

  11. 11.

    Rudorf W. (1958) Dürreresistenz. In: Kappert, H., Rudorf W. (eds) Handbuch der Pflanzen-züchtung. Band III. Züchtung der Knollen- und Wurzelfruchtarten. Paul Parey, Berlin-Hamburg, 134–135.

    Google Scholar 

  12. 12.

    Winter, S. R., Musich, J. T., Portes, K. B. (1988) Evaluation of screening techniques for breeding drought resistant winter wheat. Crop Sci. 28, 512–516.

    Article  Google Scholar 

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Correspondence to Judit Dobránszki.

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Dobránszki, J., Magyar-Tábori, K. & Takács-Hudák, Á. Growth and Developmental Responses of Potato to Osmotic Stress Under in vitro Conditions. BIOLOGIA FUTURA 54, 365–372 (2003). https://doi.org/10.1556/ABiol.54.2003.3-4.14

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Keywords

  • Osmotic stress
  • in vitro
  • genotype-dependence
  • stress index
  • potato