Advertisement

Acta Biologica Hungarica

, Volume 53, Issue 3, pp 317–324 | Cite as

Intra- and Interspecific Molecular Polymorphism of Thrips Species

  • K. BayarEmail author
  • O. Törjék
  • Erzsébet Kiss
  • G. Gyulai
  • L. Heszky
Article

Abstract

Molecular polymorphism of six species of Thysanoptera of both sexes, collected from different locations and host plants in Hungary was studied by using RAPD-PCR technique. The specimens were classified according to sampling sites (Gödöllő, Nagykovácsi and Valkó), host plants (Lathyrus tuberosus, Medicago sativa, Taraxacum officinale, Trifolium pratense), sexes, and larvae in case of Aeolothrips intermedius. On the basis of the total of 103 fragments generated by 15 RAPD primers the genetic distances were calculated by cluster analysis using simple matching method. The dendrogram resulted in two main groups: Aeolothripidae (Aeolothrips intermedius) and Thripidae (Frankliniella intonsa, Kakothrips robustus, Odontothrips confusus, Thrips dilatatus and T. tabaci). Within the family Thripidae two subgroups were observed including (i) F. intonsa, T. dilatatus and T. tabaci, and (ii) K. robustus and O. confusus. Two population-specific and one sex-linked fragments were identified by the RAPD primers, OPQ14, NO11 and OPA08, respectively.

Keywords

Molecular polymorphism RAPD Aeolothripidae Thripidae Thysanoptera 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cenis, J. L., Beitia, F. (1994) Aplicacion de la tecnica RAPD-PCR (adn polimorfico amplificado al azar) a la identificacion de insectos. Investigación Agraria: Producción y protección vegetales 9, 289–297.Google Scholar
  2. 2.
    Doyle, J. J., Doyle, J. L. (1990) Isolation of plant DNA from fresh tissue. Focus 12, 13–15.Google Scholar
  3. 3.
    Hartl, D. L., Brown, S. W. (1970) The origin of male haploid genetic systems and their expected sex ratio. Theoretical Population Biology 1, 165–190.CrossRefGoogle Scholar
  4. 4.
    Jenser, G. (1982) Fauna Hungariae: Tripszek-Thysanoptera. Volume 13. Akadémiai Kiadó, Budapest.Google Scholar
  5. 5.
    Klein, M., Gafni, R. (1996) Morphological and molecular variations in thrips populations collected on onion plants in Israel. Folia Entomologica Hungarica 57, 57–59.Google Scholar
  6. 6.
    Kraus, M., Schreiter, G., Moritz, G. (1999) Moleculargenetic studies of thrips species. In: Vierbergen, G., Tunç, I. (eds), Proceedings of the sixth International Symposium on Thysanoptera. Antalya, pp. 77–80.Google Scholar
  7. 7.
    Mendel, Z., Nestel, D., Gafni, R. (1994) Examination of the origin of the Israeli population of the Matsucoccus josephi (Homoptera: Matsucoccidae) using random amplified polymorphic DNA-poly-merase chain reaction. Annals of the Entomological Society of America 87, 165–169.CrossRefGoogle Scholar
  8. 8.
    Mound, L. A., Kibby, G. (1998) Thysanoptera: An Identification Guide. CAB International, Wallingford.Google Scholar
  9. 9.
    Perring, T. M., Cooper, A. D., Rodriguez, R. J., Farrar, A. A., Bellows, Jr. T. S. (1993) Identification of whitefly species by genomic and behavioural studies. Science 259, 74–77.CrossRefGoogle Scholar
  10. 10.
    Podani, J. (1993) Syn-Tax-pc Version 5.0: Computer programs for multivariate data. Scientia Publishing, BudapestGoogle Scholar
  11. 11.
    Riesler, H., Kempter, E. (1961) Die Haploidie der Männchen und die Endopolyploidie in einigen Geweben von Haplothrips (Thysanoptera). Chromosoma 12, 351–361.CrossRefGoogle Scholar
  12. 12.
    White, M. J. D. (1973) Animal Cytology and Evolution. 3rd Edition. Cambridge University Press, Cambridge.Google Scholar
  13. 13.
    Whiting, P. W. (1945) The evolution of male haploidy. Queensland Review of Biology 20, 427–500.Google Scholar
  14. 14.
    Wijkamp, I., Almarza, N., Goldbach, R., Peters, D. (1995) Distinct level of specificity in thrips transmission of tospoviruses. Phytopathology 85, 1069–1074.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2002

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • K. Bayar
    • 1
    Email author
  • O. Törjék
    • 2
  • Erzsébet Kiss
    • 2
  • G. Gyulai
    • 2
  • L. Heszky
    • 2
  1. 1.Department of Plant ProtectionSzent István UniversityGödöllőHungary
  2. 2.Department of Genetics and Plant BreedingSzent István UniversityGödöllőHungary

Personalised recommendations