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Acta Biologica Hungarica

, Volume 52, Issue 2–3, pp 345–354 | Cite as

Evaluation of Genetic Diversity of Poplar Genotypes by RAPD and AP-PCR Analysis

  • O. Törjék
  • Erzsébet Kiss
  • J. Kiss
  • M. Kondrák
  • G. Gyulai
  • J. Gergácz
  • L. HeszkyEmail author
Article

Abstract

RAPD (randomly amplified polymorphic DNA) and AP-PCR (arbitrarily primed PCR) were utilized to establish the genetic diversity of 19 Populus genotypes. A set of 40 primers of random sequence was tested, of which 35 exhibited polymorphism. Eighteen primers generated 162 easily detectable bands between 250 and 2500 base pairs in size, sufficient to distinguish between the genotypes. Similarity measures, cluster and multidimensional scaling analysis were performed to evaluate the RAPD and AP-PCR data. Our study demonstrated that in most instances similarity in the RAPD and AP-PCR banding patterns reflected the relationship due to origin. Nineteen primers gave a species or hybrid-specific pattern. One primer generated a specific pattern in P. euramericana. Ten primers produced specific fragments in VIF (P. alba), 4 primers in KOR (P. pyramidalis × P. berolinensis) and 4 primers in UNA and RAS (P. trichocarpa × P. deltoides). The results of this study demonstrated that RAPD or AP-PCR can be used to distinguish between poplar genotypes.

Keywords

Poplar RAPD AP-PCR polymorphism cluster analysis 

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Copyright information

© Akadémiai Kiadó, Budapest 2001

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • O. Törjék
    • 1
  • Erzsébet Kiss
    • 1
  • J. Kiss
    • 1
  • M. Kondrák
    • 1
  • G. Gyulai
    • 1
  • J. Gergácz
    • 2
  • L. Heszky
    • 1
    Email author
  1. 1.Department of Genetics and Plant BreedingSzent István UniversityGödöllőHungary
  2. 2.Sárvár Research StationHungarian Forest Research InstituteSárvárHungary

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