Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 365–372 | Cite as

Growth and Developmental Responses of Potato to Osmotic Stress Under in vitro Conditions

  • Judit DobránszkiEmail author
  • Katalin Magyar-Tábori
  • Ágnes Takács-Hudák


The effect of mannitol on different genotypes of potato was studied in callus and plantlet culture. In vitro responses of five potato genotypes with well-known field behaviour to water deficit were analysed. After a 4-week-long cultivation on media containing mannitol up to 0.8 M, different morpho-physiological parameters were determined and statistically analysed. The useful concentration of mannitol for in vitro screening the osmotic tolerance of different genotypes depended on the type of culture; it was 0.4 M in plantlet-test and 0.8 M in callus-test. In callus-test the relative increase of callus mass was a useful parameter for determination of osmotic tolerance of genotypes at cellular level. In plantlet culture, stress index calculated from the rate of surviving in vitro shoots, number and length of roots per surviving explant and the rate of rooted explants were applicable to determine three groups according to the tolerant, medium tolerant and sensitive categories in agreement with the field behaviour of these genotypes. Under in vitro stress conditions we were able to distinguish the examined genotypes with different drought tolerance.


Osmotic stress in vitro genotype-dependence stress index potato 


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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Judit Dobránszki
    • 1
    Email author
  • Katalin Magyar-Tábori
    • 1
  • Ágnes Takács-Hudák
    • 1
  1. 1.Research Centre of University of DebrecenNyíregyházaHungary

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